Per E. Stromhaug

TL;DR: In this paper, the authors present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macro-autophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.

TL;DR: A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.

TL;DR: This work shows that Atg9 and Atg23 cycle through the pre-autophagosomal structure (PAS) in a process governed by the Atg1-Atg13 signaling complex, and postulates that AtG9 employs a recycling system mechanistically similar to that used at yeast early and late endosomes.

TL;DR: In this paper, a quantitative electron microscopic study confirmed that leupeptin induced a severalfold increase in the number of hepatocytic amphisomes (recognized by their gold particle contents; otherwise, their ultrastructure was quite similar to autophagosomes).

TL;DR: This study elucidates a molecular mechanism for cargo specificity in this autophagy-related trafficking pathway involving four discrete steps and offers insights into physiological processes that are critical in cellular homeostasis, including specific packaging of damaged or superfluous organelles for lysosomal delivery and breakdown.