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Pamela C. Ronald

Bio: Pamela C. Ronald is an academic researcher from University of California, Davis. The author has contributed to research in topics: Xanthomonas oryzae & Xanthomonas oryzae pv. oryzae. The author has an hindex of 83, co-authored 315 publications receiving 27600 citations. Previous affiliations of Pamela C. Ronald include Energy Institute & International Rice Research Institute.


Papers
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Journal ArticleDOI
15 Dec 1995-Science
TL;DR: The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. race 6, was isolated by positional cloning and the sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response.
Abstract: The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. oryzae race 6, was isolated by positional cloning. Fifty transgenic rice plants carrying the cloned Xa21 gene display high levels of resistance to the pathogen. The sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response. Characterization of Xa21 should facilitate understanding of plant disease resistance and lead to engineered resistance in rice.

2,023 citations

Journal ArticleDOI
John P. Vogel1, David F. Garvin2, Todd C. Mockler2, Jeremy Schmutz, Daniel S. Rokhsar3, Michael W. Bevan4, Kerrie Barry5, Susan Lucas5, Miranda Harmon-Smith5, Kathleen Lail5, Hope Tice5, Jane Grimwood, Neil McKenzie4, Naxin Huo6, Yong Q. Gu6, Gerard R. Lazo6, Olin D. Anderson6, Frank M. You7, Ming-Cheng Luo7, Jan Dvorak7, Jonathan M. Wright4, Melanie Febrer4, Dominika Idziak8, Robert Hasterok8, Erika Lindquist5, Mei Wang5, Samuel E. Fox2, Henry D. Priest2, Sergei A. Filichkin2, Scott A. Givan2, Douglas W. Bryant2, Jeff H. Chang2, Haiyan Wu9, Wei Wu10, An-Ping Hsia10, Patrick S. Schnable9, Anantharaman Kalyanaraman11, Brad Barbazuk12, Todd P. Michael, Samuel P. Hazen13, Jennifer N. Bragg6, Debbie Laudencia-Chingcuanco6, Yiqun Weng14, Georg Haberer, Manuel Spannagl, Klaus F. X. Mayer, Thomas Rattei15, Therese Mitros3, Sang-Jik Lee16, Jocelyn K. C. Rose16, Lukas A. Mueller16, Thomas L. York16, Thomas Wicker17, Jan P. Buchmann17, Jaakko Tanskanen18, Alan H. Schulman18, Heidrun Gundlach, Michael W. Bevan4, Antonio Costa de Oliveira19, Luciano da C. Maia19, William R. Belknap6, Ning Jiang, Jinsheng Lai9, Liucun Zhu20, Jianxin Ma20, Cheng Sun21, Ellen J. Pritham21, Jérôme Salse, Florent Murat, Michael Abrouk, Rémy Bruggmann, Joachim Messing, Noah Fahlgren2, Christopher M. Sullivan2, James C. Carrington2, Elisabeth J. Chapman, Greg D. May22, Jixian Zhai23, Matthias Ganssmann23, Sai Guna Ranjan Gurazada23, Marcelo A German23, Blake C. Meyers23, Pamela J. Green23, Ludmila Tyler3, Jiajie Wu7, James A. Thomson6, Shan Chen13, Henrik Vibe Scheller24, Jesper Harholt25, Peter Ulvskov25, Jeffrey A. Kimbrel2, Laura E. Bartley24, Peijian Cao24, Ki-Hong Jung26, Manoj Sharma24, Miguel E. Vega-Sánchez24, Pamela C. Ronald24, Chris Dardick6, Stefanie De Bodt27, Wim Verelst27, Dirk Inzé27, Maren Heese28, Arp Schnittger28, Xiaohan Yang29, Udaya C. Kalluri29, Gerald A. Tuskan29, Zhihua Hua14, Richard D. Vierstra14, Yu Cui9, Shuhong Ouyang9, Qixin Sun9, Zhiyong Liu9, Alper Yilmaz30, Erich Grotewold30, Richard Sibout31, Kian Hématy31, Grégory Mouille31, Herman Höfte31, Todd P. Michael, Jérôme Pelloux32, Devin O'Connor3, James C. Schnable3, Scott C. Rowe3, Frank G. Harmon3, Cynthia L. Cass33, John C. Sedbrook33, Mary E. Byrne4, Sean Walsh4, Janet Higgins4, Pinghua Li16, Thomas P. Brutnell16, Turgay Unver34, Hikmet Budak34, Harry Belcram, Mathieu Charles, Boulos Chalhoub, Ivan Baxter35 
11 Feb 2010-Nature
TL;DR: The high-quality genome sequence will help Brachypodium reach its potential as an important model system for developing new energy and food crops and establishes a template for analysis of the large genomes of economically important pooid grasses such as wheat.
Abstract: Three subfamilies of grasses, the Ehrhartoideae, Panicoideae and Pooideae, provide the bulk of human nutrition and are poised to become major sources of renewable energy. Here we describe the genome sequence of the wild grass Brachypodium distachyon (Brachypodium), which is, to our knowledge, the first member of the Pooideae subfamily to be sequenced. Comparison of the Brachypodium, rice and sorghum genomes shows a precise history of genome evolution across a broad diversity of the grasses, and establishes a template for analysis of the large genomes of economically important pooid grasses such as wheat. The high-quality genome sequence, coupled with ease of cultivation and transformation, small size and rapid life cycle, will help Brachypodium reach its potential as an important model system for developing new energy and food crops.

