Author
Ricardo Garcia
Other affiliations: University of Oregon, Autonomous University of Madrid
Bio: Ricardo Garcia is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Microscopy & Nanolithography. The author has an hindex of 59, co-authored 256 publications receiving 15520 citations. Previous affiliations of Ricardo Garcia include University of Oregon & Autonomous University of Madrid.
Papers published on a yearly basis
Papers
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TL;DR: In this paper, the authors review the fundamentals, applications and future tendencies of dynamic atomic force microscopy (AFM) methods and present a detailed quantitative comparison between theoretical simulations and experiment.
1,908 citations
TL;DR: The basic principles, advantages and limitations of the most common AFM bioimaging modes are reviewed, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging.
Abstract: Atomic force microscopy (AFM) is a powerful, multifunctional imaging platform that allows biological samples, from single molecules to living cells, to be visualized and manipulated. Soon after the instrument was invented, it was recognized that in order to maximize the opportunities of AFM imaging in biology, various technological developments would be required to address certain limitations of the method. This has led to the creation of a range of new imaging modes, which continue to push the capabilities of the technique today. Here, we review the basic principles, advantages and limitations of the most common AFM bioimaging modes, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging. For each of these modes, we discuss recent experiments that highlight their unique capabilities.
649 citations
TL;DR: It is shown that caveolin-1 (Cav1) favors cell elongation in three-dimensional cultures and promotes Rho- and force-dependent contraction, matrix alignment, and microenvironment stiffening through regulation of p190RhoGAP.
628 citations
TL;DR: In this article, the authors investigated the effect of tip sample separation, free amplitude, and sample properties on the performance of a force microscope with an amplitude modulation feedback, and showed that in the attractive regime, a net attractive force dominates the amplitude reduction while in the repulsive regime, amplitude reduction is dominated by a net repulsive force.
Abstract: Attractive and repulsive tip-sample interaction regimes of a force microscope operated with an amplitude modulation feedback were investigated as a function of tip-sample separation, free amplitude, and sample properties. In the attractive regime, a net attractive force dominates the amplitude reduction while in the repulsive regime the amplitude reduction is dominated by a net repulsive force. The transition between both regimes may be smooth or steplike, depending on free amplitude and sample properties. A steplike discontinuity is always a consequence of the existence of two oscillation states for the same conditions. Stiff materials and small free amplitudes give rise to steplike transitions while the use of large free amplitudes produce smooth transitions. Simulations performed on compliant samples showed cases where the cantilever dynamics is fully controlled by a net attractive force. Phase-shift measurements provide a practical method to determine the operating regime. Finally, we discuss the influence of those regimes in data acquisition and image interpretation.
571 citations
TL;DR: Five different modes of multifrequency force microscopy are reviewed and their application in studies of proteins, the imaging of vibrating nanostructures, measurements of ion diffusion and subsurface imaging in cells is examined.
Abstract: This article reviews the development of multifrequency force microscopy and examines its application in studies of proteins, the imaging of vibrating nanostructures, measurements of ion diffusion, and subsurface imaging in cells.
533 citations
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28 Jul 2005
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。
18,940 citations
TL;DR: The most relevant features of WSXM, a freeware scanning probe microscopy software based on MS-Windows, are described and some relevant procedures of the software are carried out.
Abstract: In this work we briefly describe the most relevant features of WSXM, a freeware scanning probe microscopy software based on MS-Windows. The article is structured in three different sections: The introduction is a perspective on the importance of software on scanning probe microscopy. The second section is devoted to describe the general structure of the application; in this section the capabilities of WSXM to read third party files are stressed. Finally, a detailed discussion of some relevant procedures of the software is carried out.
6,996 citations
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
3,281 citations
TL;DR: The invasion-metastasis cascade is a multistep cell-biological process that involves dissemination of cancer cells to anatomically distant organ sites and their subsequent adaptation to foreign tissue microenvironments as mentioned in this paper.
3,150 citations
TL;DR: Cancer-associated fibroblasts (CAFs) become synthetic machines that produce many different tumour components and have a role in creating extracellular matrix structure and metabolic and immune reprogramming of the tumour microenvironment with an impact on adaptive resistance to chemotherapy.
Abstract: Cancer is associated with fibroblasts at all stages of disease progression. This Review discusses the pleiotropic actions of cancer-associated fibroblasts (CAFs) on tumour cells and postulates that they are likely to be a heterogeneous and plastic population of cells in the tumour microenvironment. Among all cells, fibroblasts could be considered the cockroaches of the human body. They survive severe stress that is usually lethal to all other cells, and they are the only normal cell type that can be live-cultured from post-mortem and decaying tissue. Their resilient adaptation may reside in their intrinsic survival programmes and cellular plasticity. Cancer is associated with fibroblasts at all stages of disease progression, including metastasis, and they are a considerable component of the general host response to tissue damage caused by cancer cells. Cancer-associated fibroblasts (CAFs) become synthetic machines that produce many different tumour components. CAFs have a role in creating extracellular matrix (ECM) structure and metabolic and immune reprogramming of the tumour microenvironment with an impact on adaptive resistance to chemotherapy. The pleiotropic actions of CAFs on tumour cells are probably reflective of them being a heterogeneous and plastic population with context-dependent influence on cancer.
2,597 citations