R
Russell Higuchi
Researcher at Cetus Corporation
Publications - 24
Citations - 25630
Russell Higuchi is an academic researcher from Cetus Corporation. The author has contributed to research in topics: Polymerase chain reaction & DNA polymerase. The author has an hindex of 9, co-authored 22 publications receiving 25346 citations. Previous affiliations of Russell Higuchi include Hoffmann-La Roche.
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
TL;DR: The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.
Journal ArticleDOI
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions
TL;DR: These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specific interactions of proteins and DNA.
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DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
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Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA
Corinne Wong,Carol E. Dowling,Randall Keichi Saiki,Russell Higuchi,Henry A. Erlich,Haig H. Kazazian +5 more
TL;DR: This work studied the genomic DNA of five patients with β-thalassaemia, and found two previously undescribed mutations, along with three known alleles, including the first natural mutation observed at the cap site of the β-globin gene.