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Showing papers by "Richard A. Jorgensen published in 2005"


Journal ArticleDOI
TL;DR: After removing rRNA, vector, bacterial insertion sequence and chimeric cDNA contaminants, small-scale nucleotide discrepancies were found in 51% of cDNA sequences from one Arabidopsis cDNA collection, 89% from a secondArabidopsis collection and 75% fromA rice collection.
Abstract: Summary: Errors are prevalent in cDNA sequences but the extent to which sequence collections differ in frequencies and types of errors has not been investigated systematically. cDNA quality control, or cQC, was developed to evaluate the quality of cDNA sequence collections and to revise those sequences that differ from a higher quality genomic sequence. After removing rRNA, vector, bacterial insertion sequence and chimeric cDNA contaminants, small-scale nucleotide discrepancies were found in 51% of cDNA sequences from one Arabidopsis cDNA collection, 89% from a second Arabidopsis collection and 75% from a rice collection. These errors created premature termination codons in 4 and 42% of cDNA sequences in the respective Arabidopsis collections and in 7% of the rice cDNA sequences. Availability: A web-based version of cQC, source code and revised cDNA collections are available at http://genomics.arizona.edu/software/cQC/ Contact: raj@ag.arizona.edu Supplementary information: Further text, tables and figures are available at the above website or on Bioinformatics online.

4 citations


Journal ArticleDOI
TL;DR: The NIH intends the manuscript repository to provide an alternative means through which NIH grantees can partially fulfill progress reporting requirements and be part of a central resource through which the public may view research funded by U.S. taxpayers.
Abstract: Beginning May 2, 2005, the National Institutes of Health (NIH) requests that authors whose research was supported in whole or in part by NIH deposit their peer-reviewed, accepted manuscripts in PubMed Central, NIH's central repository. The NIH intends the manuscript repository to (1) provide an alternative means through which NIH grantees can partially fulfill progress reporting requirements and (2) be part of a central resource through which the public may view research funded by U.S. taxpayers. The full NIH policy can be viewed at http://grants1.nih.gov/grants/guide/notice-files/NOT-OD-05-022.html.

1 citations