Author
Phillip A. Sharp
Other affiliations: McGovern Institute for Brain Research, Medical Research Council, California Institute of Technology ...read more
Bio: Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & RNA splicing. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
Topics: RNA, RNA splicing, Gene, Transcription (biology), DNA
Papers published on a yearly basis
Papers
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Journal Article•
1 citations
1 citations
Patent•
30 Sep 2003
TL;DR: In this paper, the authors present systems and methods for selecting and designing siRNAs to effectively inhibit or reduce levels and/or expression of target transcripts, which can be used to identify hypersensitive sites.
Abstract: The present invention provides systems and methods for selecting and designing siRNAs to effectively inhibit or reduce levels and/or expression of target transcripts. In particular, the invention provides computer based systems and methods for implementing rules useful in selecting and designing siRNAs. The invention provides siRNAs selected and/or designed in accordance with the inventive methods. The invention further provides methods for systematically testing siRNAs, thereby permitting identification of hypersensitive sites and preferred siRNAs.
1 citations
01 Jan 1975
TL;DR: It is shown that after infection with SV40, monkey cells undergo a productive or lytic response, during which there is an ordered appearance of virus-specific functions, with some virus products present at all times and others detectable only in the late stages of the viral growth cycle.
Abstract: As long as we take reasonable care, we can grow stocks of Simian Virus that are remarkably homogeneous. Each infectious particle contains a single molecule of DNA weighing 3.4 x 106 daltons—enough to code for perhaps 5–8 polypeptides of average size. The manner in which this virus-coded information is expressed is highly cell-dependent. For example, after infection with SV40, monkey cells undergo a productive or lytic response, during which there is an ordered appearance of virus-specific functions, with some virus products present at all times and others detectable only in the late stages of the viral growth cycle. The infection progresses through a well-defined series of episodes, which culminate in cell death and the concomitant liberation of a new crop of virus particles. The events are multiplicity-independent in that they occur in the same order, albeit at different rates, in cells infected either with one infectious particle or with a hundred. By contrast, the outcome of exposing mouse cells to SV40 is very different.
1 citations
Patent•
23 Jun 2010TL;DR: In this paper, the authors proposed methods for increasing the activity of an inhibitory RNA (RNAi) in a subject requiring administering one or more poly-ADP-ribose polymerase (PARP) inhibitors and/or one OR more PARG activators to the subject.
Abstract: The invention provides methods for increasing the activity of an inhibitory RNA (RNAi) in a subject requiring administering one or more poly-ADP-ribose polymerase (PARP) inhibitors and/or one or more PARG activators to the subject. The invention also provides methods for increasing the activity of an inhibitory RNA in a cell or cell population requiring contacting a cell or cell population with one or more PARP inhibitors and/or one or more PARG activators. The invention further provides compositions and kits containing one or more PARP inhibitors and/or one or more PARG activators.
1 citations
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TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
32,946 citations
TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Abstract: The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.
22,269 citations
28 Jul 2005
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。
18,940 citations
TL;DR: The current understanding of miRNA target recognition in animals is outlined and the widespread impact of miRNAs on both the expression and evolution of protein-coding genes is discussed.
18,036 citations
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Abstract: A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
13,805 citations