R.D. Evans

Bio: R.D. Evans is an academic researcher from Duke University. The author has contributed to research in topics: Digital microfluidics & Lab-on-a-chip. The author has an hindex of 6, co-authored 6 publications receiving 770 citations.

Chemical and Biological Applications of Digital-Microfluidic Devices

Abstract: Digital-microfluidic lab-on-a chip (LoC) technology offers a platform for developing diagnostic applications with the advantages of portability, sample and reagent volume reduction, faster analysis, increased automation, low power consumption, compatibility with mass manufacturing, and high throughput. In addition to diagnostics, digital microfluidics is finding use in airborne chemical detection, DNA sequencing by synthesis, and tissue engineering. In this article, we review efforts to develop various LoC applications using electrowetting-based digital microfluidics. We describe these applications, their implementation, and associated design issues.

A scaling model for electrowetting-on-dielectric microfluidic actuators

Abstract: A hydrodynamic scaling model of droplet actuation in an electrowetting-on-dielectric (EWD) actuator is presented that takes into account the effects of contact angle hysteresis, drag from the filler fluid, drag from the solid walls, and change in the actuation force while a droplet traverses a neighboring electrode. Based on this model, the threshold voltage, VT, for droplet actuation is estimated as a function of the filler medium of a scaled device. It is shown that scaling models of droplet splitting and liquid dispensing all show a similar scaling dependence on [t/er(d/L)]1/2, where t is insulator thickness and d/L is the aspect ratio of the device. It is also determined that reliable operation of a EWD actuator is possible as long as the device is operated within the limits of the Lippmann–Young equation. The upper limit on applied voltage, Vsat, corresponds to contact-angle saturation. The minimum 3-electrode splitting voltages as a function of aspect ratio d/L < 1 for an oil medium are less than Vsat. However, for an air medium the minimum voltage for 3-electrode droplet splitting exceeds Vsat for d/L ≥ 0.4. EWD actuators were fabricated to operate with droplets down to 35pl. Reasonable scaling results were achieved.

Integrated Optical Sensor in a Digital Microfluidic Platform

Abstract: The advent of digital microfluidic lab-on-a-chip (LoC) technology offers a platform for developing diagnostic applications with the advantages of portability, increased automation, low-power consumption, compatibility with mass manufacturing, and high throughput. However, most digital microfluidic platforms incorporate limited optical capabilities (e.g., optical transmission) for integrated sensing, because more complex optical functions are difficult to integrate into the digital microfluidic platform. This follows since the sensor must be compatible with the hydrophobic surfaces on which electrowetting liquid transport occurs. With the emergence of heterogeneous photonic component integration technologies such as those described herein, the opportunity for integrating advanced photonic components has expanded considerably. Many diagnostic applications could benefit from the integration of more advanced miniaturized optical sensing technologies, such as index of refraction sensors (surface plasmon resonance sensors, microresonator sensors, etc.). The advent of these heterogeneous integration technologies, that enable the integration of thin-film semiconductor devices onto arbitrary host substrates, enables more complex optical functions, and in particular, planar optical systems, to be integrated into microfluidic systems. This paper presents an integrated optical sensor based upon the heterogeneous integration of an InGaAs-based thin-film photodetector with a digital microfluidic system. This demonstration of the heterogeneous integration and operation of an active optical thin-film device with a digital microfluidic system is the first step toward the heterogeneous integration of entire planar optical sensing systems on this platform.

Chip Scale Optical Microresonator Sensors Integrated With Embedded Thin Film Photodetectors on Electrowetting Digital Microfluidics Platforms

Abstract: Miniaturized, portable, sensitive, and low cost sensing systems are important for medical and environmental diagnostic and monitoring applications. Chip scale integrated photonic sensing systems that combine optical, electrical, and fluidic functions are especially attractive for sensing applications due to the high sensitivity of optical sensors, the small form-factor of chip scale systems, and the low-cost processing possible for systems fabricated with well-developed mass production techniques. In this paper, a chip scale sensing system, which is composed of a planar integrated optical microdisk resonator and a thin film InGaAs photodetector, is integrated with a digital microfluidic system. This system was designed, fabricated, and experimentally characterized by dispensing and moving droplets of glucose solution from the reservoir to the microresonator sensor. The optical output of the resonator was transduced by the integrated photodetector to an electrical current signal for readout.

