Asymmetric fluorination, trifluoromethylation, and perfluoroalkylation reactions.

Update 1 of: Asymmetric Fluorination, Trifluoromethylation, and Perfluoroalkylation Reactions

The coordination chemistry of the amidine ligand

Structure, Reactivity, and Chemistry of Fluoroalkyl Radicals.

Sirodesmin PL is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). This phytotoxin belongs to the epipolythiodioxopiperazine (ETP) class of toxins produced by fungi including mammalian and plant pathogens. We report the cloning of a cluster of genes with predicted roles in the biosynthesis of sirodesmin PL and show via gene disruption that one of these genes (encoding a two-module non-ribosomal peptide synthetase) is essential for sirodesmin PL biosynthesis. Of the nine genes in the cluster tested, all are co-regulated with the production of sirodesmin PL in culture. A similar cluster is present in the genome of the opportunistic human pathogen Aspergillus fumigatus and is most likely responsible for the production of gliotoxin, which is also an ETP. Homologues of the genes in the cluster were also identified in expressed sequence tags of the ETP producing fungus Chaetomium globosum. Two other fungi with publicly available genome sequences, Magnaporthe grisea and Fusarium graminearum, had similar gene clusters. A comparative analysis of all four clusters is presented. This is the first report of the genes responsible for the biosynthesis of an ETP.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2004.04215.x

The sirodesmin biosynthetic gene cluster of the plant pathogenic fungus Leptosphaeria maculans.

Synthesis of 6-methylthiopenicillins and 7-heteroatom-substituted cephalosporins.

Biosynthetic studies on gliotoxin using stable isotopes and mass spectral methods.

Mass Spectral Studies. III. Fragmentation of Aromatic Amides

Synthesis of 2-, 4-, and 7-methylthio-substituted cephalosporins

The structure of lochnericine and lochnerinine

P. T. Funke

Papers

Synthesis of 6-methylthiopenicillins and 7-heteroatom-substituted cephalosporins.

Biosynthetic studies on gliotoxin using stable isotopes and mass spectral methods.

Mass Spectral Studies. III. Fragmentation of Aromatic Amides

Synthesis of 2-, 4-, and 7-methylthio-substituted cephalosporins

The structure of lochnericine and lochnerinine