Author
Roberto Romero
Other affiliations: University of Michigan, Weizmann Institute of Science, University of Tennessee Medical Center ...read more
Bio: Roberto Romero is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Amniotic fluid & Chorioamnionitis. The author has an hindex of 151, co-authored 1516 publications receiving 108321 citations. Previous affiliations of Roberto Romero include University of Michigan & Weizmann Institute of Science.
Topics: Amniotic fluid, Chorioamnionitis, Pregnancy, Amniocentesis, Preeclampsia
Papers published on a yearly basis
Papers
More filters
••
TL;DR: Galectin-1 mRNA and protein were detected in amniotic epithelium, chorioamniotic mesodermal cells, and decidual stromal cells.
Abstract: Problem Galectin-1 can regulate immune responses upon infection and inflammation. We determined galectin-1 expression in the chorioamniotic membranes and its changes during histological chorioamnionitis.
Method of study Chorioamniotic membranes were obtained from women with normal pregnancy (n = 5) and from patients with pre-term pre-labor rupture of the membranes (PPROM) with (n = 8) and without histological chorioamnionitis (n = 8). Galectin-1 mRNA and protein were localized by in situ hybridization and immunohistochemistry. Galectin-1 mRNA expression was also determined by quantitative reverse transcriptase polymerase chain reaction.
Results Galectin-1 mRNA and protein were detected in the amniotic epithelium, chorioamniotic fibroblasts/myofibroblasts and macrophages, chorionic trophoblasts, and decidual stromal cells. In patients with PPROM, galectin-1 mRNA expression in the fetal membranes was higher (2.07-fold, P = 0.002) in those with chorioamnionitis than in those without. Moreover, chorioamionitis was associated with a strong galectin-1 immunostaining in amniotic epithelium, chorioamniotic mesodermal cells, and apoptotic bodies.
Conclusion Chorioamnionitis is associated with an increased galectin-1 mRNA expression and strong immunoreactivity of the chorioamniotic membranes; thus, galectin-1 may be involved in the regulation of the inflammatory responses to chorioamniotic infection.
32 citations
••
TL;DR: Circulating early transcriptomic markers for preeclampsia can be found either by untargeted profiling of the cellular transcriptome or by focusing on placental cell-specific mRNAs.
Abstract: To determine whether previously established mRNA signatures are predictive of early preeclampsia when evaluated by maternal cellular transcriptome analysis in samples collected before clinical mani...
32 citations
••
TL;DR: Vaisbuch et al. as discussed by the authors found that high concentrations of fragment Bb (FBb) in maternal blood, as early as the first trimester, are associated with subsequent spontaneous preterm delivery <34 weeks of gestation.
Abstract: Citation Vaisbuch E, Romero R, Erez O, Mazaki-Tovi S, Kusanovic JP, Soto E, Dong Z, Chaiworapongsa T, Kim SK, Ogge G, Pacora P, Yeo L, Hassan SS. Activation of the alternative pathway of complement is a feature of pre-term parturition but not of spontaneous labor at term. Am J Reprod Immunol 2010; 63: 318–330
Problem Plasma concentrations of fragment Bb (FBb) are a marker for activation of the alternative pathway of the complement system. High concentrations of FBb in maternal blood, as early as the first trimester, are associated with subsequent spontaneous pre-term delivery <34 weeks of gestation. The aim of this study was to determine whether spontaneous pre-term labor (PTL) with intact membranes, intra-amniotic infection/inflammation (IAI) or labor at term are associated with alterations in circulating maternal FBb concentrations.
Method of study This cross-sectional study included women in the following groups: (i) non-pregnant (n = 40); (ii) normal pregnancy (gestational age range 20–36, 6/7 weeks, n = 63); (iii) women at term not in labor (n = 70); (iv) women at term in spontaneous labor (n = 59); (v) patients with an episode of PTL who delivered at term (n = 62); (vi) PTL without IAI who delivered pre-term (n = 30); and (vii) PTL with IAI who delivered pre-term (n = 67). Maternal plasma FBb concentrations were determined by ELISA.
