Author
Rupinder Tewari
Other affiliations: Washington University in St. Louis
Bio: Rupinder Tewari is an academic researcher from Panjab University, Chandigarh. The author has contributed to research in topics: Phosphate solubilizing bacteria & Mycobacterium tuberculosis. The author has an hindex of 28, co-authored 88 publications receiving 4050 citations. Previous affiliations of Rupinder Tewari include Washington University in St. Louis.
Papers published on a yearly basis
Papers
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TL;DR: This review discusses various types of pectinases and their applications in the commercial sector.
1,001 citations
TL;DR: This review discusses the occurrence and structure of chitin, the types and sources of ch itinases, their mode of action, chit inase production, as well as molecular cloning and protein engineering of Chitinases and their biotechnological applications.
Abstract: Chitin and chitinases (EC 3.2.1.14) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosaccharides and N-acetyl D-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and yeast, control of pathogenic fungi, treatment of chitinous waste, and control of malaria transmission. In this review, we discuss the occurrence and structure of chitin, the types and sources of chitinases, their mode of action, chitinase production, as well as molecular cloning and protein engineering of chitinases and their biotechnological applications.
499 citations
TL;DR: The present review features the potential applications and uses of microbial alkaline pectinases, the nature of pECTin, and the vast range of pectinolytic enzymes that function to mineralize pectic substances present in the environment to explore the potential of these enzymes and to encourage new alkalinepectinase-based industrial technology.
Abstract: The biotechnological potential of pectinolytic enzymes from microorganisms has drawn a great deal of attention from various researchers worldwide as likely biological catalysts in a variety of industrial processes. Alkaline pectinases are among the most important industrial enzymes and are of great significance in the current biotechnological arena with wide-ranging applications in textile processing, degumming of plant bast fibers, treatment of pectic wastewaters, paper making, and coffee and tea fermentations. The present review features the potential applications and uses of microbial alkaline pectinases, the nature of pectin, and the vast range of pectinolytic enzymes that function to mineralize pectic substances present in the environment. It also emphasizes the environmentally friendly applications of microbial alkaline pectinases thereby revealing their underestimated potential. The review intends to explore the potential of these enzymes and to encourage new alkaline pectinase-based industrial technology.
421 citations
TL;DR: Of all the substrates tested, wheat bran appeared to be the best suited substrate producing appreciable yields of CMCase, FPase and β-glucosidase at the levels of 310, 17 and 33 U/g dry substrate respectively.
234 citations
TL;DR: A soil isolate, Bacillus sp.
Abstract: A soil isolate, Bacillus sp. DT7 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as pectin lyase (EC 4.2.2.10). By optimizing growth conditions, Bacillus sp. DT7 produced higher amount of pectin lyase (53 units/ml) than that has been reported in the literature. Using gel filtration and ion exchange chromatography, this enzyme was purified and found to have a molecular mass of 106 kDa. The purified enzyme exhibited maximal activity at a temperature of 60 ∘C and pH 8.0. The presence of 100 mM concentrations of CaCl2 and mercaptoethanol significantly enhanced pectinase activity of the purified enzyme. This pectinase has tremendous applications in textile industry, plant tissue maceration and fruit juice wastewater treatments.
163 citations
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TL;DR: Methods of molecular-specific diagnostics/detection of cancer, including strongly enhanced surface plasmon resonance light-scattering, surface-enhanced emission of gold nanorods and surface- enhanced Raman scattering, are described.
1,333 citations
TL;DR: The introduction of azido functions in chitosan has provided photo-sensitive hydrogels for surgical use as coatings, scaffolds, drug carriers and implants capable to deliver cells and growth factors, which remain unmatched by other polysaccharides.
1,003 citations
TL;DR: This review discusses various types of pectinases and their applications in the commercial sector.
1,001 citations
TL;DR: Pectinases are one of the most widely distributed enzymes in bacteria, fungi and plants as discussed by the authors, and they have a share of 25% in the global sales of food enzymes.
975 citations