Rani Asmarayani

Bio: Rani Asmarayani is an academic researcher from Gadjah Mada University. The author has contributed to research in topics: Piper & Ceratocystis. The author has an hindex of 1, co-authored 1 publications receiving 10 citations.

Relationship of species Piper based on morphological and leaf essential oils characters in Yogyakarta

Abstract: Some of Piper species were used for traditional medicines and condiments The leaf essential oil (terpenoid) of those Piper species usually were used as a main component in traditional medicine The taxonomycal study was aimed to determined Piper species relationships based on morphological and leaf essential oils characters The plants were obtained by exploring this province, and samples were collected for identification and leaf essential oils isolation purposes Species identification were carried out based on identification key (Backer and Bakhuizen vd Brink, 1965; Heyne, 1987; Shaorong, 1982) The isolation of leaf essential oils was carried out using Stahl destillation method, and their composition were interpreted with liquid gas chromatography, using caryophyllene and ii-pinene as a standard of essential oils component Dendrogram, which showed phenetic relationships among those species, were obtained by hierarchical cluster analysis method Results of the research showed that there were 8 species found as cultivated plants in Yogyakarta, which were P miniatum Bl, P betle L, P recurvum Bl, P aduncum L, P nigrum L, P cubeba Lf, P retrofractum Vahl, and P sarmentosum Roxb Ex Hunter Relationship between species of Piper based on morphological character showed that P aduncum and P sarmentosum at thesame cluster on 692% similarity level, and 404% similarity level to the other clusters Relationsips between species of Piper based on leafessential oils character resulted the difference cluster among the species, P retrofractum separated from the other species at 455% similarity level, P aduncum and P cubeba indicated the higest similarity level (815%)@ 2004 Jurusan Biologi FMIPA UNS SurakartaKey words: relationship, Piper, morphology, leaf essential oils, taxonomic characters

Antifungal activity of three Piper species against Ceratocystis fimbriata in rubber trees

Abstract: Abstract The research aimed to investigate the effect of leaf extracts of Piper cilibracteum, P. curtisii and P. aduncum againts of Ceratocystis fimbriata in vitro. Crude methanolic extract of P. aduncum leaves showed the highest percentage growth inhibition (GIP; 79.38%) than those of P. cilibracteum (37.52%) and P. curtisii (24.31%). Further examination of the leaf extract of P. aduncum with various solvents, we found that the n-hexane extract had the best performance in inhibiting the growth of C. fimbriata (GIP of 73.20%). The Minimum Inhibition Concentration (MIC)50 and MIC90 of this n-hexane extract towards C. fimbriata were reached at a very low concentration at 165 mg/L and 6999 mg/mL, respectively. The n-hexane leaf extract of P. aduncum is a potential candidate for anti-Ceratocystis agent. Based on Gas Chromatography-Mass Spectra of n-hexane leaves extract of P. aduncum, apiol was identified as a major compound in the extract. HIGHLIGHTS • Leaf extract of P. cilibracteum, P. curtisii and P. aduncum had antifungal against Ceratocystis fimbriata. • N-hexane extract of P. aduncum showed greater growth inhibition to C. fimbriata. • GC-MS spectra of n-hexane extract of P. aduncum showed apiol as a major compound. • Piper aduncum can be considered as an antifungal resource for protection of rubber crops.

Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

Abstract: The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium + 1.3M dimethyl sulphoxide + 0.3M glucose + 2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as the treatment (T) and fresh semen as the control (F). Straws of 0·5 ml of sperm suspension were frozen in 4 cm of N2 L. They were thawed in a thermoregulated bath (40° C). After thawing, the percentage of spermatozoa with fragmented DNA [transferase dUTP (deoxyuridine triphosphate) nick-end labelling (TUNEL)], plasma membrane integrity (SYBR-14/PI) and mitochondrial membrane potential (ΔΨMMit, JC-1) were evaluated by flow cytometry and motility was evaluated by optical microscope under stroboscopic light. The fertilization rates of the control and treatment semen were tested at a sperm density of 1·5 × 10(7) spermatozoa oocyte(-1) , by observation of the first cleavages after 16 h incubation at 10° C. In the cryopreserved semen (T), the mean ± s.d. DNA fragmentation was 4·8 ± 2·5%; plasma membrane integrity 75·2 ± 6·3%; mitochondrial membrane potential 51·7 ± 3·6%; motility 58·5 ± 5·3%; curved line velocity (VCL ) 61·2 ± 17·4 µm s(-1) ; average-path velocity (VAP ) 50·1 ± 17·3 µm s(-1) ; straight-line velocity (VSL ) 59·1 ± 18·4 µm s(-1) ; fertilization rate 81·6 ± 1·9%. There were significant differences in the plasma membrane integrity, mitochondrial membrane potential, motility, fertilization rate, VCL , VAP and VSL compared with the controls (P < 0·05). Also the mitochondrial membrane potential correlated with motility, fertilization rate, VCL and VSL (r = 0·75; r = 0·59; r = 0·77 and r = 0·79, respectively; P < 0·05); and the fertilization rate correlated with VCL and VSL (r = 0·59 and r = 0·55, respectively).

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Abstract: This study aimed to investigate the effects of different doses of taurine added to glucose extender. Semen was collected from rainbow trout (Oncorhynchus mykiss) by abdominal massage and diluted (1:2) with 300 mM glucose (G) extender containing different taurine doses (50, 75 and 100 mM). The control group extender did not contain taurine. After dilution and dosing, samples were filled to 0.5 ml straws, frozen in nitrogen vapour and stored in liquid nitrogen. The comparison of the results obtained for the 50 mM taurine group and the control group showed no significant difference between the post-thawing motility percentages and motility duration of sperm, and only a very low statistically difference for eyed-embryo percentages (P < 0.05).