R. E. Purnell

Bio: R. E. Purnell is an academic researcher from United Nations Development Programme. The author has contributed to research in topics: Theileria parva. The author has an hindex of 2, co-authored 2 publications receiving 246 citations.

Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva

Abstract: Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva

The incidence of theilerial parasites in East African buffalo (Syncerus caffer).

Abstract: 245 buffalo from 13 areas of East Africa were examined for theilerial infections. The vast majority of buffalo (97.1%) examined had piroplasms in their erythrocytes. Theileria lawrencei was isolated from the buffalo by tick feeding and cell culture and was found to be common in most of these buffalo populations. Also over 50% of the buffalo had indirect fluorescent antibody (IFA) titres to T. lawrencei. T. mutans was only isolated from 3 buffalo populations but is probably common. Haematoxenus sp. was detected in the blood of 56% of the buffalo sampled. In the light of these results the role of buffalo as a reservoir of cattle pathogenic theilerioses in East Africa is discussed.

Games parasites play: how parasites evade immune surveillance

Abstract: Parasites have evolved an extraordinary variety of mechanisms for surviving in the face of the natural and acquired immune responses of their hosts. A selection of the mechanisms serves to illustrate the challenge involved in developing strategies for immunising against parasites.

Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences.

Abstract: The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutants and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever.

Transformation of Leukocytes by Theileria parva and T. annulata

Abstract: Theileria parva and T. annulata provide intriguing models for the study of parasite-host interactions. Both parasites possess the unique property of being able to transform the cells they infect; T. parva transforms T and B cells, whereas T. annulata affects B cells and monocytes/macrophages. Parasitized cells do not require antigenic stimulation or exogenous growth factors and acquire the ability to proliferate continuously. In vivo, parasitized cells undergo clonal expansion and infiltrate both lymphoid and non-lymphoid tissues of the infected host. Theileria-induced transformation is entirely reversible and is accompanied by the expression of a wide range of different lymphokines and cytokines, some of which may contribute to proliferation or may enhance spread and survival of the parasitized cell in the host. The presence of the parasite in the host-cell cytoplasm modulates the state of activation of a number of signal transduction pathways. This, in turn, leads to the activation of transcription factors, including nuclear factor-kappa B, which appear to be essential for the survival of Theileria-transformed T cells.

Integrated control of ticks and tick-borne diseases of cattle in Africa.

Abstract: The problems caused by tick and tick-borne diseases for livestock particularly cattle on the African continent are described and discussed. The control of ticks and tick-borne diseases must receive high priority in Africa with regard to both research and control application because of their widespread distribution in areas of high livestock potential and productivity. The conventional methods of tick and tick-borne disease control are discussed and are found to be inadequate in the conditions prevailing in Africa. Methods of integrated control are suggested and discussed in light of recent development in control methods and those still under development. Any one of these methods may not be adequate to control the problem on its own but when several of the methods are combined an economic and robust integrated control is likely to result. Encouragement is given to attempt this approach in Africa to solve what must be the largest animal health problem of livestock remaining in the world.

Adoptive transfer of immunity to Theileria parva in the CD8+ fraction of responding efferent lymph.

Abstract: Evidence that class I major histocompatibility complex-restricted cytotoxic T lymphocytes (CTL) are involved in immunity to malaria has highlighted the potential importance of these cells in protection against intracellular parasites. Parasite-specific CTL are a prominent feature of the immune response of cattle to Theileria parva, a related apicomplexan parasite. The relationship between the appearance of these cells in the blood of immune cattle under challenge and the clearance of infection suggests that they are involved in the control of infection, but direct evidence is lacking that CTL can mediate protection. We have made a quantitative kinetic study of CTL responses in lymph originating from infected lymph nodes in a number of immune cattle under challenge with T. parva. Direct killing activity and the frequency of CTL precursors (CTLp) within responding cell populations were evaluated. A substantial increase in the proportion of CD8+ CTL was observed between days 8 and 11 after challenge. Frequencies of CTLp as high as 1:32 were observed and activity was essentially confined to the large blasting cell fraction. The analogous response in peripheral blood was of lower magnitude and delayed by 1-2 days. The high frequency of CTLp in efferent lymph permitted the adoptive transfer of this activity between immune and naive monozygotic twin calves. In separate experiments, naive calves lethally infected with T. parva were protected by inoculation of up to 10(10) responding CD8+ T cells derived from their immune twins. Elimination of CD8+ T cells within the inoculum abrogated this effect. These findings provide direct evidence that CD8+ T cells can control T. parva infections in immune cattle.