Showing papers by "Richard A. Jorgensen published in 1981"
TL;DR: Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, the promoter controlling the expression of tetracycline resistance was located and the repressor and the location of its gene was identified.
Abstract: The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.
79 citations
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TL;DR: When Tn10-containing mini-cells were labeled in the presence of Tc a new RNA species was detected that hybridized specifically to DNA sequences from the outer 400 bp of each inverted repeat sequence (IS10).
5 citations
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TL;DR: Tn5 is a transposable genetic element which encodes resistance to aminoglycoside antibiotics such as kanamycin and neomycin and is in general similar to other transposons.
Abstract: Tn5 is a transposable genetic element which encodes resistance to aminoglycoside antibiotics such as kanamycin and neomycin. This resistance results from the synthesis of the enzyme neomycin phosphotransferase type II (NPTII, also named aminoglycoside 3′-phos-photransferase-II) (Berg et al., 1978). Its structure is in general similar to other transposons with two inverted repeats 1534 bp long (Auerswald and Schaller, 1980) flanking a unique central region approximately 2700 bp long (Berg et al., 1975; Jorgensen et al., 1979).
1 citations