Optical Detection Heterogeneously Integrated With a Coplanar Digital Microfluidic Lab-on-a-Chip Platform

Abstract: The intimate integration of optical components with microfluidics technology will enable next generation portable LoC systems that may completely contain optical generation, processing, and detection. Toward the chip scale integration of digital microfluidics with active compound semiconductor devices, heterogeneous integration technology is used in this paper to integrate thin film (approx 1 micron thick) compound semiconductor photodetectors with digital microfluidic systems. Each thin film InGaAs photodetector is bonded onto a glass platform, coated with Teflon AF, and integrated into the digital microfluidics system. The detection function is tested using the mixing and digital droplet movement of chemiluminescent compounds, which clearly indicate the functionality of the microfluidics system and the integrated thin film InGaAs photodetector.

Digital microfluidics: is a true lab-on-a-chip possible?

Abstract: The suitability of electrowetting-on-dielectric (EWD) microfluidics for true lab-on-a-chip applications is discussed. The wide diversity in biomedical applications can be parsed into manageable components and assembled into architecture that requires the advantages of being programmable, reconfigurable, and reusable. This capability opens the possibility of handling all of the protocols that a given laboratory application or a class of applications would require. And, it provides a path toward realizing the true lab-on-a-chip. However, this capability can only be realized with a complete set of elemental fluidic components that support all of the required fluidic operations. Architectural choices are described along with the realization of various biomedical fluidic functions implemented in on-chip electrowetting operations. The current status of this EWD toolkit is discussed. However, the question remains: which applications can be performed on a digital microfluidic platform? And, are there other advantages offered by electrowetting technology, such as the programming of different fluidic functions on a common platform (reconfigurability)? To understand the opportunities and limitations of EWD microfluidics, this paper looks at the development of lab-on-chip applications in a hierarchical approach. Diverse applications in biotechnology, for example, will serve as the basis for the requirements for electrowetting devices. These applications drive a set of biomedical fluidic functions required to perform an application, such as cell lysing, molecular separation, or analysis. In turn, each fluidic function encompasses a set of elemental operations, such as transport, mixing, or dispensing. These elemental operations are performed on an elemental set of components, such as electrode arrays, separation columns, or reservoirs. Examples of the incorporation of these principles in complex biomedical applications are described.

Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices.

Abstract: Optical microcavities that confi ne light in high-Q resonance promise all of the capabilities required for a successful nextgeneration microsystem biodetection technology. Label-free detection down to single molecules as well as operation in aqueous environments can be integrated cost-effectively on microchips, together with other photonic components, as well as electronic ones. We provide a comprehensive review of the sensing mechanisms utilized in this emerging fi eld, their physics, engineering and material science aspects, and their application to nanoparticle analysis and biomolecular detection. We survey the most recent developments such as the use of mode splitting for self-referenced measurements, plasmonic nanoantennas for signal enhancements, the use of optical force for nanoparticle manipulation as well as the design of active devices for ultra-sensitive detection. Furthermore, we provide an outlook on the exciting capabilities of functionalized high-Q microcavities in the life sciences.

Enzyme-based logic systems for information processing

Abstract: In this critical review we review enzymatic systems which involve biocatalytic reactions utilized for information processing (biocomputing). Extensive ongoing research in biocomputing, mimicking Boolean logic gates has been motivated by potential applications in biotechnology and medicine. Furthermore, novel sensor concepts have been contemplated with multiple inputs processed biochemically before the final output is coupled to transducing “smart-material” electrodes and other systems. These applications have warranted recent emphasis on networking of biocomputing gates. First few-gate networks have been experimentally realized, including coupling, for instance, to signal-responsive electrodes for signal readout. In order to achieve scalable, stable network design and functioning, considerations of noise propagation and control have been initiated as a new research direction. Optimization of single enzyme-based gates for avoiding analog noise amplification has been explored, as were certain network-optimization concepts. We review and exemplify these developments, as well as offer an outlook for possible future research foci. The latter include design and uses of non-Boolean network elements, e.g., filters, as well as other developments motivated by potential novel sensor and biotechnology applications (136 references).

Droplet Actuation by Electrowetting-on-Dielectric (EWOD): A Review

Abstract: This paper reviews publications that have fortified our understanding of the electrowetting-on-dielectric (EWOD) actuation mechanism. Over the last decade, growing interest in EWOD has led to a wide range of scientific and technological investigations motivated by its applicability in microfluidics, especially for droplet-based optical and lab-on-a-chip systems. At this point in time, we believe that it is helpful to summarize the observations, insights, and modeling techniques that have led to the current picture showing how forces act on liquid droplets and how droplets respond in EWOD microfluidic devices. We discuss the basic physics of EWOD and explain the mechanical response of a droplet using free-body diagrams. It is our hope that this review will inspire new research approaches and help design useful devices.