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Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing.
TL;DR: The consensus sequence at the 5' splice site is probably recognized twice during splicing of most introns; however, some pre-mRNAs could potentially be processed in the absence of interactions with U1 snRNP in regions of the nucleus containing high concentrations of SR protein.
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Selection in vitro of novel ribozymes from a partially randomized U2 and U6 snRNA library
TL;DR: The isolation of branching activities by the in vitro selection protocol was unanticipated and was due to surprising properties of reverse transcriptase, which can read through 2′,5′‐ or 2′‐,3′‐branches and efficiently perform non‐templated intramolecular jumps.
RNA Bind-n-Seq: Quantitative Assessment of the Sequence and Structural Binding Specificity of RNA Binding Proteins
Nicole J. Lambert,Alex Robertson,Mohini Jangi,Sean E. McGeary,Phillip A. Sharp,Christopher B. Burge +5 more
TL;DR: RNA Bind-n-Seq (RBNS) as mentioned in this paper is a method that comprehensively characterizes sequence and structural specificity of RNA binding proteins (RBPs), and its application to the developmental alternative splicing factors RBFOX2, CELF1/CUGBP1, and MBNL1.
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Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.
TL;DR: RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis.
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An amber suppressor tRNA gene derived by site-specific mutagenesis: cloning and function in mammalian cells.
TL;DR: Interestingly, the amplification of an amber suppressor gene in CV-1 cells does not interfere with SV40 production, suggesting that suppression of amber codons may not be very deleterious to mammalian cell metabolism.