Results (i) Among patients with PTL, those who had a pre-term delivery either with IAI (1.21 μg/mL, IQR 0.77–2.16) or without IAI (1.13 μg/mL, IQR 0.92–2.08) had a higher median maternal plasma FBb concentration than those who delivered at term (0.86 μg/mL, IQR 0.64–1.57; P = 0.007 and P = 0.026, respectively); (ii) there was no difference in the median plasma FBb concentration between patients with and without IAI who delivered pre-term (P = 0.9); (iii) in contrast, spontaneous labor at term was not associated with a significant change in the maternal plasma FBb concentration (P = 0.8); (iv) maternal plasma concentration of FBb did not differ significantly between normal pregnant women and the non-pregnant controls (P = 0.8) and were not correlated with advancing gestational age (r = −0.28, P = 0.8).
Conclusion (i) Pre-term parturition is associated with activation of the alternative complement pathway in maternal circulation; (ii) such activation is not detectable in spontaneous labor at term; (iii) IAI does not explain the activation of the alternative pathway of complement in PTL. Collectively, these observations suggest that pre-term and term labors have fundamental differences in the regulation of innate immunity.
32 citations
••
TL;DR: In this paper, the frequency of adverse perinatal outcome in women with hyperemesis gravidarum and identify prognostic factors were determined by comparing the clinical profile of patients with HG with a normal and an adverse pregnancy outcome.
32 citations
••
01 Dec 2011
TL;DR: Obstetric Complications Breech Presentation, M.P. O'Grady, and C. McIljargie Cesarean Section and Vaginal birth after Cesareans Section, E.N. Winn Gestational Hypertension/Pre-Eclampsia, B.C. Winn and R.R. Romero Invasive Hemodynamic Monitoring in Obstetrics, G.G. Petrie Coagulopathies in obstetrics.
Abstract: Obstetric Complications Breech Presentation, M.L. Gimovsky, J.P. O'Grady, and C. McIljargie Cesarean Section and Vaginal birth after Cesarean Section, E.P. Schneider, G. Farmikides, and H.N. Winn Gestational Hypertension/Pre-Eclampsia, B.M Sibai and A.D. Knoury Incompetent Cervix, A.I. Kivikoski Multiple Gestation, H.N. Winn and W.R. Gerber Abruptio Placentae and Placenta Previa, C.C. Egley Post-Term Pregnancy, H.N. Winn Premature Labor, E. Amon Premature Rupture of the Fetal Membranes, A. Al-Malt Recurrent Pregnancy Losses, C.B. Coulan Obstetrical Emergencies Amniotic Fluid Embolism, H.N. Winn and R.H. Petrie Coagulopathies in Obstetrics, H.N. Winn and R. Romero Invasive Hemodynamic Monitoring in Obstetrics, G.D.V. Hankins Postpartum Hemorrhage, P. Tropper Septic shock, P. Duff and K.M. Davidson Shoulder Dystocia, J.S. Smeltzer Sickle Cell Crises, J.C. Morrison Perinatal Infections - Bacterial Infections Gonorrhea Infection, J.B. Shumway Group B streptococcus Infection, H.N Winn and H. Hamill Other Bacterial Infections: Listeria, Lyme Disease, Granuloma Inguinale and Chancroid, J.L. Swingler Postpartum Infections, D.J. Mostello Syphilis Infection, J.B. Shumway Urinary Tract Infection, S.R. Allen Perinatal Infections - Viral Infections Cytomegaloviruses, C.R.G. Monif Herpes Infection: Herpes Simplex Virus and Varicella Zoster Virus, R.R. Vicarello