Raphael Nyaruaba

TL;DR: A method to identify SARS-CoV-2 (COVID-19) virus RNA from purified RNA or cell lysis using loop-mediated isothermal amplification (LAMP) using a visual, colorimetric detection is reported.

TL;DR: From current studies, it is clear that ddPCR technology offers enormous advantages, such as unparalleled sensitivity, high precision, and absolute quantification without a standard curve, over common molecular diagnostic platforms like the real-time quantification PCR.

TL;DR: Wang et al. as mentioned in this paper demonstrated the significance of wastewater-based epidemiology in continuous surveillance of SARS-CoV-2 at the community level, even when the COVID-19 prevalence is low.

TL;DR: In this paper, the authors developed, optimized, and analyzed the performance of simplex (1 target), duplex (2 targets), triplex probe mix (3 targets), and quadruplex (4 targets) SARS-CoV-2 ddPCR assays based on a two-color DDPCR detection system.

TL;DR: Characteristics of 24 mosquito-borne arboviruses indigenous to the EAC are reviewed, including lesser or poorly understood viruses, like Batai virus (BATV) and Ndumu virus (NDUV), which may escape their origins under perfect conditions to establish a foothold in new geographical locations.