Pietro Ferraro

Bio: Pietro Ferraro is an academic researcher from National Research Council. The author has contributed to research in topics: Digital holography & Holography. The author has an hindex of 61, co-authored 653 publications receiving 12666 citations. Previous affiliations of Pietro Ferraro include Aeritalia & Centre national de la recherche scientifique.

Integrated optical instrumentation for the diagnostics of parts by embedded or surface attached optical sensors

Abstract: A system based on integrated optical technologies for the measurement and diagnostics of physical parameters on whatever structure, by the use of optical sensors, made by the fiber embedded Bragg grating method and by the use of a planar integrated optics device for the analysis of the optical signal. The sensors may be embedded or bonded to the structure, allowing the measurement of parameters like strain and temperature, in either a static or dynamic regime. The system pertains to the technical field of the diagnostics and measurements of mechanical or thermal parameters and to the application field of ground, water and aerospace transportation and also to the application field of construction.

Angular spectrum method with correction of anamorphism for numerical reconstruction of digital holograms on tilted planes

Abstract: We present a new method for numerically reconstructing digital holograms on tilted planes. The method is based on the angular spectrum of plane waves. Fast Fourier transform algorithm is used twice and coordinate rotation in the Fourier domain enables to reconstruct the object field on the tilted planes. Correction of the anamorphism resulting from the coordinate transformation is performed by suitable interpolation of the spectral data. Experimental results are presented to demonstrate the method for a singleaxis rotation. The algorithm is especially useful for tomographic image reconstruction.

3D morphometry of red blood cells by digital holography.

Abstract: Three dimensional (3D) morphometric analysis of flowing and not-adherent cells is an important aspect for diagnostic purposes. However, diagnostics tools need to be quantitative, label-free and, as much as possible, accurate. Recently, a simple holographic approach, based on shape from silhouette algorithm, has been demonstrated for accurate calculation of cells biovolume and displaying their 3D shapes. Such approach has been adopted in combination with holographic optical tweezers and successfully applied to cells with convex shape. Nevertheless, unfortunately, the method fails in case of specimen with concave surfaces. Here, we propose an effective approach to achieve correct 3D shape measurement that can be extended in case of cells having concave surfaces, thus overcoming the limit of the previous technique. We prove the new procedure for healthy red blood cells (RBCs) (i.e., discocytes) having a concave surface in their central region. Comparative analysis of experimental results with a theoretical 3D geometrical model of RBC is discussed in order to evaluate accuracy of the proposed approach. Finally, we show that the method can be also useful to classify, in terms of morphology, different varieties of RBCs.

A skin-over-liquid platform with compliant microbumps actuated by pyro-EHD pressure

Abstract: The unique deformability and the compliance ability of thin sheets on soft substrates attract much interest for studying the phenomena related to elastic instabilities as well as for sensing very weak forces such as those generated by live cells in vitro. However, the techniques used currently for producing such platforms are affected by a high degree of complexity and poor repeatability. Moreover, their deformability is usually used as a passive response to the action of an external force. Herein we propose a novel concept for a reliable and dynamic skin-over-liquid system made of a periodic array of highly compliant microbumps actuated through electrode-free electrohydrodynamic (EHD) pressure. We demonstrate that these structures are highly repeatable and capable of swelling and deflating easily under a simple thermal stimulation driven by the pyroelectric effect, thus providing a challenging platform that can be actively controlled at the microscale. Furthermore, we show the proof of principle by swelling these microbumps for mechanically stimulating live cells in vitro, thus opening the route to more reliable and easy to accomplish assays in the field of mechanobiology. Researchers curious about how mechanical stress impacts cell growth can now turn to silicone plastics that grow microbumps on demand. Simonetta Grilli from Italy’s Institute of Applied Sciences and Intelligent Systems in Pozzuoli and colleagues created a controllable ‘skin-over-liquid’ system by coating a silicone polymer solution onto lithium niobate, a crystal that contains patterned regions of electric charge. An initial plasma treatment produced a flat stiff film on top of the liquid silicone. Thermal heating caused the skin to swell and form dimpled, air mattress-like patterns, corresponding to the underlying lithium niobate domains. Removal of heat restored the sheet to its initial state. The chemically inert skin supported live fibroblast cells and was used to determine reactions to mechanical stress. Significant variations in nuclei and cytoskeletons were observed between cells grown under flat or swollen-skin conditions. We propose an innovative skin-over-liquid system made of a periodic array of highly compliant microbumps actuated through an electrode-free electrohydrodynamic (EHD) pressure. We demonstrate that these structures are highly repeatable and are capable to swell and deflate easily under a simple thermal stimulation driven by pyroelectric effect, thus providing a challenging platform that can be actively controlled at microscale. We show the proof of principle by swelling these microbumps for stimulating mechanically live cells in vitro, thus opening the route to more reliable and easy to accomplish assays in the field of mechanobiology.

Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry

Abstract: A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

Fiber grating sensors

Abstract: We review the recent developments in the area of optical fiber grating sensors, including quasi-distributed strain sensing using Bragg gratings, systems based on chirped gratings, intragrating sensing concepts, long period-based grating sensors, fiber grating laser-based systems, and interferometric sensor systems based on grating reflectors.