Author
Ronald L. Searcy
Other affiliations: Scott & White Hospital
Bio: Ronald L. Searcy is an academic researcher from University of California, Los Angeles. The author has contributed to research in topics: Amylase & Serum albumin. The author has an hindex of 13, co-authored 34 publications receiving 1287 citations. Previous affiliations of Ronald L. Searcy include Scott & White Hospital.
Topics: Amylase, Serum albumin, Color reaction, Blood serum, Cholesterol
Papers
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TL;DR: The serum total and free cholesterol levels of 200 blood donors measured with the new color reaction were found to agree well with normal values reported by others and validates its use for either clinical or research purposes.
556 citations
221 citations
TL;DR: In this article, an ultramicro cholesterol technique based upon a new color reaction and requiring only 10 μl of serum is proposed, where the need for extraction with lipid solvent is eliminated by drying serum on filter paper segments and eluting directly in the ferrous sulfate color reagent.
132 citations
TL;DR: Fractionations suggest that a majority, if not all, saccharogenic activity in normal and certain abnormal specimens is chromatographically separable from other serum protein and exhibits an electrophoretic mobility similar to γ-globulin.
Abstract: The dinitrosalicylic acid reaction for measuring reducing sugar has been evaluated and found suitable for determining the saccharogenic activity of human serum. The sensitivity and reproducibility of the modified dinitrosalicylic acid method permits quantitation of the electrophoretic and chromatographic distribution of human serum amylase. Such fractionations suggest that a majority, if not all, saccharogenic activity in normal and certain abnormal specimens is chromatographically separable from other serum protein and exhibits an electrophoretic mobility similar to γ-globulin.
54 citations
TL;DR: Evaluation of the Berthelot colour reaction has shown that reagent sequence is an important determinant of the chromogenic response and provides a basis for measuring urea nitrogen concentration of serum or protein-free filtrates with a degree of reliability satisfactory for most routine or research purposes.
43 citations
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3,508 citations
TL;DR: Catalyzed phenol-hypochlorite and ninhydrin colorimetric procedures were adapted to the Technicon AutoAnalyzer for simultaneous determination of ammonia and total amino acids in ruminal fluid or ruminal in vitro media and indicated high degrees of accuracy and precision for both ammonia and amino acid analyses.
1,806 citations
TL;DR: The role and identity of the "catalyst" for the Berthelot reaction are examined in detail in detail as mentioned in this paper, and a new procedure for ammonia analysis which is superior in both sensitivity and precision to the other published procedures is presented.
Abstract: An investigation of the mechanism of the Berthelot reaction has led to an increased understanding of the many conflicts in the literature concerning the reaction's use for the analytical determination of ammonia. Monochloramine is shown to be the first intermediate, and the kinetics of its formation and decomposition are presented. The identical visible spectra obtained for a series of indophenols synthesized in the course of this research and the blue complexes formed when phenol or a substituted phenol was used in the Berthelot reaction confirmed that indophenol is the final product of the reaction. Conditions which lead to the formation of final products other than indophenol have been observed and are also discussed. The role and identity of the ''catalyst'' for the Berthelot reaction are examined in detail. Finally, a new procedure for ammonia analysis which is superior in both sensitivity and precision to the other published procedures is presented.
754 citations
TL;DR: In this article, an automated technique was developed for estimating NH4+ in Kjeldahl digests of crops, using the salicylate-dichloroisocyanurate reaction in the presence of nitroprusside.
Abstract: An automated technique has been developed for estimating NH4+ in Kjeldahl digests of crops, using the salicylate-dichloroisocyanurate reaction in the presence of nitroprusside. The method gives reproducible results in close agreement with those from conventional distillation and titration, and there is a marked improvement in output.
722 citations
TL;DR: Total ammonia-nitrogen (NH4-N) in artificial seawater was determined spectrophotometrically by a method in which indophenol blue is produced by the reaction of ammonia with salicylate and hypochlor...
Abstract: Total ammonia-nitrogen (NH4-N) in artificial seawater was determined spectrophotometrically by a method in which indophenol blue is produced by the reaction of ammonia with salicylate and hypochlor...
672 citations