Human Immunodeficiency Virus, S.A. Gall Other Viral Infections, S.A. Gall Perinatal Infections - Chlamydia Infection Chlamydia Infection, J.B. Shumway Perinatal Infections - Protozoan Infection Protozoan Infection: Toxoplasmosis, Trichomoniasis, Lice and Scabies, E.R. Newton Perinatal Infections - Other Infections and Issues Bacterial Vaginosis in Pregnancy: Evidence-Based Approaches, J.A. McGregor and J.I. French Candidiasis and Helminthic Infection, J.M. Piper and E.R. Newton Immunizations in Pregnancy, J.E. Deaver Maternal Medical Disorders Cardiac Diseases, P. Cole Dermatologic Diseases, A.G. Martin and S. Leal-Khouri Diabetes Mellitus, D.R. Coustan Endocrine Diseases, W.E. Clutter Gastrointestinal Diseases, D.C. Rubin Hematological Diseases, M.A. Blinder Hepatic Diseases, H.M. White and M.G. Peters Hypertension, W.F. Rayburn Neurologic Diseases, D.B. Clifford Psychiatric Diseases, M. Artal Pulmonary Diseases,D. Schuller and D.P. Schuster Renal Diseases, F. Horvath Rheumatological Diseases and Antiphospholipid Syndrome, A.T. Masi, S.L. Feigenbaum, and M.D. Lockshin Maternal Surgical Disorders Acute Abdomen in Pregnancy, W.L. Holcomb, Jr. Non-Obstetrical Surgery During Pregnancy: Medical Evaluation and Management, M. Pietrantoni, S. Sawai, and R.A. Knuppel Trauma in Pregnancy, R. Hayashi Fetal Disorders Alloimmune Thrombocytopenia, A.B. Levine and R.L. Berkowitz Antepartum Fetal Heart Rate Monitoring, M.L. Druzin Doppler Velocimetry for Fetal Surveillance: Principles and Practice, D. Maulik Erythroblastosis Fetalis, P.N. Rauk and A. Daftary Fetal Age Assessment, C.M. Martin Fetal Anomalies, H.N. Winn Fetal Biophysical Profile, A.M. Vintzileos, W.A. Campbell, and J.F. Rodis Fetal Blood Sampling, F. Daffos Fetal Demise, J.M. Shyken Fetal Echocardiography: Prenatal Diagnosis of Congenital Heart Disease, G. Pilu, S. Gabrielli, A. Perolo, and D. Prandstraller Fetal Growth Restriction, M.Y. Divon Fetal Lung Maturity, J.A. Bartelsmeyer Fetal Macrosomia, H.N. Winn Fetal Oxygenation and Acidosis, H.N. Winn and R.H. Petrie Nonimmune Fetal Hydrops, H.N. Winn Perinatal Genetics Basic genetics and Patterns of Inheritance, D.K. Grange Fetal Genetic Disorders, J. Pratt Rossiter and K.J. Blakemore Maternal Serum a-Fetoprotein Screening, B.K. Burton Drug Abuse Principles of Teratology of Drugs and Radiation, F.R. Witter Drug Abuse in Pregnancy: Hallucinogens, Stimulants, Alcohol and Opiates, J.C. Howitt Other Maternal/Perinatal Issues Amnioinfusion, E. Amon, J. Kerns, and H.N. Winn Anesthesia and Analgesia in Pregnancy, G.A. Albright, R.M. Forster, and P.J. Bolster Exercise and Pregnancy, R. Artal Mature Gravidas, C.C. Egley Neonatal Diseases I, W.J. Keenan and S.S. Toce Neonatal Diseases II, J.G. Dawson, J.L. Rosenbaum, and F. Sessions Cole Neoplasia and Pregnancy, T.J. Herzog and D.G. Mutch Placental Transport, Metabolism and Perinatal Nutrition, J.M. Dicke What is Obstetric Ethics? F.A. Chervanak and L.B. McCullough Index
32 citations
Cited by
More filters
••
TL;DR: The philosophy and design of the limma package is reviewed, summarizing both new and historical features, with an emphasis on recent enhancements and features that have not been previously described.
