Showing papers by "Pedro J. J. Alvarez published in 2019"

The Technology Horizon for Photocatalytic Water Treatment: Sunrise or Sunset?

Abstract: Advanced oxidation processes via semiconductor photocatalysis for water treatment have been the subject of extensive research over the past three decades, producing many scientific reports focused on elucidating mechanisms and enhancing kinetics for the treatment of contaminants in water. Many of these reports imply that the ultimate goal of the research is to apply photocatalysis in municipal water treatment operations. However, this ignores immense technology transfer problems, perpetuating a widening gap between academic advocation and industrial application. In this Feature, we undertake a critical examination of the trajectory of photocatalytic water treatment research, assessing the viability of proposed applications and identifying those with the most promising future. Several strategies are proposed for scientists and engineers who aim to support research efforts to bring industrially relevant photocatalytic water treatment processes to fruition. Although the reassessed potential may not live up to initial academic hype, an unfavorable assessment in some areas does not preclude the transfer of photocatalysis for water treatment to other niche applications as the technology retains substantive and unique benefits.

Global diversity and biogeography of bacterial communities in wastewater treatment plants

Abstract: Microorganisms in wastewater treatment plants (WWTPs) are essential for water purification to protect public and environmental health. However, the diversity of microorganisms and the factors that control it are poorly understood. Using a systematic global-sampling effort, we analysed the 16S ribosomal RNA gene sequences from ~1,200 activated sludge samples taken from 269 WWTPs in 23 countries on 6 continents. Our analyses revealed that the global activated sludge bacterial communities contain ~1 billion bacterial phylotypes with a Poisson lognormal diversity distribution. Despite this high diversity, activated sludge has a small, global core bacterial community (n = 28 operational taxonomic units) that is strongly linked to activated sludge performance. Meta-analyses with global datasets associate the activated sludge microbiomes most closely to freshwater populations. In contrast to macroorganism diversity, activated sludge bacterial communities show no latitudinal gradient. Furthermore, their spatial turnover is scale-dependent and appears to be largely driven by stochastic processes (dispersal and drift), although deterministic factors (temperature and organic input) are also important. Our findings enhance our mechanistic understanding of the global diversity and biogeography of activated sludge bacterial communities within a theoretical ecology framework and have important implications for microbial ecology and wastewater treatment processes.

Cooperative Pollutant Adsorption and Persulfate-Driven Oxidation on Hierarchically Ordered Porous Carbon.

Abstract: This study presents a 3D hierarchically ordered porous carbon material (HOPC) that simultaneously achieves efficient adsorption of a range of water pollutants as well as catalytic oxidation of adsorbed pollutants. High adsorption capacity and rapid adsorption kinetics are attributed to the hydrophobic nature of the carbon substrate, the large surface area due to high porosity, and the relatively uniform size of pores that comprise the structure. The oxidative degradation is achieved by efficient mediation of electron transfer from pollutants to persulfate through the sp2-hybridized carbon and nitrogen network. As the persulfate activation and pollutant oxidation do not involve reactive radicals, oxidative degradation of the adsorbent is prevented, which has been a primary concern when adsorption and oxidation are combined either to regenerate adsorbate or to enhance oxidation performance. Batch tests showed that near complete removal of various recalcitrant micropollutants can be achieved within a short time (less than 1 min) even when treating a complex water matrix, as pollutants are concentrated on the surface of HOPC, where their oxidation is catalyzed.

In situ remediation of subsurface contamination: opportunities and challenges for nanotechnology and advanced materials

Abstract: Complex subsurface contamination domains and limited efficacy of existing treatment approaches pose significant challenges to site remediation and underscore the need for technological innovation to develop cost-effective remedies. Here, we discuss opportunities for nanotechnology-enabled in situ remediation technologies to address soil and groundwater contamination. The discussion covers candidate nanomaterials, applications of nanomaterials to complement existing remediation approaches and address emerging contaminants, as well as the potential barriers for implementation and strategies and research needs to overcome these barriers. Promising nanomaterials in subsurface remediation include multi-functional nanocomposites for synergistic contaminant sequestration and degradation, selective adsorbents and catalysts, nano-tracers for subsurface contaminant delineation, and slow-release reagents enabled by stimuli-responsive nanomaterials. Limitations on mixing and transport of nanomaterials in the subsurface are severe constraints for in situ applications of these materials. Mixing enhancements are needed to overcome transport limitations in laminar flow environments. Reactive nanomaterials may be generated in situ to remediate contamination in low hydraulic conductivity zones. Overall, nano-enabled remediation technologies may improve remediation performance for a broad range of legacy and emerging contaminants. These technologies should continue to be developed and tested to discern theoretical hypotheses from feasible opportunities, and to establish realistic performance expectations for in situ remediation techniques using engineered nanomaterials alone or in combination with other technologies.

