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R.A. Borer

Bio: R.A. Borer is an academic researcher from ETH Zurich. The author has contributed to research in topics: Preribosomal RNA & Nucleolus. The author has an hindex of 1, co-authored 1 publications receiving 1056 citations.

Papers
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Journal ArticleDOI
10 Feb 1989-Cell
TL;DR: These unexpected results suggest a role for these major nucleolar proteins in the nucleocytoplasmic transport of ribosomal components and suggest that transient exposure of shuttling proteins to the cy toplasm may provide a mechanism for cytop lasmic regulation of nuclear activities.

1,082 citations


Cited by
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Journal ArticleDOI
04 Mar 1994-Science
TL;DR: In the predicted hybrid protein, the amino terminus of nucleophosmin (NPM) is linked to the catalytic domain of anaplastic lymphoma kinase (ALK), and unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas.
Abstract: The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23. In the predicted hybrid protein, the amino terminus of nucleophosmin (NPM) is linked to the catalytic domain of anaplastic lymphoma kinase (ALK). Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas.

2,260 citations

Journal ArticleDOI
TL;DR: Cytoplasmic NPM is a characteristic feature of a large subgroup of patients with AML who have a normal karyotype, NPM gene mutations, and responsiveness to induction chemotherapy.
Abstract: Background Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein. Methods We used immunohistochemical methods to study the subcellular localization of NPM in bone marrow–biopsy specimens from 591 patients with primary acute myelogenous leukemia (AML). We then correlated the presence of cytoplasmic NPM with clinical and biologic features of the disease. Results Cytoplasmic NPM was detected in 208 (35.2 percent) of the 591 specimens from patients with primary AML but not in 135 secondary AML specimens or in 980 hematopoietic or extrahematopoietic neoplasms other than AML. It was associated with a wide spectrum of morphologic subtypes of the disease, a normal karyotype, and responsiveness to induction chemotherapy, but not with recurrent genetic abnormalities. There was a high frequency of FLT3 internal tandem duplications and absen...

1,718 citations

Journal ArticleDOI
11 Aug 1995-Cell
TL;DR: The heat-stable inhibitor of cAPK contains a nuclear export signal that triggers rapid, active net extrusion of the C-PKl complex from the nucleus, and this NES (residues 35-49), fused or conjugated to heterologous proteins, was sufficient for rapid nuclear export.

1,180 citations

Journal ArticleDOI
15 May 1999-Blood
TL;DR: The elucidation of the molecular basis of acute promyelocytic leukemia emerged as a paradigm for the connection between the bench and bedside and it became apparent that APL was, among the forms of acute myeloid leukemia, uniquely sensitive to all

1,022 citations

Journal ArticleDOI
24 Apr 1997-Nature
TL;DR: Current understanding of nucleocytoplasmic transport is summarized and the importance of regulated transport for signal transduction is illustrated.
Abstract: In eukaryotic organisms, DNA replication and RNA biogenesis occur in the cell nucleus, whereas protein synthesis occurs in the cytoplasm. Integration of these activities depends on selective transport of proteins and ribonucleoprotein particles between the two compartments. Transport across the nuclear envelope occurs through large multiprotein structures, termed nuclear pore complexes. It is signal-mediated and requires both energy and soluble factors, including shuttling carriers. Here I summarize current understanding of nucleocytoplasmic transport and illustrate the importance of regulated transport for signal transduction.

1,016 citations