scispace - formally typeset
Search or ask a question
Author

R. Suja

Bio: R. Suja is an academic researcher. The author has contributed to research in topics: Silver nanoparticle & Hydrogen bond. The author has an hindex of 2, co-authored 2 publications receiving 46 citations.

Papers
More filters
01 Jan 2013
TL;DR: In this paper, the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens and its cytotoxic activity against MCF-7 breast cancer cell line.
Abstract: Objective: Vegetable mediated synthesis of nanoparticles is a green chemistry approach that links Nanotechnology and Biotechnology. The present study is focussed on the biosynthesis of silver nanoparticles using aqueous fresh Cauliflower floret extract and to investigate the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens and its cytotoxic activity against MCF-7 breast cancer cell line. Methods: It was found that aqueous silver ions when treated with aqueous extract of fresh Cauliflower floret are reduced in solution there by leading to the formation of silver nanoparticles under optimum conditions at pH 6. The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles was characterised using several techniques, viz- UV-Vis spectroscopy, FT-IR, XRD, TEM, SEM and EDAX analysis. The free radical scavenging potential was measured by DPPH assay, antimicrobial activity against four microorganisms was tested using disc diffusion method and cytotoxcity of the nanoparticles was determined against MCF-7 cell line at different concentrations by MTT assay. Results: Water soluble antioxidant constituents present in the Cauliflower floret extract were mainly responsible for the reduction of silver ions to nanosized Ag particles. UV-Vis spectral analysis showed silver Surface Plasmon Resonance band at 425nm. The presence of active proteins and phenolic groups present in the biomass before and after reduction was identified by FT-IR. The presence of elemental silver was characterised by EDS. The crystalline morphology and size of the nanoparticles were determined by TEM, SEM, and X-ray diffraction studies which showed the average size of the nanoparticles in the range 40 –50nm, as well as revealed their FCC structure. The biologically synthesized nanoparticles efficiently inhibited pathogenic organisms such as Klebsiella Pneumonia, Staphylococcus Saprophyticus and E. Coli. The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH assay. Further these nanoparticles efficiently showed reduced viability and increased cytotoxicity on MCF-7 breast cancer cell line in a dose dependent manner. Conclusion: The present investigation revealed that the fresh aqueous Cauliflower floret extract are capable of producing silver nanoparticles extra cellularly through green synthesis and the reduction process was proved to be good, competent, convenient, easy to handle and considered ecofriendly as an alternative route to physical and chemical methods. The biosynthesized nanoparticles are quite stable in aqueous solution for a month without any sign of precipitation.

24 citations

Journal Article
TL;DR: In this paper, a green chemistry approach for the biological synthesis of silver nanoparticles using Broccoli floret aqueous extract under optimum conditions was reported, where the reduction process was simple and convenient to handle and monitored by UV-Vis spectroscopy which showed surface plasmon resonance at 425nm and the presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FT-IR.
Abstract: OBJECTIVE– The synthesis of metal nanoparticles is a growing area of research due to its potentiality in the application and development of advanced technologies In general, nanoparticles are synthesized by using chemical methods which are not eco-friendly Vegetable mediated synthesis of nanoparticles is a green chemistry approach that connects the nanotechnology and biotechnology In the present investigation we report a green chemistry approach, for the biological synthesis of silver nanoparticles using Broccoli floret aqueous extract under optimum conditions METHOD- Here we have used a fast, convenient and environment friendly method for the synthesis of silver nanoparticles by biologically reducing AgNO₃ with aqueous extract of Broccoli florets (Brassica Oleracea L var Italica) under optimum conditions (pH-6-7) The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown Biosynthesized nanoparticles were characterized by UV-VIS, FT-IR, XRD, SEM, TEM and EDAX analysis The free radical scavenging activity was assessed by DPPH assay These biologically synthesised Ag nanoparticles were tested for antimicrobial activity against four human pathogens viz Klebsiella Pneumonia, Staphylococcus Saprophyticus, Bacillus Cereus and Escheria Coli These nanoparticles were assessed further for cytotoxic activity on MCF-7 cell line RESULT- The reduction process was simple and convenient to handle and monitored by UV-Vis spectroscopy which showed surface plasmon resonance at 425nm The presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FT-IR The crystalline morphology and size of the nanoparticles were determined by TEM, SEM AND X-ray diffraction studies, which showed the average particle size of silver nanoparticles was in the range 40- 50nm as well as revealed their FCC structure Presence of elemental silver was revealed by EDAX analysis These biologically synthesised Ag nanoparticles were found to be effective in controlling growth of human pathogens viz Klebsiella Pneumonia, Staphylococcus Saprophyticus and Escheria Coli These nanoparticles showed high percent toxicity against MCF-7 cell line The reducing property of aqueous extract is due to the presence of antioxidant viz ascorbic acid, polyphenols which is confirmed by quantitative assay and scavenging effect of free radicals proved by DPPH scavenging activity CONCLUSION- The present investigation revealed that the fresh Broccoli floret aqueous extract is capable of producing silver nanoparticles that are quiet stable for 15 days at room temperature without any sign of precipitation Keywords: Brassica oleracea Lvaritalica, SEM, TEM, XRD, EDAX, FT-IR, Scherrer formula and Cytotoxic effect