Going Viral: Emerging Opportunities for Phage-Based Bacterial Control in Water Treatment and Reuse.

Abstract: ConspectusWater security to protect human lives and support sustainable development is one of the greatest global challenges of this century. While a myriad of water pollutants can impact public health, the greatest threat arises from pathogenic bacteria that can be harbored in different components of water treatment, distribution, and reuse systems. Bacterial biofilms can also promote water infrastructure corrosion and biofouling, which substantially increase the cost and complexity of many critical operations.Conventional disinfection and microbial control approaches are often insufficient to keep up with the increasing complexity and renewed relevance of this pressing challenge. For example, common disinfectants cannot easily penetrate and eradicate biofilms, and are also relatively ineffective against resistant microorganisms. The use of chemical disinfectants is also curtailed by regulations aimed at minimizing the formation of harmful disinfection byproducts. Furthermore, disinfectants cannot be use...

Pilot-Scale Pyrolytic Remediation of Crude-Oil-Contaminated Soil in a Continuously-Fed Reactor: Treatment Intensity Trade-Offs.

Abstract: Pyrolytic treatment offers the potential for the rapid remediation of contaminated soils. However, soil fertility restoration can be highly variable, underscoring the need to understand how treatment conditions affect soil detoxification and the ability to support plant growth. We report here the first pilot-scale study of pyrolytic remediation of crude-oil-contaminated soil using a continuously fed rotary kiln reactor. Treatment at 420 °C with only 15 min of residence time resulted in high removal efficiencies for both total petroleum hydrocarbons (TPH) (99.9%) and polycyclic aromatic hydrocarbons (PAHs) (94.5%) and restored fertility to clean soil levels (i.e., Lactuca sativa biomass dry weight yield after 21 days increased from 3.0 ± 0.3 mg for contaminated soil to 8.8 ± 1.1 mg for treated soil, which is similar to 9.0 ± 0.7 mg for uncontaminated soil). Viability assays with a human bronchial epithelial cell line showed that pyrolytic treatment effectively achieved detoxification of contaminated soil extracts. As expected, TPH and PAH removal efficiencies increased with increasing treatment intensity (i.e., higher temperatures and longer residence times). However, higher treatment intensities decreased soil fertility, suggesting that there is an optimal system-specific intensity for fertility restoration. Overall, this study highlights trade-offs between pyrolytic treatment intensity, hydrocarbon removal efficiency, and fertility restoration while informing the design, optimization, and operation of large-scale pyrolytic systems to efficiently remediate crude-oil-contaminated soils.

Specific ion effects on the aggregation behavior of aquatic natural organic matter.

Abstract: Specific ion effects on the aggregation behavior of a reference aquatic natural organic matter (NOM), Suwannee River NOM (SRNOM), were investigated using kinetic, titration, calorimetric, and surface tension methods. Monovalent cations induced structural compacting of SRNOM, but not its aggregation. Their ability to induce structural compacting follows the order: Cs+ > Rb+ > K+ > Na+ > Li+. Divalent cations except Mg2+ can readily induce SRNOM aggregation. Their critical coagulation concentrations (CCC) follow the order: CCCSr > CCCCa > CCCBa. Electrokinetic, titration, and calorimetric data suggest that monovalent cations have weak interactions with SRNOM, while divalent cations strongly interact with SRNOM. Overall, the cation specificity in aggregation is determined by cation-NOM interactions and their ability to modulate surface tension. Specific ion effects of monovalent cations correlate to their hydration free energy, while that of divalent cations correlate to the ratio of the hydration entropy of cation to the enthalpy change of cation-NOM interactions. The cation specificity is consistent with the extended Derjaguin-Landau-Verwey-Overbeek theory, and the intermolecular interaction energy is dominated by the Lewis acid-base interactions. Our results suggest that specific cations should be targeted to predict or manipulate intermolecular interactions of aquatic NOM in natural and engineered settings.

Bacteriophages from Arsenic-Resistant Bacteria Transduced Resistance Genes, which Changed Arsenic Speciation and Increased Soil Toxicity

Abstract: Lysogenic phages are known to serve as transfer vectors for bacterial genes involved in biotransformation of various environmental pollutants. However, their role in arsenic-contaminated environments is largely undocumented. Here, lysogenic phages were chemically induced (with mitomycin C) in soil samples from two contaminated sites, and arsenic resistance genes arsC (coding for As(V) reduction to excretable (via efflux pumps) but more toxic As(III)) and arsM (coding for As(III) methylation) were detected in these phage genomes. The relative abundance of these genes (per phage particle) was positively correlated with that in the corresponding indigenous soil bacterial communities (resistance gene per 16S rRNA), with R2 = 0.974 for arsC and 0.761 for arsM. Microcosm studies with 100 mg/kg of arsenic soil showed that phages (amended at 5.0 × 107 phages per gram soil) enhanced the propagation of arsC by 122-fold and arsM by 575-fold, relative to unamended soil. This increased the As(III) concentration by 4.3...

