Pubali Dhar

Bio: Pubali Dhar is an academic researcher from University of Calcutta. The author has contributed to research in topics: Lipid peroxidation & Medicine. The author has an hindex of 16, co-authored 48 publications receiving 777 citations. Previous affiliations of Pubali Dhar include Krishnagar Government College.

Dietary effects of conjugated octadecatrienoic fatty acid (9 cis, 11 trans, 13 trans) levels on blood lipids and nonenzymatic in vitro lipid peroxidation in rats.

Abstract: The present study examined the antioxidant activity of conjugated octadecatrienoic fatty acid (9 cis,11 trans,13 trans-18:3), alpha-eleostearic acid, of karela seed (Momordica charantia), fed to rats for 4 wk. The growth pattern of rats and the effect on plasma cholesterol and high density lipoprotein (HDL) cholesterol and peroxidation of plasma lipid, lipoprotein, eryhrocyte membrane, and liver lipid were measured. Rats were raised on diets containing sunflower oil mixed with three different levels of conjugated trienoic fatty acid (9c,11t,3t-18:3) 0.5, 2, and 10% by weight; the control group was raised with sunflower oil as dietary oil as the source of linoleic acid (9c,12c-18:2). The growth pattern of the three experimental groups of rats showed no significant difference compared to the control group of rats, but the group with 10% 9c,11t,13t-18:3 had slightly higher body weight than the control group of rats. Concentrations of total cholesterol, HDL-cholesterol, and non-HDL-cholesterol in plasma were similar in all four groups. Plasma lipid peroxidation was significantly lower in the case of 0.5% 9c,11t,13t-18:3 group than the control group and the 2 and 10% 9c,11t,13t-18:3 dietary groups as well. Lipoprotein oxidation susceptibility test with 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups was significantly less susceptible to lipoprotein peroxidation when compared with sunflower oil dietary group, and the dietary group with 0.5% 9c,11t,13t-18:3 showed least susceptibility. There was significant lowering in erythrocyte ghost membrane lipid peroxidation in the 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups compared to the sunflower oil groups. Nonenzymatic liver tissue lipid peroxidation was significantly lower in the group of rats raised on 0.5% 9c,11t,13t-18:3, but the groups on 2 and 10% 9c,11t,13t-18:3 acid did not show any significant difference compared with the control group of rats.

Lutein Content and in Vitro Antioxidant Activity of Different Cultivars of Indian Marigold Flower (Tagetes patula L.) Extracts

Abstract: Three different cultivars of marigold flowers ( Tagetes patula L.) (marigold orange, marigold yellow, and marigold red) were analyzed for the lutein ester contents, and the in vitro antioxidative activities of the flower extracts were compared. The total antioxidant capacity, reducing power, hydroxyl, DPPH, and ABTS(*+) radical scavenging activities, iron chelation capacity, and inhibition of lipid peroxidation in a linoleic acid emulsion system were measured. Iron-mediated Fenton reaction was carried out to evaluate the protective effect of leutin against DNA damage. The marigold orange (MGO) variety contains the maximum amount of lutein. It also had the highest DPPH radical scavenging activity and ABTS radical scavenging activity, with an EC(50) value of 0.344 mg/mL. It was also the most effective against lipid peroxidation and hydroxyl radical scavenging activities. The MGO extract has the maximum reducing power. Hepatic cell damage in iron-mediated Fenton reaction caused by free radicals was reduced by the marigold extracts. Marigold flowers of Indian variety can be effectively utilized to produce lutein ester, which can be used as a food supplement or as an accessible source of natural antioxidants.

Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans

Abstract: Seven Sesamum indicum (sesame) honey samples were collected from Hooghly district of West Bengal, India and analyzed for polyphenol and flavonoid content along with their in vitro free radical scavenging activities. Antibacterial activity and stimulatory effect on multiplication of probiotic bacteria were evaluated. Antioxidant markers like IC50 value for DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) of sesame honey were positively correlated to its polyphenolic content (28.9 ± 0.6 mg GAE/100 g) and color intensity (r ranges between 0.872 and 0.931). Four flavonoids viz., apigenin, quercetin, myricetin, rutin have been identified along with one cinnamic acid derivative (ferulic acid) and two sesame lignans (sesamin and episesamin) by High performance liquid chromatography which can be used as a tool for authentication of sesame honey. Antibacterial activity of sesame honey against some enteropathogenic bacteria Escherichia coli, Vibrio cholerae, S. Typhi, S. Typhimurium were studied. Minimum inhibitory concentration was found to be lowest against S. Typhi (12.5% w/v) and S. Typhimurium (12.5% w/v). Plasmid DNA degradation by sesame honey administration evinced its molecular level of action. Interestingly, it was also found that sesame honey exhibited significant growth promoting property of probiotic strains such as Lactobacillus acidophilus and Bifidobacterium bifidum.

In vitro antioxidant properties of rutin

Abstract: Much work has been carried out in recent years on the beneficial effect of phenolic compounds which act as natural antioxidants and help to neutralize free radicals. We analysed the antioxidant activity of the rutin (quercetin-3-rhamnosyl glucoside) using different assays including: total antioxidant activity and reducing power, hydroxyl radical scavenging assay, superoxide radical scavenging assay, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and lipid peroxidation assay which uses egg yolk as the lipid-rich source. Total antioxidant capacity was determined by the assay based on the decrease in absorbance of β-carotene by the sample. Rutin exhibited strong DPPH radical scavenging activity. At the concentration of 0.05 mg/ml, ascorbic acid (Vc), butylated hydroxytoluene (BHT) and rutin showed 92.8%, 58.8%, and 90.4% inhibition, respectively. In addition, rutin had effective inhibition of lipid peroxidation. Those various antioxidant activities were compared to standard antioxidants such as BHT and Vc.

An overview: biomolecules from microalgae for animal feed and aquaculture

Abstract: Despite being more popular for biofuel, microalgae have gained a lot of attention as a source of biomolecules and biomass for feed purposes. Algae farming can be established using land as well as sea and strategies can be designed in order to gain the products of specific interest in the optimal way. A general overview of the contributions of Algae to meet the requirements of nutrients in animal/aquaculture feed is presented in this study. In addition to its applications in animal/aquaculture feed, algae can produce a number of biomolecules including astaxanthin, lutein, beta-carotene, chlorophyll, phycobiliprotein, Polyunsaturated Fatty Acids (PUFAs), beta-1,3-glucan, and pharmaceutical and nutraceutical compounds which have been reviewed with respect to their commercial importance and current status. The review is further extended to highlight the adequate utilization of value added products in the feeds for livestock, poultry and aquaculture (with emphasis in shrimp farming).