Showing papers by "Richard Lathe published in 1994"
TL;DR: A relative excess of cases with valine at position 129 of the gene's open reading frame is revealed, emphasising the importance of this site of common polymorphism in influencing the neuropathological phenotype in human spongiform encephalopathy.
36 citations
Patent•
21 Apr 1994TL;DR: In this article, an expression unit of an internal ribosome binding site (IRES) coupled to a heterologous gene sequence is described, and this expression unit is bounded at 5' and 3' ends by DNA sequences that enable homologous recombination or integration of the construct with the DNA of a targeted host to obtain expression of the HOG gene in the host.
Abstract: A DNA construct is described containing an expression unit of an internal ribosome binding site (IRES) coupled to a heterologous gene sequence; this expression unit is bounded at 5' and 3' ends by DNA sequences that enable homologous recombination or integration of the construct with the DNA of a targeted host to obtain expression of the heterologous gene in the host.
25 citations
TL;DR: This work focuses on the hippocampus, a brain region involved in learning and memory and an early target for degeneration in Alzheimer's disease, and has yielded transgenic animals with apparent functional deficits in the hippocampus leading the way to a greater understanding of brain function.
Abstract: Many neuropsychiatric disorders have a genetic aetiology. In vivo gene modification offers a route to simulating such disorders in transgenic animals, allowing a systematic study of the underlying pathophysiology. However, attempts to mimic diseases such as Alzheimer's disease in transgenic animals have not yet been successful. This principally reflects our lack of knowledge concerning normal brain function, and an understanding of the biochemical mechanisms underlying cognitive processes is a primary objective. We and others have therefore focused on the hippocampus, a brain region involved in learning and memory and an early target for degeneration in Alzheimer's disease. Genetic intervention to date has yielded transgenic animals with apparent functional deficits in the hippocampus, leading the way to a greater understanding of brain function.
16 citations
Patent•
21 Apr 1994
TL;DR: Une construction d'ADN contient une unite d'expression d'un site de liaison ribosomique interne (IRES) couple a une sequence of genes heterologues du gene heterologue chez l'hote.
Abstract: Une construction d'ADN contient une unite d'expression d'un site de liaison ribosomique interne (IRES) couple a une sequence de genes heterologues; cette unite d'expression est liee aux extremites 5' et 3' par des sequences d'ADN qui permettent une integration ou une recombinaison homologue de la construction avec l'ADN d'un hote cible afin d'obtenir l'expression du gene heterologue chez l'hote.