Author
Phillip A. Sharp
Other affiliations: McGovern Institute for Brain Research, Medical Research Council, California Institute of Technology ...read more
Bio: Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & RNA splicing. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
Topics: RNA, RNA splicing, Gene, Transcription (biology), DNA
Papers published on a yearly basis
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22 Feb 2001
TL;DR: It is the undersigned's belief that the current National Institutes of Health guidelines, which enable scientists to conduct stem cell research within the rigorous constraints of federal oversight and standards, should remain in effect.
Abstract: We the undersigned urge you to support Federal funding for research using human pluripotent stem cells. We join with other research institutions and patient groups in our belief that the current National Institutes of Health (NIH) guidelines, which enable scientists to conduct stem cell research within the rigorous constraints of federal oversight and standards, should be permitted to remain in effect. The discovery of human pluripotent stem cells is a significant milestone in medical research. Federal support for the enormous creativity of the US biomedical community is essential to translate this discovery into novel therapies for a range of serious and currently intractable diseases.
3 citations
Journal Article•
3 citations
01 Jun 2020
TL;DR: In this paper, the authors used ICP-OES to test the hypothesis that application of zinc-containing fertilisers would increase both the zinc content of, and amount of zinc available for absorption from, maize flour.
Abstract:
Zinc deficiency is prevalent in many low- and middle-income countries. Maize is an important staple food source in many of these regions, however, it contains low levels of nutritionally important minerals including zinc. The purpose of this study was to test the hypothesis that application of zinc-containing fertilisers would increase both the zinc content of, and amount of zinc available for absorption from, maize flour.
Maize was grown in pots in zinc-deficient soils (<1.22 mg/L) at the Bako Agricultural Research Centre, Ethiopia. Zinc (ZnSO4) was applied via several routes: directly to the soil; in the side dressing; as a foliar application; or through seed priming. Zinc content of maize flour samples was measured by ICP-OES. Flour samples were then subjected to simulated in vitro gastrointestinal digestion and zinc uptake by human intestinal Caco-2 cells was measured using ICP-MS.
Addition of zinc to the soil alone did not increase zinc uptake by Caco-2 cells compared with untreated control maize. In contrast, addition of ZnSO4, to the side dressing (3.6-fold increase), through foliar application (2.9-fold increase), or through seed priming (4.2-fold increase) significantly increased zinc uptake compared with untreated controls and the soil zinc treatment groups (1-way ANOVA and Tukey's test; P < 0.05). Zinc uptake by Caco-2 cells was correlated significantly with the zinc content of the flour (r2 = 0.24; P < 0.02).
Together, these data demonstrate that several fertilisation methods can be used to increase zinc content of maize flour. This additional zinc is, at least partly, available for absorption by human intestinal epithelial cells. Agronomic biofortification is therefore a promising strategy to combat zinc deficiency in vulnerable populations.
This work was funded in part by a grant from the Bill & Melinda Gates Foundation.
2 citations
2 citations
01 Jan 1979
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TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
32,946 citations
TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Abstract: The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.
22,269 citations
28 Jul 2005
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。
18,940 citations
TL;DR: The current understanding of miRNA target recognition in animals is outlined and the widespread impact of miRNAs on both the expression and evolution of protein-coding genes is discussed.
18,036 citations
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Abstract: A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
13,805 citations