Showing papers by "Rama Shanker Verma published in 2008"
TL;DR: An improved method for rapid isolation of cardiomyocytes from neonatal mice, as well as the maintenance and propagation of such cultures for the long term is described.
Abstract: The primary culture of neonatal mice cardiomyocyte model enables researchers to study and understand the morphological, biochemical, and electrophysiological characteristics of the heart, besides being a valuable tool for pharmacological and toxicological studies. Because cardiomyocytes do not proliferate after birth, primary myocardial culture is recalcitrant. The present study describes an improved method for rapid isolation of cardiomyocytes from neonatal mice, as well as the maintenance and propagation of such cultures for the long term. Immunocytochemical and gene expression data also confirmed the presence of several cardiac markers in the beating cells during the long-term culture condition used in this protocol. The whole culture process can be effectively shortened by reducing the enzyme digestion period and the cardiomyocyte enrichment step.
105 citations
TL;DR: Continuous efforts are being made to investigate molecules exclusively expressed on cancer cells, to improve the specificity and efficacy of these immunotoxins, and to eliminate side effects and improve pharmacokinetics and ensure better drug delivery.
58 citations
TL;DR: In contrast to above results with MRSA and MSSA, mecA detection by PCR in MS-CoNS showed less correlation with disk susceptibility tests, and the results for coag Detection by PCR were consistent with phenotypic tests in all isolates.
34 citations
TL;DR: It is demonstrated that ERIC-PCR is a simple, rapid, and inexpensive tool with sufficient discriminatory power and is applicable for characterization and genotyping of a large number of clinical isolates of S. agalactiae at molecular level.
Abstract: Streptococcus agalactiae is reported to be an asymptomatic vaginal colonizer in Indian women, although it is considered one of the major causes of neonatal infections in many European countries. DNA based molecular typing methods are more reliable than the conventional serotyping method for identification and typing of this pathogen. In the present study, we have evaluated genetic diversity among colonizing S. agalactiae strains (n=86) by using a PCR-based genotyping method i.e. Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR). With ERIC-PCR fingerprinting at 60% similarity level in a dendrogram generated by UPGMA cluster analysis, 10 different ERIC groups were identified, which were subdivided into 62 distinct genotypes at ≥ 95% similarity level. Based on these findings, we demonstrate that ERIC-PCR is a simple, rapid, and inexpensive tool with sufficient discriminatory power and is applicable for characterization and genotyping of a large number of clinical isolates of S. agalactiae at molecular level.
7 citations
TL;DR: Gene patterns and sequences of folic acid synthesizing genes that are converged as meaningful patterns during evolution in the higher eukaryotes has been identified using sequence alignment and pattern analysis.
Abstract: Gene patterns and sequences of folic acid synthesizing genes that are converged as meaningful patterns during evolution in the higher eukaryotes has been identified using sequence alignment and pattern analysis. Based on the finding, we are postulating that part of genes that are involved in synthesis of folic acid in lower eukaryotes, are converged into meaningful similar functional domains of folate receptor in higher eukaryotes.
2 citations