1,603 citations

Journal ArticleDOI
TL;DR: A survey of bacterial pathologists asked them to nominate the bacterial pathogens they would place in a 'Top 10' based on scientific/economic importance, and a short section is presented on each bacterium in the Top 10 list and its importance, with the intention of initiating discussion and debate amongst the plant bacteriology community.
Abstract: Many plant bacteriologists, if not all, feel that their particular microbe should appear in any list of the most important bacterial plant pathogens. However, to our knowledge, no such list exists. The aim of this review was to survey all bacterial pathologists with an association with the journal Molecular Plant Pathology and ask them to nominate the bacterial pathogens they would place in a 'Top 10' based on scientific/economic importance. The survey generated 458 votes from the international community, and allowed the construction of a Top 10 bacterial plant pathogen list. The list includes, in rank order: (1) Pseudomonas syringae pathovars; (2) Ralstonia solanacearum; (3) Agrobacterium tumefaciens; (4) Xanthomonas oryzae pv. oryzae; (5) Xanthomonas campestris pathovars; (6) Xanthomonas axonopodis pathovars; (7) Erwinia amylovora; (8) Xylella fastidiosa; (9) Dickeya (dadantii and solani); (10) Pectobacterium carotovorum (and Pectobacterium atrosepticum). Bacteria garnering honourable mentions for just missing out on the Top 10 include Clavibacter michiganensis (michiganensis and sepedonicus), Pseudomonas savastanoi and Candidatus Liberibacter asiaticus. This review article presents a short section on each bacterium in the Top 10 list and its importance, with the intention of initiating discussion and debate amongst the plant bacteriology community, as well as laying down a benchmark. It will be interesting to see, in future years, how perceptions change and which bacterial pathogens enter and leave the Top 10.

1,479 citations

Journal ArticleDOI
10 Aug 2006-Nature
TL;DR: The identification of a cluster of three genes at the Sub1 locus, encoding putative ethylene response factors, indicates that Sub1A-1 is a primary determinant of submergence tolerance in O. sativa ssp.
Abstract: Most Oryza sativa cultivars die within a week of complete submergence--a major constraint to rice production in south and southeast Asia that causes annual losses of over US 1 billion dollars and affects disproportionately the poorest farmers in the world. A few cultivars, such as the O. sativa ssp. indica cultivar FR13A, are highly tolerant and survive up to two weeks of complete submergence owing to a major quantitative trait locus designated Submergence 1 (Sub1) near the centromere of chromosome 9 (refs 3, 4, 5-6). Here we describe the identification of a cluster of three genes at the Sub1 locus, encoding putative ethylene response factors. Two of these genes, Sub1B and Sub1C, are invariably present in the Sub1 region of all rice accessions analysed. In contrast, the presence of Sub1A is variable. A survey identified two alleles within those indica varieties that possess this gene: a tolerance-specific allele named Sub1A-1 and an intolerance-specific allele named Sub1A-2. Overexpression of Sub1A-1 in a submergence-intolerant O. sativa ssp. japonica conferred enhanced tolerance to the plants, downregulation of Sub1C and upregulation of Alcohol dehydrogenase 1 (Adh1), indicating that Sub1A-1 is a primary determinant of submergence tolerance. The FR13A Sub1 locus was introgressed into a widely grown Asian rice cultivar using marker-assisted selection. The new variety maintains the high yield and other agronomic properties of the recurrent parent and is tolerant to submergence. Cultivation of this variety is expected to provide protection against damaging floods and increase crop security for farmers.

1,371 citations

Journal ArticleDOI
01 Dec 1994-Genetics
TL;DR: A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs) derived from a backcross between cultivated rice and its wild African relative, Oryza longistaminata.
Abstract: A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F2 population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed.

874 citations


Cited by
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28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

Journal ArticleDOI
16 Nov 2006-Nature
TL;DR: A detailed understanding of plant immune function will underpin crop improvement for food, fibre and biofuels production and provide extraordinary insights into molecular recognition, cell biology and evolution across biological kingdoms.
Abstract: Many plant-associated microbes are pathogens that impair plant growth and reproduction. Plants respond to infection using a two-branched innate immune system. The first branch recognizes and responds to molecules common to many classes of microbes, including non-pathogens. The second responds to pathogen virulence factors, either directly or through their effects on host targets. These plant immune systems, and the pathogen molecules to which they respond, provide extraordinary insights into molecular recognition, cell biology and evolution across biological kingdoms. A detailed understanding of plant immune function will underpin crop improvement for food, fibre and biofuels production.

10,539 citations

Journal ArticleDOI
10 Mar 1970

8,159 citations

01 Aug 2000
TL;DR: Assessment of medical technology in the context of commercialization with Bioentrepreneur course, which addresses many issues unique to biomedical products.
Abstract: BIOE 402. Medical Technology Assessment. 2 or 3 hours. Bioentrepreneur course. Assessment of medical technology in the context of commercialization. Objectives, competition, market share, funding, pricing, manufacturing, growth, and intellectual property; many issues unique to biomedical products. Course Information: 2 undergraduate hours. 3 graduate hours. Prerequisite(s): Junior standing or above and consent of the instructor.

4,833 citations

Journal ArticleDOI
05 Apr 2002-Science
TL;DR: A draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp.indica, by whole-genome shotgun sequencing is produced, with a large proportion of rice genes with no recognizable homologs due to a gradient in the GC content of rice coding sequences.
Abstract: We have produced a draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp. indica, by whole-genome shotgun sequencing. The genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. Functional coverage in the assembled sequences was 92.0%. About 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. Although 80.6% of predicted Arabidopsis thaliana genes had a homolog in rice, only 49.4% of predicted rice genes had a homolog in A. thaliana. The large proportion of rice genes with no recognizable homologs is due to a gradient in the GC-content of rice coding sequences.

4,064 citations