scispace - formally typeset
Search or ask a question
Author

Pietro Ferraro

Bio: Pietro Ferraro is an academic researcher from National Research Council. The author has contributed to research in topics: Digital holography & Holography. The author has an hindex of 61, co-authored 653 publications receiving 12666 citations. Previous affiliations of Pietro Ferraro include Aeritalia & Centre national de la recherche scientifique.


Papers
More filters
Journal ArticleDOI
TL;DR: An optical configuration in which floating particles in a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast maps is proposed.
Abstract: We propose an optical configuration in which floating particles in a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast maps. The configuration is simply made by two laser beams from the same laser source. One beam provides the optical forces for driving the particle along appropriate paths, but at same time works as the object illumination beam in the holographic microscope. The second beam plays the role of the reference beam, allowing recording of an interference fringe pattern (i.e., the digital hologram) in an out-of-focus image plane. The system and method are illustrated and experimental results are offered for polymeric particles as well as for in vitro cells with the aim to demonstrate the approach.

45 citations

Journal ArticleDOI
TL;DR: In this paper, the authors demonstrate fabrication of periodically poled lithium niobate samples by electric field poling, after patterning by interference lithography, and investigate the poling process at an overpoling regime which caused the appearance of submicron dot domains very similar to those induced by backswitch but different in nature.
Abstract: We demonstrate fabrication of periodically poled lithium niobate samples by electric field poling, after patterning by interference lithography. Furthermore we investigate the poling process at an overpoling regime which caused the appearance of submicron dot domains very similar to those induced by backswitch but different in nature. We show the possibility for realizing submicron-scaled threedimensional domain patterns that could be applied to the construction of photonic crystals and in nonlinear optics. We show that high etch-rate applied to such structures allows to obtain pyramidal-like submicron relief structures which in principle could find application for waveguide construction in photonic bandgap devices.

44 citations

Journal ArticleDOI
TL;DR: A pyro-concentrator able to accumulate biomolecules directly onto a conventional binding surface to open the way to a mass-based technology for sensing molecules at very low concentrations, in environmental as well as in diagnostics applications.
Abstract: Biomolecule detection is important in many fields, but obviously becomes increasingly difficult as concentrations decrease. Here, the authors demonstrate a method to dispense and concentrate biomolecules onto a target slide, greatly improving the detection limits.

44 citations

Journal ArticleDOI
01 Sep 2020
TL;DR: Recent improvements in microfluidics, imaging label‐free diagnosis and cell classification by artificial intelligence and how to combine them to realize an intelligent platform based on lab‐on‐chip technology are surveyed and discussed.
Abstract: Circulating tumor cells (CTCs) are rare tumor cells released from primary, metastatic, or recurrent tumors in the peripheral blood of cancer patients. CTCs isolation from peripheral blood and their molecular characterization represent a new marker in cancer screening, a diagnostic tool called “liquid biopsy” (LB). Compared to traditional tissue biopsy that is invasive and does not reveal tumor heterogeneity, LB is noninvasive and reflects in “real‐time” tumor dynamism and drug sensitivity. In the frame of LB, a new paradigm based on single‐cell and label‐free analysis based on morphological analysis is emerging. Here, we review the latest research developments in this emerging vision of LB. In particular, we survey and discuss recent improvements in microfluidics, imaging label‐free diagnosis and cell classification by artificial intelligence and how to combine them to realize an intelligent platform based on lab‐on‐chip technology. This prospect appears to open up promising and intriguing new scenarios for cancer management through single‐cell analysis that will revolutionize the future of early cancer diagnosis and therapeutic choice with disruptive impact on the society.

44 citations

Journal ArticleDOI
TL;DR: This work investigates the problem of counting and estimating velocity of cells flowing inside a scattering chip and proposes and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect.
Abstract: We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH) In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect This method helps, thanks to DH, to recover information which, otherwise, would be completely lost

44 citations


Cited by
More filters
Journal Article
TL;DR: In this article, a fast Fourier transform method of topography and interferometry is proposed to discriminate between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour generation techniques.
Abstract: A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

3,742 citations

Journal ArticleDOI
TL;DR: In this paper, the authors review the recent developments in the area of optical fiber grating sensors, including quasi-distributed strain sensing using Bragg gratings, systems based on chirped gratings and intragrating sensing concepts.
Abstract: We review the recent developments in the area of optical fiber grating sensors, including quasi-distributed strain sensing using Bragg gratings, systems based on chirped gratings, intragrating sensing concepts, long period-based grating sensors, fiber grating laser-based systems, and interferometric sensor systems based on grating reflectors.

3,665 citations

01 Jan 2006

3,012 citations