Pradeep Kumar Kamila

Bio: Pradeep Kumar Kamila is an academic researcher from Central Tuber Crops Research Institute. The author has contributed to research in topics: Essential oil & Cryptocarya. The author has an hindex of 3, co-authored 10 publications receiving 23 citations.

Chemical Composition, Antioxidant, Anti-inflammatory and Anticancer Activities of Bark Essential Oil of Cryptocarya amygdalina from India

Abstract: Several species of Cryptocarya (Lauraceae) are traditionally used in folk medicines for the treatment of various diseases and disorders in tropical and subtropical countries. The essential oil extr...

Effect of Auxins on Rooting of Stem Cuttings in Hypericum gaitii

Abstract: An efficient vegetative propagation method was developed for Hypericum gaitii. Indole-3-butyric acid (IBA), indole acetic acid, and naphthalene acetic acid in different concentrations and combinati...

Ultrasonic acoustic-cavitation as a novel and emerging energy efficient technique for oil extraction from kapok seeds

Abstract: Ultrasound is one of the emerging and novel techniques in oil extraction process. In this study, the oil was extracted from kapok seed using ultrasonic acoustic cavitation and conventional extraction techniques. The present study results found that Ultrasonic Cavitation-Assisted Extraction (UCAE) produced 92.29% of oil recovery with a shorter extraction time of 10 min compared to solvent extraction (5.7 h) and Soxhlet (8 h). The fatty acid composition of oils revealed that the linoleic acid (33.86%–37.64%), palmitic acid (21.79%–25.42%), and oleic acid (23.39%–25.01%) are predominantly present in oils. The oil obtained from UCAE, contains slightly higher composition of oleic, linoleic, malvalic and sterculic acids compared to other methods. The total energy consumption taking into account all possible utilisation was measured and found that the energy consumption of CUAE method is 80 folds lower than soxhlet and 50 folds lesser than SE. Industrial Relevance This research paper provides KSO production with the high quality, shorter time as well as the energy utilised by ultrasound process seems to be better. This research helps to develop a new and economical method of kapok seed oil production.

Fatty Acid Composition, Phytochemistry, Antioxidant Activity on Seed Coat and Kernel of Paeonia ostii from Main Geographic Production Areas

Abstract: Paeonia ostii is an important woody oil plant cultivated in China on a large scale. Its seed oil is enriched with unsaturated fatty acids and a high content of alpha-linolenic acid (ALA), which are beneficial to human health. The aim of this research is to determine the qualitative traits characteristic of P. ostii seed from various production areas in China. In this study, seed quality traits were evaluated on the basis of proximate composition, content of fatty acids, tocopherol, secondary metabolites, and the antioxidant activity of seed coat (PSC) and kernel (PSK). A high content of total fatty acids (298.89–399.34 mg g−1), crude protein (16.91%–22.73%), and total tocopherols (167.83–276.70 μg g−1) were obtained from PSK. Significant differences were found in the content of palmitic acids (11.31–14.27 mg g−1), stearic acids (2.42–4.24 mg g−1), oleic acids (111.25–157.63 mg g−1), linoleic acids (54.39–83.59 mg g−1), and ALA (99.85–144.71 mg g−1) in the 11 main production areas. Eight and seventeen compounds were detected in PSC and PSK, respectively. A significantly higher content of total phenols was observed in PSC (139.49 mg g−1) compared with PSK (3.04 mg g−1), which was positively related to antioxidant activity. This study indicates that seeds of P. ostii would be a good source of valuable oil and provides a basis for seed quality evaluation for the production of edible oil and potential ALA supplements from the promising woody oil plant.

Effect of different extraction techniques on total phenolic and flavonoid contents, and antioxidant activity of betelvine and quantification of its phenolic constituents by validated HPTLC method

Abstract: The objective of the study is to analyze the effect of different extracting methods on the polyphenolic content and antioxidant activities in Piper betle leaves. In the present research, P. betle leaf extract was prepared by sonication, Soxhlet and maceration methods using acetone (100%, v/v). The efficiency of the extraction methods was estimated by quantifying the total phenolic content (TPC) by the Folin–Ciocalteu method and total flavonoid content (TFC) by AlCl3 colorometric methods, and antioxidant power of the various extracts was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assay. Thin-layer chromatography (TLC) bioautography was carried out to identify antioxidants, and their amount was determined by the newly developed high-performance thin-layer chromatography (HPTLC) method. DPPH free radical scavenging capacity of the different extracts from strongest to weakest was as follows: ascorbic acid (4.27 µg/mL) > sonication (5.35 µg/mL) >, maceration (5.53 µg/mL)>, soxhlet extraction (5.83 µg/mL). Same trend was also observed for the ABTS radical scavenging capacity. Similarly, findings of this study also showed that sonication extract possessed highest phenolic and flavonoid contents followed by maceration and Soxhlet extraction. In addition, important bioactive phenolic constituents which contribute largely towards antioxidant potential such as eugenol and eugenol acetate were quantified using HPTLC (high-performance thin-layer chromatography) method. The average percent recovery of eugenol and eugenol acetate was found to be 97.28% and 98.04%, respectively. The LOD (limit of detection) and LOQ (limit of quantification) for eugenol were 5 and 15 ng/spot, whereas that of eugenol acetate were 10 and 30 ng/spot. The HPTLC densitometric determination also supported the results of antioxidant assays by revealing the presence of higher amount of identified antioxidants in sonication followed by maceration and Soxhlet extraction. The developed HPTLC chromatogram profile may be used as a reference for the standardization of P. betle leaf extracts.