Renaud Léonard

TL;DR: In vivo protein sialylation in plants is reported, which has been shown to be well suited for the efficient generation of complex mammalian glycoproteins and may serve as a general model for the manipulation of complex traits into plants.

TL;DR: Results demonstrate that GALT1 is both sufficient and essential for the addition of β1,3-linked galactose residues to N-glycans and thus is required for the biosynthesis of Lewis a structures in Arabidopsis.

TL;DR: In this paper, a β-N-acetylglucosaminidase with a potential transmembrane domain was cloned and expressed in Pichia pastoris, which exhibited a substrate specificity similar to that previously described for a hexosamidase activity from Sf-9 cells, i.e., it hydrolyzed exclusively the GlcNAc residue attached to the α 1,3-linked mannose of the core pentasaccharide of N-glycans.

TL;DR: The fdl gene encodes a novel hexosaminidase responsible for the occurrence of paucimannosidic N-glycans in Drosophila, and the ratio of structures with terminal GlcNAc over those without was drastically increased in the fdl-deficient flies.

TL;DR: Although the authors could not observe antibody binding by the polysaccharide, the single hydroxyproline-linked β-arabinose residues appeared to react with the antibodies and constitute a new, potentially cross-reactive, carbohydrate determinant in plant proteins.