Abstract: limma is an R/Bioconductor software package that provides an integrated solution for analysing data from gene expression experiments. It contains rich features for handling complex experimental designs and for information borrowing to overcome the problem of small sample sizes. Over the past decade, limma has been a popular choice for gene discovery through differential expression analyses of microarray and high-throughput PCR data. The package contains particularly strong facilities for reading, normalizing and exploring such data. Recently, the capabilities of limma have been significantly expanded in two important directions. First, the package can now perform both differential expression and differential splicing analyses of RNA sequencing (RNA-seq) data. All the downstream analysis tools previously restricted to microarray data are now available for RNA-seq as well. These capabilities allow users to analyse both RNA-seq and microarray data with very similar pipelines. Second, the package is now able to go past the traditional gene-wise expression analyses in a variety of ways, analysing expression profiles in terms of co-regulated sets of genes or in terms of higher-order expression signatures. This provides enhanced possibilities for biological interpretation of gene expression differences. This article reviews the philosophy and design of the limma package, summarizing both new and historical features, with an emphasis on recent enhancements and features that have not been previously described.
22,147 citations
••
TL;DR: The latest version of STRING more than doubles the number of organisms it covers, and offers an option to upload entire, genome-wide datasets as input, allowing users to visualize subsets as interaction networks and to perform gene-set enrichment analysis on the entire input.
Abstract: Proteins and their functional interactions form the backbone of the cellular machinery. Their connectivity network needs to be considered for the full understanding of biological phenomena, but the available information on protein-protein associations is incomplete and exhibits varying levels of annotation granularity and reliability. The STRING database aims to collect, score and integrate all publicly available sources of protein-protein interaction information, and to complement these with computational predictions. Its goal is to achieve a comprehensive and objective global network, including direct (physical) as well as indirect (functional) interactions. The latest version of STRING (11.0) more than doubles the number of organisms it covers, to 5090. The most important new feature is an option to upload entire, genome-wide datasets as input, allowing users to visualize subsets as interaction networks and to perform gene-set enrichment analysis on the entire input. For the enrichment analysis, STRING implements well-known classification systems such as Gene Ontology and KEGG, but also offers additional, new classification systems based on high-throughput text-mining as well as on a hierarchical clustering of the association network itself. The STRING resource is available online at https://string-db.org/.
10,584 citations
01 Jun 2012
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Abstract: The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.
10,124 citations
01 Jan 2014
TL;DR: These standards of care are intended to provide clinicians, patients, researchers, payors, and other interested individuals with the components of diabetes care, treatment goals, and tools to evaluate the quality of care.
Abstract: XI. STRATEGIES FOR IMPROVING DIABETES CARE D iabetes is a chronic illness that requires continuing medical care and patient self-management education to prevent acute complications and to reduce the risk of long-term complications. Diabetes care is complex and requires that many issues, beyond glycemic control, be addressed. A large body of evidence exists that supports a range of interventions to improve diabetes outcomes. These standards of care are intended to provide clinicians, patients, researchers, payors, and other interested individuals with the components of diabetes care, treatment goals, and tools to evaluate the quality of care. While individual preferences, comorbidities, and other patient factors may require modification of goals, targets that are desirable for most patients with diabetes are provided. These standards are not intended to preclude more extensive evaluation and management of the patient by other specialists as needed. For more detailed information, refer to Bode (Ed.): Medical Management of Type 1 Diabetes (1), Burant (Ed): Medical Management of Type 2 Diabetes (2), and Klingensmith (Ed): Intensive Diabetes Management (3). The recommendations included are diagnostic and therapeutic actions that are known or believed to favorably affect health outcomes of patients with diabetes. A grading system (Table 1), developed by the American Diabetes Association (ADA) and modeled after existing methods, was utilized to clarify and codify the evidence that forms the basis for the recommendations. The level of evidence that supports each recommendation is listed after each recommendation using the letters A, B, C, or E.
9,618 citations
••
TL;DR: A short cervical length and a raised cervical-vaginal fetal fibronectin concentration are the strongest predictors of spontaneous preterm birth.
6,275 citations