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Robert G.J. Vries
Researcher at University Medical Center Utrecht
Publications - 45
Citations - 15865
Robert G.J. Vries is an academic researcher from University Medical Center Utrecht. The author has contributed to research in topics: Stem cell & Organoid. The author has an hindex of 25, co-authored 34 publications receiving 12514 citations. Previous affiliations of Robert G.J. Vries include Leiden University Medical Center & Utrecht University.
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Journal ArticleDOI
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Toshiro Sato,Robert G.J. Vries,Hugo J. Snippert,Marc van de Wetering,Nick Barker,Daniel E. Stange,Johan H. van Es,Arie Abo,Pekka Kujala,Peter J. Peters,Hans Clevers +10 more
TL;DR: It is concluded that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
Journal ArticleDOI
Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
Journal ArticleDOI
Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Toshiro Sato,Johan H. van Es,Hugo J. Snippert,Daniel E. Stange,Robert G.J. Vries,Maaike van den Born,Nick Barker,Noah F. Shroyer,Marc van de Wetering,Hans Clevers +9 more
TL;DR: It is concluded that Lgr5 stem cells compete for essential niche signals provided by a specialized daughter cell, the Paneth cell, in colon crypts, and co-culturing of sorted stem cells with Paneth cells markedly improves organoid formation.
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In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Meritxell Huch,Craig Dorrell,Sylvia F. Boj,Johan H. van Es,Vivian S. W. Li,Marc van de Wetering,Toshiro Sato,Toshiro Sato,Karien Hamer,Nobuo Sasaki,Milton J. Finegold,Annelise Haft,Robert G.J. Vries,Markus Grompe,Hans Clevers +14 more
TL;DR: Findings indicate that previous observations concerning Lgr5+ stem cells in actively self-renewing tissues can also be extended to damage-induced stem Cells in a tissue with a low rate of spontaneous proliferation.
Journal ArticleDOI
Tissue-specific mutation accumulation in human adult stem cells during life
Francis Blokzijl,Joep de Ligt,Myrthe Jager,Valentina Sasselli,Sophie Roerink,Nobuo Sasaki,Meritxell Huch,Sander Boymans,Ewart W. Kuijk,Pjotr Prins,Isaac J. Nijman,Inigo Martincorena,Michal Mokry,Caroline L. Wiegerinck,Sabine Middendorp,Toshiro Sato,Gerald Schwank,Edward E. S. Nieuwenhuis,Monique M A Verstegen,Luc J. W. van der Laan,Jeroen de Jonge,Jan N. M. IJzermans,Robert G.J. Vries,Marc van de Wetering,Michael R. Stratton,Hans Clevers,Edwin Cuppen,Ruben van Boxtel +27 more
TL;DR: This work determines genome-wide mutation patterns in ASCs of the small intestine, colon and liver of human donors with ages ranging from 3 to 87 years by sequencing clonal organoid cultures derived from primary multipotent cells.