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P. S. Chen

Bio: P. S. Chen is an academic researcher. The author has contributed to research in topics: Ascorbic acid & Phosphorus. The author has an hindex of 1, co-authored 1 publications receiving 7063 citations.

Papers
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Journal ArticleDOI
25 Dec 2022
TL;DR: For example, this article showed that 80% of the users were satisfied with the performance of the proposed scheme, while only 5% were dissatisfied with the quality of the resulting results.
Abstract: 目的: 探讨自制平头注射针在肠憩室粪石嵌顿松解中的应用价值。 方法: 本研究为回顾性队列研究。回顾性收集汕头市中心医院内镜中心,2018年1月至2022年5月行结直肠憩室粪石嵌顿内镜下粪石松解术患者的资料。纳入门诊肠镜检查发现结直肠憩室并合并粪石嵌顿者,排除合并基础疾病无法耐受较长时间的内镜操作者。由于松解方法的改进,肠憩室粪石嵌顿发病率较低的原因,本研究根据自制平头注射针使用前后(2020年10月)的时间点作为分组条件,分为活检钳松解组(126例,2018年1月至2020年9月)和平头注射针水压松解组(91例,2020年10月至2022年5月),两组年龄、性别、憩室粪石嵌顿部位和情况以及症状构成方面差异无统计学意义(P>0.05)。本研究的观察指标为两组患者的松解情况(包括松解时间和松解成功率)和并发症发生情况。 结果: 平头注射针水压松解组松解时间短于活检钳松解组[10(10,15)s比100(80,140)s,Z=-11.326,P<0.001]。活检钳松解组肠憩室粪石嵌顿松解失败43例(34.1%),部分松解(粪石部分取出但无法完全取出)34例(27.0%),松解成功49例(38.9%);平头注射针水压松解组91例(100%)全部松解成功,无松解失败或部分松解病例;平头注射针水压松解组松解成功率较活检钳松解组更高,差异有统计学意义(Z=9.048,P<0.001)。两组患者在松解过程中,均未出现术中及术后的出血和穿孔。活检钳松解组有7例因开口较小且粪石体积较大,而行反复操作,导致憩室周围渗血、水肿、血肿,无需内镜下干预。平头注射针水压松解组发生3例黏膜下注射失误,但最终成功松解粪石,未造成术中术后出血穿孔等并发症。 结论: 平头注射针较活检钳对肠憩室粪石嵌顿松解的成功率更高,操作时间更短,有较大的实际意义和推广价值。.

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Journal ArticleDOI
TL;DR: The phosphomolybdenum method is routinely applied in the laboratory to evaluate the total antioxidant capacity of plant extracts and to determine vitamin E in a variety of grains and seeds, including corn and soybean.

4,644 citations

Book ChapterDOI
TL;DR: The method is about seven times as sensitive as the Fiske–SubbaRow procedure and involves less pipetting, but it is not very satisfactory for determining inorganic phosphate if labile phosphate esters are present in large excess.
Abstract: Publisher Summary This chapter discusses the assay of inorganic phosphate, total phosphate, and phosphatases. The phosphomolybdate complex is reduced by ascorbic acid. The method is about seven times as sensitive as the Fiske–SubbaRow procedure and involves less pipetting. One can easily determine 0.01 micromole of phosphate. Pyrophosphate breaks down about 5% in the method and compounds such as glucose 1-phosphate also break down somewhat, so that the method is not very satisfactory for determining inorganic phosphate if labile phosphate esters are present in large excess. The sample of organic phosphate and a drop of magnesium nitrate solution in a small test tube are taken to dryness by shaking the tube in flame. The ashing procedure is rapid and is good for various biological materials and phosphate esters such as nucleic acid, carbohydrate phosphate esters, viruses, and phospholipids. The assay method of phosphatases for inorganic phosphate can be used as an assay for phosphatases hydrolyzing stable phosphate esters such as glucose-6-phosphate, ribose-5-phosphate, and histidinol phosphate. The enzyme incubation can be stopped with the one ascorbic-molybdate solution thus avoiding an extra pipetting.

3,256 citations

Journal ArticleDOI
TL;DR: The cations of T4 phage have been examined and a balance has been obtained between total cations and total DNA anions and the replacement of the normal polyamines suggested that the polyamines may be acting as nonspecific cations.

2,468 citations

Journal ArticleDOI
TL;DR: It is shown that minimal levels of serum cholesterol were achieved with oils with an iodine number of about 100, and that more highly unsaturated oils were not more effective, which implies that monounsaturated acids are half as effective as the diene, linoleic acid, the primary polyunsaturated acid in vegetable oils.

1,480 citations

Journal ArticleDOI
TL;DR: The kinetics and tissue distribution of the microsomal heme oxygenase suggest that it is of major importance in the physiological degradation of hemoglobin and other hemoproteins to bile pigment.

1,315 citations