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Showing papers by "Paul G. Richardson published in 1990"


Journal ArticleDOI
TL;DR: The results show that the oncogenicity of Rel proteins requires activation region I and suggest that the biological function of rel and dorsal proteins depends on transcription activation by this region.
Abstract: The mechanism by which the products of the v-rel oncogene, the corresponding c-rel proto-oncogene, and the related dorsal gene of Drosophila melanogaster exert their effects is not clear. Here we show that the v-rel, chicken c-rel, and dorsal proteins activated gene expression when fused to LexA sequences and bound to DNA upstream of target genes in Saccharomyces cerevisiae. We have defined two distinct activation regions in the c-rel protein. Region I, located in the amino-terminal half of rel and dorsal proteins, contains no stretches of glutamines, prolines, or acidic amino acids and therefore may be a novel activation domain. Lesions in the v-rel protein that diminished or abolished oncogenic transformation of avian spleen cells correspondingly affected transcription activation by region I. Region II, located in the carboxy terminus of the c-rel protein, is highly acidic. Region II is not present in the v-rel protein or in a transforming mutant derivative of the c-rel protein. Our results show that the oncogenicity of Rel proteins requires activation region I and suggest that the biological function of rel and dorsal proteins depends on transcription activation by this region.

114 citations


01 Jan 1990
TL;DR: In this article, the authors defined two distinct activation regions of the c-rel protein, Region I, located intheamino-terminal half of relanddorsal proteins, contains no stretches of glutamines, prolines, or acidic aminoacids and therefore may be a novel activation domain.
Abstract: Themechanism bywhichtheproducts ofthev-rel oncogene,thecorresponding c-rel proto-oncogene, andthe related dorsal gene ofDrosophila melanogaster exerttheir effects isnotclear. Herewe showthatthev-rel, chicken c-rel, anddorsal proteins activated geneexpression whenfused toLexAsequencesandboundtoDNA upstream oftarget genesinSaccharomyces cerevisiae. We havedefined twodistinct activation regions inthe c-rel protein. Region I,located intheamino-terminal half ofrelanddorsal proteins, contains no stretches of glutamines, prolines, oracidic aminoacids andtherefore may beanovel activation domain. Lesions inthev-rel protein thatdiminished orabolished oncogenic transformation ofavian spleen cells correspondingly affected transcription activation byregion I.Region II,located inthecarboxy terminus ofthec-rel protein, ishighly acidic. Region IIisnotpresent inthev-rel protein orinatransforming mutantderivative ofthec-rel protein. Ourresults showthattheoncogenicity ofRelproteins requires activation region Iandsuggest thatthe biological function ofrelanddorsal proteins depends on transcription activation bythis region.

92 citations