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Peter W. Andrews

Bio: Peter W. Andrews is an academic researcher from University of Sheffield. The author has contributed to research in topics: Cellular differentiation & Embryonic stem cell. The author has an hindex of 71, co-authored 280 publications receiving 21323 citations. Previous affiliations of Peter W. Andrews include East Sussex County Council & Wistar Institute.


Papers
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Journal ArticleDOI
Oluseun Adewumi1, Behrouz Aflatoonian2, Lars Ährlund-Richter3, Michal Amit4, Peter W. Andrews2, Gemma Beighton5, Paul Bello6, Nissim Benvenisty7, Lorraine S. Berry1, Simon Bevan, Barak Blum7, Justin Brooking8, Kevin G. Chen9, Andre Bh Choo, Gary A. Churchill, Marie Corbel10, Ivan Damjanov11, John S Draper12, Petr Dvorak13, Petr Dvorak14, Katarina Emanuelsson, Roland A. Fleck1, Angela Ford2, Karin Astrid Maria Gertow3, Karin Astrid Maria Gertow6, Marina Gertsenstein12, Paul J. Gokhale2, Rebecca S. Hamilton9, Alex Hampl14, Alex Hampl13, Lyn Healy1, Outi Hovatta3, Johan Hyllner, Marta P. Imreh15, Marta P. Imreh3, Joseph Itskovitz-Eldor4, Jamie P. Jackson2, Jackie Johnson6, Mark Jones2, Kehkooi Kee16, Benjamin L. King, Barbara B. Knowles, Majlinda Lako17, Franck Lebrin18, Barbara S. Mallon9, Daisy Manning19, Yoav Mayshar7, Ronald D.G. McKay9, Anna E. Michalska6, Milla Mikkola20, Masha Mileikovsky12, Stephen L. Minger21, Harry Moore2, Christine L. Mummery, Andras Nagy, Norio Nakatsuji22, Carmel M. O’Brien6, Steve Oh, Cia Olsson20, Timo Otonkoski20, Kye-Yoon Park9, Robert Passier, Hema Patel1, Minal Patel21, Roger A. Pedersen10, Martin F. Pera23, Marian S Piekarczyk19, Renee A. Reijo Pera16, Benjamin Reubinoff, Allan J. Robins, Janet Rossant12, Peter J. Rugg-Gunn12, Peter J. Rugg-Gunn10, Thomas C Schulz, Henrik Semb, Eric S Sherrer, Henrike Siemen16, Glyn Stacey1, Miodrag Stojkovic17, Hirofumi Suemori22, Jin P. Szatkiewicz, Tikva Turetsky, Timo Tuuri20, Steineke van den Brink, Kristina Vintersten12, Sanna Vuoristo20, Dorien Ward, Thomas A Weaver, Lesley Young1, Weidong Zhang 
TL;DR: The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide and found that despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers ofhuman embryonic stem cells.
Abstract: The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

1,064 citations

Journal ArticleDOI
TL;DR: It is suggested that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.
Abstract: We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

1,046 citations

Journal ArticleDOI
TL;DR: Observations constitute the first instance of extensive somatic differentiation of a clonal human EC cell line in vitro when exposed to retinoic acid.

804 citations

Journal ArticleDOI
TL;DR: The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples, and 895 genes were identified that are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.
Abstract: Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.

679 citations

Journal Article
TL;DR: These cell lines derived from teratocarcinoma cell line Tera-2 constitute the first example of clonal human embryonal carcinoma cells, adapted to growth in vitro, that have retained the capacity for differentiation into diverse somatic tissues.

653 citations


Cited by
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Journal ArticleDOI
30 Nov 2007-Cell
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.

18,175 citations

Journal ArticleDOI
06 Nov 1998-Science
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Abstract: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.

15,555 citations

01 Jan 2016
TL;DR: The modern applied statistics with s is universally compatible with any devices to read, and is available in the digital library an online access to it is set as public so you can download it instantly.
Abstract: Thank you very much for downloading modern applied statistics with s. As you may know, people have search hundreds times for their favorite readings like this modern applied statistics with s, but end up in harmful downloads. Rather than reading a good book with a cup of coffee in the afternoon, instead they cope with some harmful virus inside their laptop. modern applied statistics with s is available in our digital library an online access to it is set as public so you can download it instantly. Our digital library saves in multiple countries, allowing you to get the most less latency time to download any of our books like this one. Kindly say, the modern applied statistics with s is universally compatible with any devices to read.

5,249 citations

Journal Article
TL;DR: This research examines the interaction between demand and socioeconomic attributes through Mixed Logit models and the state of art in the field of automatic transport systems in the CityMobil project.
Abstract: 2 1 The innovative transport systems and the CityMobil project 10 1.1 The research questions 10 2 The state of art in the field of automatic transport systems 12 2.1 Case studies and demand studies for innovative transport systems 12 3 The design and implementation of surveys 14 3.1 Definition of experimental design 14 3.2 Questionnaire design and delivery 16 3.3 First analyses on the collected sample 18 4 Calibration of Logit Multionomial demand models 21 4.1 Methodology 21 4.2 Calibration of the “full” model. 22 4.3 Calibration of the “final” model 24 4.4 The demand analysis through the final Multinomial Logit model 25 5 The analysis of interaction between the demand and socioeconomic attributes 31 5.1 Methodology 31 5.2 Application of Mixed Logit models to the demand 31 5.3 Analysis of the interactions between demand and socioeconomic attributes through Mixed Logit models 32 5.4 Mixed Logit model and interaction between age and the demand for the CTS 38 5.5 Demand analysis with Mixed Logit model 39 6 Final analyses and conclusions 45 6.1 Comparison between the results of the analyses 45 6.2 Conclusions 48 6.3 Answers to the research questions and future developments 52

4,784 citations

Journal ArticleDOI
23 Sep 2005-Cell
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.

4,447 citations