Renny Edwin Fernandez

Bio: Renny Edwin Fernandez is an academic researcher from Florida International University. The author has contributed to research in topics: Computer science & Sequence motif. The author has an hindex of 12, co-authored 31 publications receiving 447 citations. Previous affiliations of Renny Edwin Fernandez include Indian Institute of Technology Madras & Norfolk State University.

Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

Abstract: We report a disposable point-of-care sensing platform specific to salivary cortisol detection. The sensor is inkjet printed on a paper substrate with a metalloporphyrin based macrocyclic catalyst ink that can electrochemically reduce cortisol, captured by aptamer functionalized magnetic nanoparticles. The sensor consists of a thin magnet disc, aligned at the back of the electrode, in order to populate the magnetic nanoparticle bound cortisol at the sensing electrode area. Proof of concept studies were performed to detect salivary cortisol levels in human subjects with high and low risks for obstructive sleep apnea (OSA). High selectivity was observed to salivary cortisol against a background of closely related steroids.

Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper–plastic disposable microfluidic device using a smartphone

Abstract: We report the design and characterization of a disposable light shielded paper–plastic microfluidic device that can detect nanomolar levels of H2O2 using a smartphone camera and a light sealed accessory Chemiluminescence reaction of H2O2 with bis(2,4,6-trichlorophenyl)oxalate in the presence of rubrene and imidazole is confined to a paper reaction site where the individual reagents are delivered via plastic microfluidic channels The net photon emission from the chemiluminescence reactions, detected by using a smartphone, is correlated with H2O2 concentrations With a total reagent volume of 25 μL, the sensor system was able to detect H2O2 concentrations as low as 250 nM The smartphone based chemiluminescence sensing system has great potential as a point of care diagnostic tool for monitoring nanomolar levels of H2O2 in biological samples

Salivary cortisol analysis using metalloporphyrins and multi-walled carbon nanotubes nanocomposite functionalized electrodes

Abstract: Continuous monitoring of biomarkers in physiological fluids, such as blood, sweat or saliva, by means of low-cost and non-invasive devices, is of crucial importance to improve diagnosis and disease treatment. Herein, an electrochemical sensor fabricated using screen printed carbon electrodes (SPCE) have been investigated as a low-cost sensor platform for monitoring cortisol levels. The detection mechanism of the proposed sensor is based on electrochemical reduction of cortisol by metalloporphyrin (MTPP) at the multi-walled carbon nanotubes (MWCNTs) modified electrodes. Computational modelling studies and binding energy calculations have been performed to select a suitable MTPP for cortisol detection. The proposed screen printed cortisol sensor was used to accurately detect variations in salivary cortisol levels in young adult women. To validate the accuracy of the sensor, the results of salivary cortisol levels obtained with the sensors are compared with the commercial ELISA and found to be correlated.

Cryptosporidium Taxonomy: Recent Advances and Implications for Public Health (Review)

Abstract: There has been an explosion of descriptions of new species of Cryptosporidium during the last two decades This has been accompanied by confusion regarding the criteria for species designation, largely because of the lack of distinct morphologic differences and strict host specificity among Cryptosporidium spp A review of the biologic species concept, the International Code of Zoological Nomenclature (ICZN), and current practices for Cryptosporidium species designation calls for the establishment of guidelines for naming Cryptosporidium species All reports of new Cryptosporidium species should include at least four basic components: oocyst morphology, natural host specificity, genetic characterizations, and compliance with the ICZN Altogether, 13 Cryptosporidium spp are currently recognized: C muris, C andersoni, C parvum, C hominis, C wrairi, C felis; and C cannis in mammals; C baileyi, C meleagridis, and C galli in birds; C serpentis and C saurophilum in reptiles; and C molnari in fish With the establishment of a framework for naming Cryptosporidium species and the availability of new taxonomic tools, there should be less confusion associated with the taxonomy of the genus Cryptosporidium The clarification of Cryptosporidium taxonomy is also useful for understanding the biology of Cryptosporidium spp, assessing the public health significance of Cryptosporidium spp in animals and the environment, characterizing transmission dynamics, and tracking infection and contamination sources

Textbook of biochemistry with clinical correlations

Abstract: Textbook of biochemistry with clinical correlations , 4th edn TM Devlin, ed pp xvii + 1186, illustrated Wiley-Liss, New York, 1997 £2995, hardback This beautifully illustrated and well-written book, with an impressive array of authors, is aimed at both undergraduate and postgraduate level As the editor states in the preface, it is not intended to be a compendium of biochemistry but rather emphasises the biochemistry of mammalian cells The first 22 chapters cover …

Paper-Based Microfluidic Devices: Emerging Themes and Applications

Abstract: Applications Yuanyuan Yang,† Eka Noviana,† Michael P. Nguyen,† Brian J. Geiss,‡ David S. Dandy, and Charles S. Henry*,†,§ †Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States ‡Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, United States Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, United States ■ CONTENTS Fabrication 71 Hydrophobic/Solvent Barrier 72 Deposition 73 Flow and Injection Control 74 Three-Dimensional Devices 75 Incorporating Nonsensing Electrodes 75 Colorimetric Detection 75 Detectors and Readout 75 Reflectance-Based Measurement 75 Transmittance-Based Measurement 77 Instrument-Free Measurement 77 Biomedical Applications 77 Enzymatic Methods 77 Immunoassays 78 Other 79 Environmental Applications 79 Other Applications 80 Electrochemical Detection 80 Electrodes 80 Carbon Electrodes 81 Metallic Electrodes 81 Biological Applications 82 Glucose Sensors 82 Immunosensors 84 Other Examples 84 Environmental Applications 84 Other Technologies 85 Chemiluminescence and Electrochemiluminescence 85 Fluorescence 85 Surface-Enhanced Raman Spectroscopy 85 Separation 86 Preconcentration 86 Conclusions and Future Directions 87 Author Information 87 Corresponding Author 87 ORCID 87 Notes 87 Biographies 87 Acknowledgments 88 References 88