Phillip A. Sharp

TL;DR: A method of suppressing a nonsense codon in a gene in a mammalian cell by preparing an oligonucleotide primer having a mismatched anticodon region corresponding to the nonsense codons was proposed in this paper.

TL;DR: The reported antisense strand of the CD19 siRNA used as a negative control in Figure 3 (p. 683) did not have the appropriate sequence, and was therefore not relevant as an unrelated siRNA control.

TL;DR: The murine lymphoid cell line 2017 was engineered to express the green fluorescent protein under the control of an immunoglobulin heavy chain promoter and selected for high activity using multiple rounds of fluorescence-activated cell sorting, demonstrating higher activities in the selected cell lines relative to the parental line and to the non-cell-type-specific histone H2B promoter.

TL;DR: It is established that benzoic acid uptake is pH-dependent (resulting in intracellular acidification) and is enhanced in cells expressing higher levels of MCT1 protein, suggesting that these small organic acids may all be substrates for the same transport pathway.

TL;DR: Equilibrium and kinetic studies of binding of proflavine and ethidium bromide to T2 phage indicate that most of the intraphage DNA is accessible to Proflavine.