P
Peter James
Researcher at Lund University
Publications - 184
Citations - 7297
Peter James is an academic researcher from Lund University. The author has contributed to research in topics: Proteome & Peptide sequence. The author has an hindex of 42, co-authored 180 publications receiving 6917 citations. Previous affiliations of Peter James include Uppsala University & Royal Institute of Technology.
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Protein Identification by Mass Profile Fingerprinting
TL;DR: An algorithm for identifying proteins at the sub-microgram level without sequence determination by chemical degradation is developed, providing a rapid and sensitive link between genomic sequences and 2D gel electrophoresis mapping of cellular proteins.
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Methods for the detection and analysis of protein-protein interactions
TL;DR: Four strategies for validation of protein–protein interactions are presented: confocal microscopy for intracellular colocalization of proteins, coimmunoprecipitation, surface plasmon resonance (SPR) and spectroscopic studies.
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Calmodulin-binding domains: just two faced or multi-faceted?
TL;DR: The Ca(2+)-binding protein calmodulin binds to and activates several cellular enzymes in response to a rise in Ca2+ concentration, and the modulation of the binding equilibrium of these helices between intramolecular and intermolecular sites forms a focal point for crosstalk between various signalling pathways.
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Quantitation and Facilitated de Novo Sequencing of Proteins by Isotopic N-Terminal Labeling of Peptides with a Fragmentation-Directing Moiety
TL;DR: The approach is based on the use of an isotopically labeled reagent to quantitate (by mass spectrometry) the ratio of peptides from digests of a protein being expressed under different conditions, and allows quantitation of the changes occurring in spots or bands that contain more than one protein.
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Identification and primary structure of a calmodulin binding domain of the Ca2+ pump of human erythrocytes.
TL;DR: Structural analysis of the single radioactive peak showed that it shares features with the calmodulin binding domains of other enzymes which are regulated by cal modulin.