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Rahman S. Mizanur

Bio: Rahman S. Mizanur is an academic researcher. The author has contributed to research in topics: Agrobacterium tumefaciens & Phosphinothricin acetyltransferase. The author has an hindex of 1, co-authored 1 publications receiving 10 citations.

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Journal ArticleDOI
TL;DR: The presence of transgenes in transformed azuki bean plants was confirmed by polymerase chain reaction (PCR) and southern blot analysis and demonstrates the feasibility of introducing potentially useful agronomic traits into azukibean through genetic engineering.
Abstract: Transgenic azuki bean [ Vigna angularis (Willd.) Ohwi & Ohashi] plants expressing the hygromycin phosphotransferase ( hpt ), green fluorescent protein ( sgfp ) and phosphinothricin acetyltransferase ( bar ) genes were obtained by Agrobacterium- tumefacients - mediated transformation. A total of 210 epicotyl explants were inoculated with A. tumefaciens strain EHA105, harboring the binary plasmid pZHBG on MS co-cultivation medium supplemented with 100 mM acetosyringone and 10 mg/l of BA. Following selection on MS medium with 15 mg/l of hygromycin, the regenerated adventitious shoots that formed on the induced calli were further screened for sgfp expression before transferred to rooting medium. 31 transgenic plants were obtained with transformation frequency of 14%. The presence of transgenes in transformed azuki bean plants was confirmed by polymerase chain reaction (PCR) and southern blot analysis. Transcription of the bar and hpt genes was assessed by reverse transcription polymerase chain reaction (RT-PCR) analysis. sgfp- positive transgenic plants exhibited functional expression of the bar gene as determined by assaying for resistance to bialaphos applied directly to leaves. This result demonstrates the feasibility of introducing potentially useful agronomic traits into azuki bean through genetic engineering. Key Words: Agrobacterium tumefaciens, bar gene, bialaphos, transgenic, Vigna angulazris . African Journal of Biotechnology Vol.4(1) 2005: 61-67

10 citations


Cited by
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Journal ArticleDOI
TL;DR: With optimization of various factors which influence genetic transformation of pulse crops, it will be possible to develop transgenic plants in this important group of crop species with more precision and reproducibility, according to the reason for lack of commercialization of transgenic pulse crops.

68 citations

Journal ArticleDOI
TL;DR: Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and α-amylase inhibitor, have been developed for the first time and inheritance of both thetransgenes in most of the T1 lines was revealed.
Abstract: Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and α-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos resistance (bar) gene and Phaseolus vulgaris α-amylase inhibitor-1 (αAI-1) gene. Green transformed shoots were regenerated and rooted on medium containing phosphinothricin (PPT). Preculture and wounding of the explants, presence of acetosyringone and PPT-based selection of transformants played significant role in enhancing transformation frequency. Presence and expression of the bar gene in primary transformants was evidenced by PCR-Southern analysis and PPT leaf paint assay, respectively. Integration of the Phaseolus vulgaris α-amylase inhibitor gene was confirmed by Southern blot analysis. PCR analysis revealed inheritance of both the transgenes in most of the T1 lines. Tolerance to herbicide was evidenced from seed germination test and chlorophenol red assay in T1 plants. Transgenic plants could be recovered after 8–10 weeks of cocultivation with Agrobacterium. An overall transformation frequency of 1.51% was achieved.

65 citations

Journal ArticleDOI
TL;DR: A step-wise procedure for the regeneration of fertile plants by organogenesis from cultures of the economically important Phaseolus angularis L., cultivars: KS-6, KS-7 and KS-8 using etiolated seedlings was established, finding the efficient shoot bud induction capability to be cultivar dependent.
Abstract: A step-wise procedure for the regeneration of fertile plants by organogenesis from cultures of the economically important Phaseolus angularis L., cultivars: KS-6, KS-7 and KS-8 using etiolated seedlings was established. Pre-culture of 5-day old seedling explants with MS (Murashige and Skoog (1962) Physiol Plant 15:473–493) + B5-vitamins (Gamborg et al. (1968) Exp Cell Res 50:151–158) liquid medium containing either 5.0 µM TDZ or 5.0 µM BAP under dark condition was essential for organogenesis. Bud growth and shoot multiplication were stimulated by reducing the BAP concentrations from 5.0 to 2.5 µM after 3 weeks. The maximum frequency of shoot induction was 65.2% (33.8 ± 2.54 shoots/explant) in cultivar KS-8 followed by KS-7 34.6% (23.4 ± 1.91 shoots/explant) and KS-6 30.6% (21.2 ± 2.28 shoots/explant). The multiplied buds elongated after transferring to solid MSB5 medium supplemented with 4.0 µM GA3, 12.5 µM AgNO3 and 0.4 µM IBA. Up to 98% rooting efficiency of was obtained when the shoots were pulse-treated with liquid medium containing 4.5 µM IBA for 10 min. The rooted plantlets were transferred to pots in the greenhouse, where they grew, mature, flowered and bared pod normally. The efficient shoot bud induction capability was found to be cultivar dependent. All the three cultivars tested formed multiple shoots. This efficient and rapid regeneration system may also be helpful for Agrobacterium- or particle gun-mediated transformation for this important legume crop.

45 citations

Journal ArticleDOI
TL;DR: This article examines the prospects of genomics assisted integrated breeding to enhance and stabilize crop yields and outlines the recent progress made in genomics of these lesser explored pulse crops.

33 citations

Book ChapterDOI
01 Jan 2019
TL;DR: DNA marker analysis suggests that there are obvious genetic distinctions between different forms, but the diversity among cultivated germplasm is quite low, indicating that the wild forms could be an important genetic resource for breeding.
Abstract: Adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi], an annual pulse crop, belongs to the genus Vigna and subgenus Ceratotrapis. It provides nutritional elements for the human diet and fertilizes soil by nitrogen fixation. It has been traditionally planted and consumed in East and Southeast Asia, especially in China, Japan and Korea, so it came to be called the Asia legume. Adzuki bean was dispersed to other continents for commercial uses in recent decades. Wild adzuki bean (V. angularis var. nipponensis), considered to be the ancestor of cultivated adzuki bean, occurs in East Asia and in the Himalayan Region, which are presumed to be where the domestication of adzuki bean took place. Another wild form, V. nepalensis, called the weedy adzuki bean, is mainly found in Eastern Nepal and around. A large portion of adzuki bean germplasm has been collected and conserved in different gene banks. DNA marker analysis suggests that there are obvious genetic distinctions between different forms, but the diversity among cultivated germplasm is quite low, indicating that the wild forms could be an important genetic resource for breeding. However, the genetic and genomic studies on this species are lagging and include only low-density genetic maps and a few maps of genes. That is the reason conventional breeding of adzuki bean has achieved rapid improvement, while no modern biotechnology has yet been used in breeding.

7 citations