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Author

Qinhong Wang

Other affiliations: University of Oklahoma
Bio: Qinhong Wang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Medicine & Saccharomyces cerevisiae. The author has an hindex of 20, co-authored 78 publications receiving 1070 citations. Previous affiliations of Qinhong Wang include University of Oklahoma.


Papers
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Journal ArticleDOI
TL;DR: Findings from comprehensive evaluation of untargeted metabolomics software would help guide future improvements of these widely used bioinformatics tools and enable users to properly interpret their metabolomics results.

92 citations

Journal ArticleDOI
TL;DR: Fermentation experiments demonstrated that glucose in the Chlorella biomass hydrolysates was converted into ethanol by Saccharomyces cerevisiae with a yield of 0.47 g g(-1), which is 91% of the theoretical yield, thus a novel route for the hydrolysis of biomass to generate fermentable sugars.

80 citations

Journal ArticleDOI
TL;DR: The authors' proteomic characterization of the industrial strain led to comprehensive understanding of the molecular basis of thermotolerance, which would facilitate future improvement in the industrially important trait of S. cerevisiae by rational engineering.

55 citations

Journal ArticleDOI
TL;DR: An ultra-high throughput screening pipeline for lipase-producing strains based on fluorescence-activated droplet sorting (FADS) using a compact optical system that could be easily set up in an alignment-free manner is developed.
Abstract: Lipases are ubiquitous enzymes of great physiological significance that have been used extensively in multiple industries. Environmental microorganisms are a major source for the discovery of novel lipases with high catalytic efficiency and selectivity. However, current plate-based screening of lipase-producing strains is time consuming, labour intensive and inefficient. In this study, we developed an ultra-high throughput screening pipeline for lipase-producing strains based on fluorescence-activated droplet sorting (FADS) using a compact optical system that could be easily set up in an alignment-free manner. The pipeline includes droplet generation, droplet incubation, picoinjection of the fluorescence probe, and sorting of droplets with a throughput of 2 × 106 drops per h. We applied the pipeline to screen samples collected from different locations, including sediments from a hot spring in Tibet, soils from the Zoige wetland, contaminated soils from an abandoned oilfield, and a Chinese Daqu starter. In total, we obtained 47 lipase-producing bacterial strains belonging to seven genera, including Staphylococcus, Bacillus, Enterobacter, Serratia, Prolinoborus, Acinetobacter, and Leclercia. We believe that this FADS-based pipeline could be extended to screen various enzymes from the environment, and may find wide applications in breeding of industrial microorganisms.

52 citations

Journal ArticleDOI
TL;DR: The work reveals the potential for producing high-performance rhamnolipids from renewable resources on a large scale by selecting suitable substrates and optimizing the fermentation process.
Abstract: To be competitive with common chemical surfactants, the cost of rhamnolipid production must be minimized by selecting suitable substrates and optimizing the fermentation process. With different plant oils as substrates, Pseudomonas aeruginosa TIB-R02 can produce rhamnolipids with different structural characteristics that were confirmed by HPLC/MS analysis. Different rhamnolipids had different performances in interfacial tension. The production of rhamnolipid was greatly enhanced by fermentation optimization with palm oil as substrate. A fermentation-defoaming tandem system was developed to resolve the problems of foaming and medium overflow during scale-up. Finally, the titer of rhamnolipid reached 60 g/l and the yield reached 80 % in a 300 l fermentation-defoaming tandem system. The work reveals the potential for producing high-performance rhamnolipids from renewable resources on a large scale.

47 citations


Cited by
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01 Jan 2011
TL;DR: The sheer volume and scope of data posed by this flood of data pose a significant challenge to the development of efficient and intuitive visualization tools able to scale to very large data sets and to flexibly integrate multiple data types, including clinical data.
Abstract: Rapid improvements in sequencing and array-based platforms are resulting in a flood of diverse genome-wide data, including data from exome and whole-genome sequencing, epigenetic surveys, expression profiling of coding and noncoding RNAs, single nucleotide polymorphism (SNP) and copy number profiling, and functional assays. Analysis of these large, diverse data sets holds the promise of a more comprehensive understanding of the genome and its relation to human disease. Experienced and knowledgeable human review is an essential component of this process, complementing computational approaches. This calls for efficient and intuitive visualization tools able to scale to very large data sets and to flexibly integrate multiple data types, including clinical data. However, the sheer volume and scope of data pose a significant challenge to the development of such tools.

2,187 citations

Journal ArticleDOI
TL;DR: This perspective describes research opportunities and challenges ahead for this new field of research, which holds significant promise towards a biorefinery concept wherein polysaccharides and lignin are treated as equally valuable feedstocks.

483 citations

01 Jan 1979
TL;DR: In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.
Abstract: Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt. Related S. cerevisiae strains show marked alterations in the size of the restriction fragments containing these repetitive DNAs. "Ty1" is one such family of repeated sequences in yeast and consists of a 5.6 kilobase (kb) sequence including a noninverted 0.25 kb sequence of another repetitious family, "delta", on each end. There are about 35 copies of Ty1 and at least 100 copies of delta (not always associated with Ty1) in the haploid genome. A few Ty1 elements are tandem and/or circular, but most are disperse and show (along with delta) some sequence divergence between repeat units. Sequence alterations involving Ty1 elements have been found during the continual propagation of a single yeast clone over the course of a month. One region with a large number of delta sequences (SUP4) also shows a high frequency of sequence alterations when different strains are compared. One of the differences between two such strains involves the presence or absence of a Ty1 element. The novel joint is at one inverted pair of delta sequences.

385 citations

Journal ArticleDOI
TL;DR: Various aspects of three commonly used pretreatment methods, viz., steam explosion, acid and alkaline, applied on various lignocellulosic biomasses to augment their digestibility alongwith the challenges associated with their processing are discussed.

375 citations