Showing papers by "Ronald Klein published in 1980"
TL;DR: This study compared hyperglycemic streptozocin- and insulin-treated or non-insulin-treated rats, hyperglycemia rats with pancreatectomy, and normal rats to determine whether elevated vitreous fluorescence levels are a result of a direct ocular toxic effect of streptzocin or a consequence of the diabetic state.
Abstract: • To determine whether elevated vitreous fluorescence levels are a result of a direct ocular toxic effect of streptozocin or a consequence of the diabetic state, we compared hyperglycemic streptozocinand insulin-treated or non-insulintreated rats, hyperglycemic rats with pancreatectomy, and normal rats. Streptozocin, 65 mg/kg, was injected intravenously in one group of hooded rats, and pancreatectomy was performed on another group of hooded rats. Eight to 16 days later, fluorescein sodium, 16.6 mg/kg, was injected in the femoral vein of 21 hyperglycemic rats and 18 normal animals. One hour later, the aqueous, vitreous, and plasma fluorescence levels were measured using fluorophotometry. We did not find a significant difference between vitreous fluorescence levels of the hyperglycemic streptozocin- and insulintreated or non-insulin-treated and normal rats. Moreover, we did not find a significant difference between the vitreous fluorescence of hyperglycemic rats with pancreatectomy and normal rats.
20 citations
TL;DR: Using an artificial eye and a cat eye that had undergone vitrectomy, it is found that scatter of light from the retinal surface may contribute to the fluorescence recorded.
Abstract: • A radiometer and photomultiplier assembly was used to measure light intensities under several artificial conditions to assess the efficacy of the fluorophotometer and the technique of ocular fluorophotometry. Using an artificial eye and a cat eye that had undergone vitrectomy, we found that scatter of light from the retinal surface may contribute to the fluorescence recorded.
19 citations
TL;DR: The excessive fluorescence of the vitreous is similar to that reported by others in diabetic humans and rats but is found in the guinea pig, an animal known normally to have no retinal vasculature.
Abstract: • Normal and streptozocin-treated guinea pigs were examined with a fluorophotometer one hour after receiving an intravenous injection of fluorescein sodium. The level of fluorescence in the vitreous was significantly greater in animals showing marked glucosuria than in normal animals or those showing little glucosuria. The excessive fluorescence of the vitreous is similar to that reported by others in diabetic humans and rats but is found in the guinea pig, an animal known normally to have no retinal vasculature.
18 citations