Bottom-up biofilm eradication using bacteriophage-loaded magnetic nanocomposites: a computational and experimental study

Abstract: Biofilms cause a variety of pervasive problems in water treatment, distribution and reuse systems that are difficult to mitigate due to their resistance to disinfectants. We used magnetic phage-nanocomposite conjugates (PNCs) to target bacteria in biofilm inner layers for bottom-up eradication. Polyvalent Podoviridae phages PEB1 (54 nm) or PEB2 (86 nm) were covalently conjugated (via amide bonds) with magnetic colloidal nanoparticle clusters (CNCs) of different sizes (150, 250 or 500 nm). Smaller CNCs with higher density of amino groups loaded phages more efficiently than the largest CNCs (e.g., for PEB1, 60 ± 4, 62 ± 5, and 47 ± 4 phages were loaded per μm2). Smaller PNCs dispersed phages more evenly throughout the biofilm bottom, significantly disrupting the biofilm bottom layer and detaching the biofilm within 6 h. The biofilm removal efficiency was 98.3 ± 1.4% for dual species biofilm (i.e., Escherichia coli and Pseudomonas aeruginosa) and 92.2 ± 3.1% for multi-species biofilm (i.e., E. coli, P. aeruginosa, and non-hosts Bacillus subtilis and Shewanella oneidensis). Large PNCs caused higher physical disruption but lower corresponding removal efficiencies (i.e., 80.2 ± 3.4% for dual species biofilm and 67.6 ± 3.8% for multi-species biofilm) due to lower horizontal diffusion at the bottom of the biofilm. A semi-empirical numerical model corroborated the higher biofilm removal efficiency with smaller PNCs and inferred that PNC size influences the mode of phage propagation: Small PNCs facilitate biofilm bottom clearance with significant horizontal dispersion while large PNCs mainly enhance vertical propagation. Overall, this study demonstrates the importance of size control to enhance the biofilm eradication capability of PNCs as an alternative or complementary biofilm control strategy.

Author Correction: Global diversity and biogeography of bacterial communities in wastewater treatment plants

Abstract: In the version of this Article originally published, the name of the author ‘Mathew Robert Brown’ was incorrectly written as ‘Mathew Brown’ in the main author list and as ‘Matthew Brown’ in the Global Water Microbiome Consortium list. In addition, in the Global Water Microbiome Consortium list, the names of the authors ‘Kevin F. Boehnke’, ‘Janeth Sanabria’ and ‘Adalberto Noyola’ were incorrectly written as ‘Kevin Boehnke’, ‘Janeth Sanabria Gomez’ and ‘Adalberto Noyola Robles’, respectively. The names have now been corrected and the author initials in the author contributions section updated accordingly.

Photolysis of graphene oxide in the presence of nitrate: implications for graphene oxide integrity in water and wastewater treatment

Abstract: Despite the widespread use of graphene oxide (GO) in diverse applications and increasing interest in its inclusion in some water treatment devices, mechanistic understanding of photochemical GO transformations is limited. This is an important knowledge gap relevant to GO performance and durability. We examined the reaction pathways and products of a GO suspension under UV irradiation in the presence of nitrate, a common anion in water and wastewater treatment processes. As the nitrate concentration increased, the dominant pathway of GO transformation changed from direct photolysis to indirect photolysis enhanced by the production of hydroxyl radicals (˙OH) during UV irradiation of nitrate. At environmentally relevant concentrations (e.g., 1 mM), nitrate induced significant fragmentation of the GO nanostructure. The significant effects of ˙OH on GO morphology and surface properties were verified by negative-control tests, including deoxygenation of the suspension, reactive oxygen species (ROS) inhibition and radical trapping, and by γ-radiolysis, known to generate a single ROS: ˙OH. Supplemental photolysis experiments conducted using graphite demonstrated that the main reaction pathways of the indirect photolysis of GO not only include the oxidation reactions between ˙OH and the oxidized domains of GO, but also the electrophilic addition reaction between ˙OH and the aromatic domains. These findings have significant implications for GO integrity and durability in systems involving incidental or purposeful exposure to UV irradiation.