Showing papers by "Paul G. Richardson published in 1984"

T‐Cell Proliferation and Expression of MHC Class II Antigens

Abstract: Two monoclonal antibodies to major histocompatibility complex (MHC) class II antigens, which in combination identify beta chains encoded by the SB and DR loci, were used to investigate which of these gene products were expressed at the cell surface of unstimulated T cells and at various stages of mitogen-induced T-cell maturation. In tests on blood lymphocytes from healthy donors 12% of T cells expressed class II antigens, but only SB antigens were expressed. During activation of T lymphocytes, SB-coded antigens were expressed before DR antigens, and the kinetics of SB expression correlated with the proliferative response of T cells. These results and consideration of recent reports from other laboratories lead us to suggest that SB-coded class II antigens play a role in T-cell proliferation.

Subclasses of IgG on the surface of human lymphocytes: a study with monoclonal antibodies.

Abstract: Cells from sIgG+ B lymphoblastoid cell lines, sIgG+ B cell neoplasms and from tonsils, adult and cord blood and fetal spleen were tested for IgG subclass expression using a panel of monoclonal antibodies. IgG1 was in all cases the commonest subclass except on blood lymphocytes, when approximately equal numbers of cells expressing IgG1 and IgG2 were found. No evidence for multiple subclass isotype expression was found.