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Author

S. Miyamura

Bio: S. Miyamura is an academic researcher. The author has contributed to research in topics: Biosensor & Medicine. The author has co-authored 6 publications.

Papers
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25 Apr 2022
TL;DR: This dual-comb biosensing technique has the potential to reduce the COVID-19 testing time to 10 min while maintaining sensitivity close to that of RT-PCR and can be applied for sensing of not only viruses but also various biomolecules for clinical diagnosis, health care, and environmental monitoring.
Abstract: Rapid, sensitive detection of biomolecules is important for improved testing methods for viruses as well as biomarkers and environmental hormones. For example, testing for SARS-CoV-2 is essential in the fight against the COVID-19 pandemic. Reverse-transcription polymerase chain reaction (RT-PCR) is the current standard for COVID-19 testing; however, it is hampered by the long testing process. Shortening the testing process while achieving high sensitivity would facilitate sooner quarantine and thus presumably prevention of the spread of SARS-CoV-2. Here, we aim to achieve rapid, sensitive detection of the SARS-CoV-2 nucleocapsid protein by enhancing the performance of optical biosensing with a dual-comb configuration of optical frequency combs. The virus-concentration-dependent optical spectrum shift is transformed into a photonic RF shift by frequency conversion between the optical and RF regions, facilitating mature electrical frequency measurements. Furthermore, active-dummy temperature-drift compensation enables very small changes in the virus-concentration-dependent signal to be extracted from the large, variable background signal. This dual-comb biosensing technique has the potential to reduce the COVID-19 testing time to 10 min while maintaining sensitivity close to that of RT-PCR. Furthermore, this system can be applied for sensing of not only viruses but also various biomolecules for clinical diagnosis, health care, and environmental monitoring.
Proceedings ArticleDOI
06 Mar 2023
TL;DR: In this paper , the authors demonstrated detection of SARS-CoV-2 antigen based on a combination of dual fiber combs, an intracavity multi-mode-interference fiber sensor, and sensor surface modification.
Abstract: One interesting feature of optical frequency comb (OFC) is a function of frequency conversion between region and electric regions. While such feature has been used for generation of correct electric signal in microwave or millimeter region, it can be further used for fiber biosensing; namely, biosensing OFC. In this paper, we demonstrated detection of SARS-CoV-2 antigen based on a combination of dual fiber combs, an intracavity multi-mode-interference fiber sensor, and sensor surface modification of SARS-CoV-2 antibody.
TL;DR: The optical biosensing based on optical frequency comb (OFC), enabling the rapid detection of SARS-CoV-2 nucleocapsid protein, has a potential to reduce the testing time down of COVID-19 to a few tens minute, which is one order of magnitude shorter than that of RT-PCR.
Abstract: Testing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential along with vaccination and inactivation to fight against the coronavirus disease 2019 (COVID-19) pandemic. Reverse-transcription polymerase chain reaction (RT-PCR), based on reverse transcription of RNA into DNA and amplification of specific DNA targets, is the current standard for COVID-19 testing;however, it hampers from laborious and time-consuming multiple steps. If the testing is largely simplified and shortened, it will be a powerful deterrent to the spread of COVID-19. Here we demonstrate the optical biosensing based on optical frequency comb (OFC), enabling the rapid detection of SARS-CoV-2 nucleocapsid protein. The virus-concentration-dependent optical spectral shift caused by antigen-antibody interaction and multimode-interference fiber sensor is transformed into a photonic radio-frequency (RF) shift by coherent frequency link between optical and RF regions in OFC, benefiting from high precision, rapid, simple, and low cost in electric frequency measurements. Furthermore, the active-dummy compensation of temperature drift with dual-comb configuration extracts the imperceptible change of virus-concentration-dependent signal from the large background signal that changes moment by moment. Such the dual-comb biosensing has a potential to reduce the testing time down of COVID-19 to a few tens minute, which is one order of magnitude shorter than that of RT-PCR (typically, 5 hours). Furthermore, it will be applied for sensitive sensing of not only virus of emerging and re-emerging infectious diseases but also RNA, bio-marker, and endocrine disruptor by selecting the surface modification of biomolecule interaction.
Proceedings ArticleDOI
01 May 2022
TL;DR: This work demonstrated rapid detection of SARS-CoV-2 nucleocapsid protein antigen by dual-comb biosensing with surface modification of its corresponding antibody with a sensitivity close to that of RT-PCR.
Abstract: We demonstrated rapid detection of SARS-CoV-2 nucleocapsid protein antigen by dual-comb biosensing with surface modification of its corresponding antibody. A sensitivity close to that of RT-PCR was achieved, thanks to the use of active–dummy temperature compensation.
Proceedings ArticleDOI
01 May 2022
TL;DR: In this paper , the authors suppress the temperature drift in the refractive index-sensing optical frequency comb (RI-Sensing OFC) by taking the difference of repetition frequency between an active RI-Sensing OFC and a dummy one in the mechanical-sharing dual-fiber-cavity configuration.
Abstract: We suppress the temperature drift in the refractive-index-sensing optical frequency comb (RI-sensing OFC) by taking the difference of repetition frequency between an active RI-sensing OFC and a dummy one in the mechanical-sharing dual-fiber-cavity configuration.