Pankaj B Nariya

Bio: Pankaj B Nariya is an academic researcher from Saurashtra University. The author has contributed to research in topics: DPPH & Gallic acid. The author has an hindex of 6, co-authored 19 publications receiving 167 citations. Previous affiliations of Pankaj B Nariya include Gujarat Ayurved University & RK University.

In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn.

Abstract: The present study is aimed to investigate antioxidant activity of the extracts of Cassia fistula Linn. (Leguminosae) fruit pulp. Cassia fistula Linn., a Indian Laburnum, is widely cultivated in various countries and different continents including Asia, Mauritius, South Africa, Mexico, China, West Indies, East Africa and Brazil as an ornamental tree for its beautiful bunches of yellow flowers and also used in traditional medicine for several indications. The primary phytochemical study and in vitro antioxidant study was performed on hydro alcoholic extract of fruit pulp. Phytochemical screening of the plant has shown the presence of phenolic compounds, fatty acids, flavonoids, tannins and glycosides. Phenolic content was measured using Folin-Ciocalteu reagent and was calculated as gallic acid equivalents. Antiradical activity of hydro alcoholic extract was measured by DPPH (2,2-diphenyl-1- picrylhydrazyl) assay and was compared to ascorbic acid. Ferric reducing power of the extract was also evaluated by Oyaizu method. In the present study, three methods were used for evaluation of antioxidant activity. First two methods were for direct measurement of radical scavenging activity and third method to evaluate the reducing power. Results indicate that hydro alcoholic fruit pulp extracts have marked amount of total phenols which could be responsible for the antioxidant activity. These in vitro assays indicate that this plant extract is a significant source of natural antioxidant, Cassia fistula fruit pulp extract shows lower activity in DPPH and total phenol content as compared with standard which might be helpful in preventing the progress of various oxidative stresses.

In vitro evaluation of antioxidant activity of Cordia dichotoma (Forst f.) bark

Abstract: Cordia dichotoma Forst. f. bark, identified as botanical source of Shleshmataka in Ayurvedic pharmacopoeia. Present investigation was undertaken to evaluate possible antioxidant potential of methanolic and butanol extract of C. dichotoma bark. In vitro antioxidant activity of methanolic and butanol extract was determined by 1,1, diphenyl-2, picrylhydrazyl (DPPH) free radical scavenging assay. The extracts were also evaluated for their phenolic contents and antioxidant activity. Phenolic content was measured using Folin-Ciocalteu reagent and was calculated as Gallic acid equivalents. Antiradical activity of methanolic extract was measured by DPPH assay and was compared to ascorbic acid and ferric reducing power of the extract was evaluated by Oyaizu method. In the present study three in vitro models were used to evaluate antioxidant activity. The first two methods were for direct measurement of radical scavenging activity and remaining one method evaluated the reducing power. The present study revealed that the C. dichotoma bark has significant radical scavenging activity.

In vitro antibacterial and antifungal activities of Cassia fistula Linn. fruit pulp extracts

Abstract: Aim of the study is to assess the antimicrobial activity Cassia fistula fruit pulp extracts on some bacterial and fungal strains. Hydro alcohol and chloroform extracts of Cassia fistula fruit pulp were evaluated for the potential antimicrobial activity. The antimicrobial activity was determined in both the extracts using the agar disc diffusion method. Extracts were effective on tested microorganisms. The antibacterial and antifungal activities of solvent extracts (5, 25, 50, 100, 250 μg/mL) of C. fistula were tested against two gram positive, two gram negative human pathogenic bacteria and three fungi, respectively. Crude extracts of C. fistula exhibited moderate to strong activity against most of the bacteria tested. The tested bacterial strains were Staphylococcus aureus, Streptococcus pyogenes, Escherichia coil, Pseudomonas aeruginosa, and fungal strains were Aspergillus. niger, Aspergillus. clavatus, Candida albicans. The antibacterial potential of the extracts were found to be dose dependent. The antibacterial activities of the C. fistula were due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

Antimicrobial and antifungal activities of Cordia dichotoma (Forster F.) bark extracts

Abstract: Cordia dichotoma Forst.f. bark, identified as botanical source of Shlesmataka in Ayurvedic pharmacopoeias. Present study was carried out with an objective to investigate the antibacterial and antifungal potentials of Cordia dichotoma bark. Antibacterial activity of methanol and butanol extracts of the bark was carried out against two gram negative bacteria (Escherichia coli, and Pseudomonas aeruginosa) and two Gram positive bacteria (St. pyogenes and Staphylococcus aureus). The antifungal activity of the extracts was carried out against three common pathogenic fungi (Aspergillus niger, A.clavatus, and Candida albicans). Zone of inhibition of extracts was compared with that of different standards like Amplicilline, Ciprofloxacin, Norfloxacin and Chloramphenicol for antibacterial activity and Nystain and Greseofulvin for antifungal activity. The extracts showed remarkable inhibition of zone of bacterial growth and fungal growth and the results obtained were comparable with that of standards drugs against the organisms tested. The activity of extracts increased linearly with increase in concentration of extract (mg/ml). The results showed the antibacterial and antifungal activity against the organisms tested.

