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R. P. C. Gerger
Researcher at University of São Paulo
Publications - 16
Citations - 109
R. P. C. Gerger is an academic researcher from University of São Paulo. The author has contributed to research in topics: Blastocyst & Somatic cell. The author has an hindex of 5, co-authored 16 publications receiving 100 citations. Previous affiliations of R. P. C. Gerger include Universidade do Estado de Santa Catarina.
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Journal ArticleDOI
Developmental Potential of Bovine Hand-Made Clone Embryos Reconstructed by Aggregation or Fusion with Distinct Cytoplasmic Volumes
Eduardo S. Ribeiro,R. P. C. Gerger,Lain Uriel Ohlweiler,Ivens Ortigari,Joana Claudia Mezzalira,Fabiana Forell,Luciana Relly Bertolini,José Luiz Rodrigues,Carlos Eduardo Ambrósio,Maria Angélica Miglino,Alceu Mezzalira,Marcelo Bertolini +11 more
TL;DR: The increase in cytoplasmic volume, either by fusion or by aggregation, had a positive effect on embryo development, supporting the establishment of pregnancies and the birth of a viable clone calf after transfer to recipients.
Journal ArticleDOI
In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence.
R. P. C. Gerger,Eduardo S. Ribeiro,Fabiana Forell,Luciana Relly Bertolini,José Luiz Rodrigues,Carlos Eduardo Ambrósio,M. A. Miglino,Alceu Mezzalira,Marcelo Bertolini +8 more
TL;DR: It is concluded that degree of cell culture confluence significantly influences subsequent embryo development; use of a cell population in high confluence for nuclear transfer significantly improved blastocyst yield after cloning.
Journal ArticleDOI
Morphometric developmental pattern of bovine handmade cloned concepti in late pregnancy.
R. P. C. Gerger,F. C. Zago,Eduardo S. Ribeiro,S. Gaudencio Neto,L. T. Martins,L. H. Aguiar,V. H. V. Rodrigues,F H Furlan,Ivens Ortigari,Roberto Daniel Sainz,Calvin L. Ferrell,M. A. Miglino,Carlos Eduardo Ambrósio,José Luiz Rodrigues,Rafael Rossetto,Fabiana Forell,Luciana Relly Bertolini,Marcelo Bertolini +17 more
TL;DR: Morphological changes in cloned concepti likely affected placental function and metabolism, disrupting the placental constraining mechanism on fetal growth in mid- to late pregnancy.
Journal ArticleDOI
Production of bovine hand-made cloned embryos by zygote-oocyte cytoplasmic hemi-complementation.
Joana Claudia Mezzalira,Lain Uriel Ohlweiler,R. P. C. Gerger,R. P. C. Gerger,R. Casali,F. K. Vieira,Carlos Eduardo Ambrósio,Maria Angélica Miglino,José Luiz Rodrigues,Alceu Mezzalira,Marcelo Bertolini +10 more
TL;DR: The presence of cytoplasmic factors from MII hemi-oocytes and the sperm activation process from hemi’szygotes appear to be necessary for adequate in vitro development, as only the zygote-oocyteHemi-complementation was as efficient as controls for the generation of bovine cloned blastocysts.
Journal ArticleDOI
Impact of cumulative gain in expertise on the efficiency of handmade cloning in cattle
R. P. C. Gerger,R. P. C. Gerger,Rafael Rossetto,Eduardo S. Ribeiro,Ivens Ortigari,Fabiano Carminatti Zago,L. H. Aguiar,Ubirajara Maciel da Costa,Rui Fernando Felix Lopes,Carlos Eduardo Ambrósio,Maria Angélica Miglino,José Luiz Rodrigues,Fabiana Forell,Luciana Relly Bertolini,Marcelo Bertolini +14 more
TL;DR: Results demonstrated a gradual improvement in in vitro and in-vivo embryo development over time after establishment of HMC procedures in the laboratory, highlighting the importance of gaining experience and technical skills on the overall cloning efficiency.