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Pyo-Jam Park

Bio: Pyo-Jam Park is an academic researcher from Konkuk University. The author has contributed to research in topics: Antioxidant & DPPH. The author has an hindex of 38, co-authored 176 publications receiving 7130 citations. Previous affiliations of Pyo-Jam Park include Memorial University of Newfoundland & Chonnam National University.


Papers
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Journal ArticleDOI
TL;DR: In this paper, the antibacterial effect of three kinds of chitooligosaccharides with relatively higher molecular weights (HMWCOS), medium molecular weight (MMWCOS), and lower molecular weight, respectively, was evaluated against various microorganisms.

643 citations

Journal ArticleDOI
TL;DR: Alaska pollack frame protein hydrolysate (APH) was fractionated according to the molecular basis into five major types of APH-I (30, 10, 5, 3, and 1 kDa) using an ultrafiltration (UF) membrane bioreactor system as discussed by the authors.

464 citations

Journal ArticleDOI
TL;DR: In order to utilize yellowfin sole (Limanda aspera) frame protein (YFP), which is normally discarded as industrial waste in the process of fish manufacture, YFPHs were fractionated using an ultra-filtration (UF) membrane system following hydrolysis with pepsin and mackerel intestines crude enzyme (MICE) as mentioned in this paper.
Abstract: In order to utilize yellowfin sole ( Limanda aspera) frame protein (YFP), which is normally discarded as industrial waste in the process of fish manufacture, yellowfin sole frame protein hydrolysates (YFPHs) were fractionated using an ultrafiltration (UF) membrane system following hydrolysis with pepsin and mackerel intestines crude enzyme (MICE). The YFPHs were separated into five major types, YFPH-I (30–10 kDa), YFPH-II (10–5 kDa), YFPH-III (5–3 kDa), YFPH-IV (3–1 kDa), and YFPH-V (below 1 kDa) by using UF membranes with molecular weight cut-offs of 30, 10, 5, 3, and 1 kDa, respectively. The antioxidative activity of the YFPHs was investigated and compared with that of a natural antioxidant, α-tocopherol, used as a reference. Furthermore, the fraction showing strong antioxidative activity was isolated from the YFPHs using consecutive chromatographic methods on an SP-Sephadex C-25 column, on a Sephadex G-75 column, and high-performance liquid chromatography (HPLC) on an octadecylsilane column. The molecular mass of the antioxidant was identified as 13 kDa using HPLC on a gel permeation chromatography (GPC) column, and the antioxidative peptide was composed of 10 N-terminal amino acid residues, RPDFDLEPPY.

350 citations

Journal ArticleDOI
TL;DR: The protein extracted from lecithin-free egg yolk was hydrolyzed with the aid of Alcalase, a commercial enzyme, and two different peptides showing strong antioxidative activity were isolated from the hydrolysates by using consecutive chromatographic methods.
Abstract: The protein extracted from lecithin-free egg yolk, normally discarded by lecithin processing plants, was hydrolyzed with the aid of Alcalase, a commercial enzyme. The hydrolysate was separated through a series of ultrafiltration membranes with molecular weight cutoffs of 10, 5, and 1 kDa; and three types of permeates including 10 K (permeate from 10 kDa), 5 K (permeate from 5 kDa), and 1 K (permeate from 1 kDa) were obtained. The antioxidative efficacy of hydrolysates so obtained was investigated and compared with α-tocopherol. Furthermore, two different peptides showing strong antioxidative activity were isolated from the hydrolysates by using consecutive chromatographic methods including ion exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-25 column, and high-performance liquid chromatography on an octadecylsilane column. The purity of the peptides was identified using capillary electrophoresis. The isolated peptides were composed of 10 and 15 amino acid residues, and both contained a leucine residue at their N-terminal positions.

339 citations

Journal ArticleDOI
TL;DR: In this paper, three kinds of partially deacetylated hetero-chitosans (90, 75 and 50% chitosan) were prepared, and their scavenging activities were investigated against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, alkyl radical, hydroxyl radical and superoxide radical using electron spin resonance spectrometer.

322 citations


Cited by
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Journal ArticleDOI
TL;DR: The chemical structure and relevant biological properties of chitosan for regenerative medicine have been summarized as well as the methods for the preparation of controlled drug release devices and their applications.

2,312 citations

Journal ArticleDOI
TL;DR: The paper reviews the current trend of investigation on antimicrobial activities of chitosan and its mode of action and different physical states are comparatively discussed.

2,260 citations

Journal ArticleDOI
TL;DR: The preparation and properties of innovative chitosan-based biomaterials, with respect to their future applications, are highlighted, with a special focus on wound healing application.

1,694 citations

Journal ArticleDOI
TL;DR: Several selected pharmaceutical and biomedical applications are presented, in which chitin and chitosan are recognized as new biomaterials taking advantage of their biocompatibility and biodegradability.
Abstract: This review describes the most common methods for recovery of chitin from marine organisms. In depth, both enzymatic and chemical treatments for the step of deproteinization are compared, as well as different conditions for demineralization. The conditions of chitosan preparation are also discussed, since they significantly impact the synthesis of chitosan with varying degree of acetylation (DA) and molecular weight (MW). In addition, the main characterization techniques applied for chitin and chitosan are recalled, pointing out the role of their solubility in relation with the chemical structure (mainly the acetyl group distribution along the backbone). Biological activities are also presented, such as: antibacterial, antifungal, antitumor and antioxidant. Interestingly, the relationship between chemical structure and biological activity is demonstrated for chitosan molecules with different DA and MW and homogeneous distribution of acetyl groups for the first time. In the end, several selected pharmaceutical and biomedical applications are presented, in which chitin and chitosan are recognized as new biomaterials taking advantage of their biocompatibility and biodegradability.

1,554 citations

Journal ArticleDOI
TL;DR: The present work is a compilation of recent information on collagen and gelatin extraction from new sources, as well as new processing conditions and potential novel or improved applications, many of which are largely based on induced cross-linking, blending with other biopolymers or enzymatic hydrolysis.

1,496 citations