Evaluation of in-vitro antioxidant activity of panchagavya: a traditional ayurvedic preparation

Abstract: Panchagavya, a classical Ayurvedic preparation, was evaluated for its antioxidant potential by HPTLC-DPPH bioautography method as well as assays for Ferric reducing antioxidant power (FRAP), DPPH – free radical scavenging activity (DPPH) and Superoxide radical scavenging activity. In addition total phenolic content was also estimated which was in fairly good amount. HPTLC-DPPH bioautography study revealed the presence of several antioxidant compounds in Panchagavya. In all the assays performed, it showed considerable antioxidant activity. On comparison of the data of three different batches of the samples studied, it showed 98.3 - 99.8% correlation between total phenolic content, FRAP and DPPH assays.

Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae).

Abstract: Objectives Cordia (family Boraginaceae) is a genus of deciduous flowering trees or shrubs comprising more than 300 species distributed widely in the tropical regions. The aim of this review was to provide exhaustive scientific information on traditional uses, phytochemistry and pharmacological activities of the 36 important species with medicinal value from the genus Cordia, to divulge prospects for further research on its therapeutic potential. Key findings Leaves, fruit, bark and seed of a majority of the species were found to possess abundant ethnomedicinal value, but leaves were found to be used most frequently to treat many ailments such as respiratory disorders, stomach pain, wound, inflammation, myalgia, cough, dysentery and diarrhoea. The phytochemical investigation of 36 species resulted in isolation of 293 chemical constituents from various chemical classes. The crude extracts, fractions, essential oils and pure compounds isolated from various Cordia species were reported to have a varied range of pharmacological activities. Summary Many of the traditional uses of the genus Cordia were supported by the results obtained from pharmacological studies performed using various extracts or pure compounds. More attention should be given to the biological evaluation using pure phytochemicals and to identify the mechanism of actions and exploring this genus for new drug discovery.

Plant profile, phytochemistry and pharmacology of Cordia dichotoma (Indian cherry): a review.

Abstract: More than half of the world's population relies on the traditional medicine and major role of the traditional medicine including the use of plant extract and their active constituents. Among them, Cordia dichotoma Forst., a small to moderate size plant of family Boragenaceae, commonly called bhokar, lasura, gonda, Indian cherry and shlesmataka. Plant parts such as leaves, fruit, bark and seed have been reported for possessing antidiabetic, antiulcer, anti-inflammatory, immune-modulator and analgesic activity. Screening of fruit, leaves and seed shows the presence of pyrrolizidine alkaloids, coumarins, flavonoids, saponins, terpenes and sterols. Present review focuses on details of geographical distribution, physicochemical parameters, phytoconstituents and pharmacological properties of Cordia dichotoma reported so far.

agros dictionary of medicinal plants

Abstract: agros dictionary of medicinal plants , agros dictionary of medicinal plants , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

Mechanisms of halotolerant plant growth promoting Alcaligenes sp. involved in salt tolerance and enhancement of the growth of rice under salinity stress.

Abstract: In the present study halotolerant bacteria were isolated from saline soil (EC ~ 11.9). Based on salt tolerance and plant growth promoting characteristics isolate AF7 was selected for further study. It was identified as Alcaligenes sp. on the basis of protein profiling and 16S rRNA sequence homology. Interestingly, AF7 showed diverse PGP characters at different salinity levels. While phosphate solubilization activity was expressed up to 300 mM NaCl, siderophore production was shown up to 700 mM, zinc solubilization up to 1000 mM and indole acetic acid (IAA), gibberellic acid (GA) and exopolysaccharides (EPS) production were depicted till 1400 mM. Correlative and regression analysis suggested positive relation between IAA, GA, EPS, siderophore production and zinc solubilization capability of AF7 and salinity up to 300 mM NaCl. EPS was found to be the most significant response and there was 263% increment in presence of 300 mM NaCl when compared to non-saline control. Analysis also showed that while growth promoting attributes were significant up to a threshold salinity level, further increasing the stress deviates the mechanism towards survival involving proline, antioxidant and hydroxyl scavenging activities. Combination of halotolerant AF7 and EPS showed more than twofold increase in the vegetative growth parameters of rice at ~ 170 mM NaCl (EC 9 dS/m). The study shows the mechanisms/metabolites of the plant growth promoting bacterium (PGPB) AF7 prominently involved during the salinity stress. Study also proves that novel bioformulations can be developed by integrative use of EPS and salt tolerant-PGPB which can be effective for saline soils.