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Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Predictive Identification of Exonic Splicing Enhancers in Human Genes
TL;DR: A computational method, RESCUE-ESE, was developed that predicts which sequences have ESE activity by statistical analysis of exon-intron and splice site composition, and identified 10 predicted ESE motifs that enable prediction of the splicing phenotypes of exonic mutations in human genes.
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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
TL;DR: A cell-free system for studying the synthesis of mRNA in mammalian cells, which consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA.
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TAZ, a transcriptional modulator of mesenchymal stem cell differentiation.
Jeong Ho Hong,Eun Sook Hwang,Michael T. McManus,Adam Amsterdam,Yu Tian,Ralitsa Kalmukova,Elisabetta Mueller,Thomas L. Benjamin,Bruce M. Spiegelman,Phillip A. Sharp,Nancy Hopkins,Michael B. Yaffe +11 more
TL;DR: It is reported that a 14-3-3–binding protein, TAZ (transcriptional coactivator with PDZ-binding motif), coactivates Runx2- dependent gene transcription while repressing PPARγ-dependent gene transcription, indicating that TAZ functions as a molecular rheostat that modulates MSC differentiation.
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Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Xuebing Wu,David A. Scott,Andrea J. Kriz,Anthony C. Chiu,Patrick D. Hsu,Daniel B. Dadon,Albert W. Cheng,Alexandro E. Trevino,Silvana Konermann,Sidi Chen,Rudolf Jaenisch,Feng Zhang,Phillip A. Sharp +12 more
TL;DR: A two-state model for Cas9 binding and cleavage is proposed, in which a seed match triggers binding but extensive pairing with target DNA is required for cleavage.
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Five intermediate complexes in transcription initiation by RNA polymerase II
TL;DR: A native gel electrophoresis DNA binding assay was used to resolve complexes formed on the adenovirus Major Late Promoter by general transcription factors and RNA polymerase II, generating new complexes that contained accurately initiated transcripts associated with the transcription machinery and the template DNA.