Author
Patricia V.P. Sales
Bio: Patricia V.P. Sales is an academic researcher from Federal University of Ceará. The author has contributed to research in topics: Affinity chromatography & Lectin. The author has an hindex of 1, co-authored 1 publications receiving 64 citations.
Topics: Affinity chromatography, Lectin
Papers
More filters
TL;DR: A lectin from Vatairea macrocarpa Duke seeds (VML) was isolated using affinity chromatography on a guar gum column and size-exclusion chromatography indicated that VML is predominantly a dimeric protein, although tetramers and larger aggregates were also present.
65 citations
Cited by
More filters
TL;DR: The results suggest a binding model in which lectin molecules “bind and jump” from α-GalNAc residue to α- GalNAc residues along the polypeptide chain of Tn-PSM before dissociating, which has important implications for the biological activities of mucins including those expressing the Tn cancer antigen.
91 citations
TL;DR: A novel plant lectin has been isolated from the seeds of Talisia esculenta and partially characterized and a search of sequence databases showed that the N-terminal sequence had no homology to any known protein.
77 citations
TL;DR: The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops.
Abstract: This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD50 and ED50 value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD50, whereas 0.2% KpLec produced an ED50. The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops. Keywords: Koelreuteria paniculata; N-acetylglucosamine binding; lecti...
66 citations
TL;DR: The results indicate that ConA and ConBr inhibited cell proliferation selectively in tumor cells and that apoptosis was the main death mechanism, and lectins can be considered a class of molecules with a high antitumor activity potential.
62 citations
TL;DR: The Origins and applications of lectins are described, highlighting the special features of this class of proteins, such as the carbohydrated‐binding domains and their importance in the development of affinity methodologies to increase and facilitate lectins purification.
Abstract: Lectins hold great promise not only as reagents for diagnostics and drug discovery but also as a novel class of biopharmaceutical products. In fact, new research directions in the last years have led to major developments in the uses of plant lectins as therapeutic agents against numerous diseases in an ageing society. It is even expected that lectins may occupy an important place in the biopharmaceutical industry next to monoclonal antibodies. All these new trends are placing a tremendous emphasis on the development of new approaches for faster lectins development, selection, and optimization, including alternatives methods of purification. This article reviews the isolation and purification methods used for lectins purification. Origins and applications of lectins are described, highlighting the special features of this class of proteins, such as the carbohydrated-binding domains and their importance in the development of affinity methodologies to increase and facilitate lectins purification. Published strategies for the purification of lectins from different sources are analyzed in relation to the purification methods used, their sequence, and the number of times they are used in a purification procedure. The purity of lectins is analyzed in relation to the average overall yield and purification factors obtained for each purification scheme for these proteins and the purification steps necessary. New directions are described for improving lectins separation and purification.
61 citations