P
Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Journal ArticleDOI
Embryonic lethality, decreased erythropoiesis, and defective octamer-dependent promoter activation in Oct-1-deficient mice.
TL;DR: A critical role for Oct-1 during development and a stringent gene dosage effect with Oct-2 in mediating postnatal survival are suggested.
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Multiple sets of basal factors initiate transcription by RNA polymerase II.
TL;DR: It is proposed that a significant level of basal transcription only requires a minimal set of factors, and stimulation by upstream activators may in part be mediated by the inclusion of additional basal factors into the initiation reaction.
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Effect of nanoparticle conjugation on gene silencing by RNA interference.
TL;DR: It is shown that the accessibility of the siRNA linked to the nanoparticle and the lability of the cross-linker are critical for efficient gene knockdown.
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Replication of RNA by the DNA-dependent RNA polymerase of phage T7.
TL;DR: The T7 RNA polymerase-X RNA system provides an interesting model for studying replication of RNA by DNA-dependent RNA polymerases, and has been proposed to propagate viroids and hepatitis delta, pathogenic RNAs whose replication seems to depend on cellular RNAs.
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RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex
TL;DR: Proteins purified on the basis of their affinity for RNA polymerase II effectively substitute for previously defined transcription initiation factors and are shown to be required for a DNA conformation change near the initiation site of the promoter.