Robert E. Neuman

TL;DR: A short and simple calorimetric method is here described that is applicable to the determination of hydroxyproline in hydrolysates of 40 to 100 y of collagen with a reproducibility of f2 per cent and an accuracy of f1 per cent as judged by recovery of hydroXYproline from elastin hydrolysate and from an amino acid mixture simulating collagen.

TL;DR: A simple method is described here for determining collagen in small amounts of tissue with a precision and accuracy usually better than f5 per cent and in tissues containing but little collagen much of the soluble protein is separated from the tissues with the aid of 20 per cent urea solution.

TL;DR: Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.

TL;DR: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds and its application in drug discovery is described.

TL;DR: A critical review is given of hydroxyproline analysis, finding a procedure which is faster and more sensitive than the former one and as reliable.