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Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Journal ArticleDOI
Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.
Richard A. Padgett,Maria M. Konarska,Markus Aebi,Horst Hornig,Charles Weissmann,Phillip A. Sharp +5 more
TL;DR: Mutants of the rabbit beta-globin gene lacking the natural site of branch formation in the second intervening sequence have been analyzed for in vitro splicing activity and the sequences at the branch sites did not conform to the previously determined consensus sequence.
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Global microRNA depletion suppresses tumor angiogenesis
Sidi Chen,Yuan Xue,Xuebing Wu,Cong Le,Arjun Bhutkar,Eric L. Bell,Feng Zhang,Robert Langer,Phillip A. Sharp +8 more
TL;DR: It is shown that depletion of all microRNAs suppresses tumor angiogenesis, and deletion of regions in FIH1 3' untranslated regions (UTRs) that contain microRNA-binding sites are deleted, which derepressesFIH1 protein and represses hypoxia response, suggesting that micro RNAs promote tumor responses to hypoxIA andAngiogenesis by repressing FIH 1.
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Effects of copper on the expression of metal transporters in human intestinal Caco-2 cells
TL;DR: Interestingly, exposure to copper increased iron efflux from Caco‐2 cells and up regulated IREG1 (iron‐regulated mRNA) expression and that DMT1 protein and mRNA expression were decreased following exposure (24 h) to high copper.
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Dynamic and differential regulation of NKCC1 by calcium and cAMP in the native human colonic epithelium
Amy Reynolds,Alyson Parris,Luke A. Evans,Luke A. Evans,Susanne Lindqvist,Phillip A. Sharp,Michael P. Lewis,R Tighe,Mark Williams +8 more
TL;DR: In conclusion, persistent NKCC1 activation by cAMP is constrained by a Ca2+‐dependent cycle of co‐transporter internalization, degradation and re‐expression; this is a novel mechanism to limit intestinal fluid loss.
Journal ArticleDOI
RNA Polymerase II Accumulation in the Promoter-Proximal Region of the Dihydrofolate Reductase and γ-Actin Genes
Chonghui Cheng,Phillip A. Sharp +1 more
TL;DR: The results suggest that the accumulation of paused Pol II in promoter-proximal regions may be a common feature of gene regulation in mammalian cells.