Showing papers by "Phillip A. Sharp published in 1979"
TL;DR: It is demonstrated that the expression of many early adenovirus mRNAs is dependent upon the activity of a pre-early viral product, which is defective in adanovirus 5 host range (Ad hr) group I mutants.
642 citations
TL;DR: Cloned in Escherichia coli a DNA copy of mRNA coding for bovine preproparathyroid hormone, which clarifies the hormone's amino acid sequence, which has been disputed.
Abstract: We have cloned in Escherichia coli a DNA copy of mRNA coding for bovine preproparathyroid hormone. Double-stranded DNA was inserted into the Pst I site in plasmid pBR322 by using the poly(dG)-poly(dC) homopolymer extension technique to join the DNA molecules. Recombinant plasmids coding for preproparathyroid hormone were identified by the plasmid's ability to arrest specifically the translation of preproparathyroid hormone mRNA. The nucleotide sequence of the largest recombinant was determined by using both chemical and enzymatic techniques. The parathyroid insert contains 470 nucleotides--102 nucleotides from the 5' noncoding region of the mRNA, 345 nucleotides representing the entire coding region, and 23 nucleotides from the 3' noncoding region. The coding sequence clarifies the hormone's amino acid sequence, which has been disputed. Codon usage is discussed.
88 citations
TL;DR: The structure of steady-state nuclear late adenovirus 2 RNA is consistent with these molecules being intermediates in the intramolecular processing of mRNA from a much longer initial transcript.
75 citations
TL;DR: Nuclei isolated from HeLa cells 16 hours post infection by adenovirus serotype 2 synthesize high levels of viral specific RNA in vitro, and large RNA molecules containing incompletely spliced tripartite leader segments (presumptive processing intermediates) are present in both poly(A) − and poly( a) + RNA.
51 citations
TL;DR: The location of the acquired cellular sequences within the envelope gene was variable in different MSV isolates, suggesting that the cellular sequences can be expressed in different positions relative to murine leukemia virus-derived information present in MSV.
Abstract: The RNA genomes of a variety of murine sarcoma viruses (MSV) were compared by heteroduplex analysis. These viruses included the Moloney-derived isolates 124-MSV, m1-MSV, m3-MSV, HT1-MSV, and NP-MSV and also two independent isolates, Gazdar MSV and 1712-MSV. All of these viral genomes exhibited the acquired cellular sequences previously identified in 3124-MSV and thought to be responsible for transformation and sarcomagenesis. The location of the acquired cellular sequences within the envelope gene was variable in different MSV isolates, suggesting that the cellular sequences can be expressed in different positions relative to murine leukemia virus-derived information present in MSV. Deletions in the gag coding region of the different MSVs were consistent with their known gag-related gene products. Based on several features of the hetero-duplex analysis and the known genealogical relationships of the different MSVs, various possible mechanisms for the formation of MSV are considered.
47 citations
TL;DR: An intracellular subgenomic RNA species from MSV-transformed G8-124 cells was characterized by electron microscopy of RNA:cDNA heteroduplexes using long cDNAs both MSV and MuLV, suggesting a possible role in the expression of 3'-encoded MSV information, possibly including transformation-specific sequences.
27 citations
TL;DR: The complete nucleotide sequence of the capped 5-'terminal undecanucleotide common to these late mRNAs is reported and it should now be possible to locate the major late promotor within the adenoviral genomic DNA sequence.
8 citations
01 Jan 1979
2 citations
01 Jan 1979