22 citations

Journal ArticleDOI
TL;DR: Piperazine succinate (PZSN) has been synthesized and extensively analyzed using various spectroscopic techniques, including FT-IR, FT-Raman and NMR spectra as mentioned in this paper .

Cited by
More filters
Journal ArticleDOI
TL;DR: Synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast and human colon cancer cell lines.
Abstract: Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.

407 citations

Journal ArticleDOI
TL;DR: In vivo studies clearly indicated that AgNPs has a capacity to inhibit the growth of tumor in Ehrlich ascites carcinoma bearing mice and face-centered central composite design used for optimization of silver nanoparticles (AgNPs) biosynthesis using phycocyanin.
Abstract: In recent decades, researchers were attracted towards cyanobacterial components which are potential low-cost biological reagents for silver nanoparticle biosynthesis. This article describes the biological synthesis of silver nanoparticles using a proteinaceous pigment phycocyanin extracted from Nostoc linckia as reducing agent. The synthesized silver nanoparticles have a surface plasmon resonance band centered at 425 nm. Face-centered central composite design used for optimization of silver nanoparticles (AgNPs) biosynthesis using phycocyanin. The maximum AgNPs biosynthesis obtained using the optimized four variables, initial pH level (10), AgNO3 concentration (5 mM), phycocyanin pigment concentration (1 mg/mL) and incubation period (24 h) was 1100.025 µg/mL. The TEM analysis of AgNPs showed spherical nanoparticles with mean size between 9.39 to 25.89 nm. FTIR spectra showed major peaks of proteins involved in AgNPs biosynthesis by identifying different functional groups involved in effective capping of AgNPs. The biosynthesized AgNPs significantly inhibited the growth of medically important pathogenic Gram-positive (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa, E. coli and Klebsiella pneumonia). The synthesized AgNPs exhibited effective cytotoxic activity against MCF-7 and the inhibitory concentration (IC50) was recorded at 27.79 ± 2.3 µg/mL. The in vivo studies clearly indicated that AgNPs has a capacity to inhibit the growth of tumor in Ehrlich ascites carcinoma bearing mice.

170 citations

Journal ArticleDOI
TL;DR: In this paper, Nanomedicine plays an important role in developing alternative and more effective treatment strategies for cancer, which is one of the most common health problems responsible for outnumbered deaths worldwide.
Abstract: Cancer is one of the most common health problems responsible for outnumbered deaths worldwide. Nanomedicine plays an important role in developing alternative and more effective treatment strategies...

155 citations

Journal ArticleDOI
TL;DR: This article highlights the green approach of nanomaterials synthesis using microbes and current status of their applications in biomedical field.

123 citations

Journal ArticleDOI
TL;DR: The synthesis of silver nanoparticles using the aqueous extract of Alternanthera sessilis as a reducing agent by sonication is described, espousing green chemistry principles, to reveal the potent therapeutic value of biogenicSilver nanoparticles and the scope for further development of anticancer drugs.
Abstract: This article describes the synthesis of silver nanoparticles using the aqueous extract of Alternanthera sessilis as a reducing agent by sonication, espousing green chemistry principles. Biologically synthesized nanoparticle-based drug delivery systems have significant potential in the field of biopharmaceutics due to its smaller size entailing high surface area and synergistic effects of embedded biomolecules. In the present work the cytotoxic effect of biosynthesized silver nanoparticles studied by MTT assay against breast cancer cells (MCF-7 cell line) showed significant cytotoxic activity with IC50 value 3.04 μg/mL compared to that of standard cisplatin. The superior activity of the silver nanoparticles may be due to the spherical shape and smaller particle size 10–30 nm as confirmed from transmission electron microscope (TEM) analysis. The data obtained in the study reveal the potent therapeutic value of biogenic silver nanoparticles and the scope for further development of anticancer drugs.

97 citations