S. P. Basu

Bio: S. P. Basu is an academic researcher from Birla Institute of Technology, Mesra. The author has an hindex of 1, co-authored 1 publications receiving 2 citations.

Chemical Investigations of the Sapindus mukorossi Seed Oil

Abstract: Two lipid fractions ‘A’ and ‘B’ were isolated from Sapindus mukorossi seed oil by preparative TLC. Fraction ‘A’ (70.4%, Rf value 0.76) is a normal triglyceride and its fatty acid compositions was determined by GLC. Fraction ‘B’ (29.6%, Rf value 0.51) shows the presence of nitrogenous constituents. It develops a reddish brown colour in contact with alkali or alkoxide solution. Percentages of individual acids present in fraction ‘A’ were found to be: palmitic, 5.5; stearic, 3.2; oleic, 64.6; linoleic, 2.9; arachidic, 3.1; eicosenoic, 20.1; minor acids, 0.6. Fraction ‘A’ is composed of 0.1,3.6,29.9 and 66.4 percent trisaturated, monounsaturated-disaturated, diunsaturated-monosaturated and triunsaturated glycerides respectively. On GLC analysis, the percentages of individual acids constituting fraction ‘B’ were found to be:palmitic, 3.8; stearic, 1.5; oleic, 33.6; linoleic, 2.9; arachidic, 11.1; eicosenoic, 30.2; behenic, 2.8; docosenoic, 1.4 and two unidentified acids 7.3 and 5.4. Fraction ‘B’ responded to hydrolysis by pancreatic lipase and the product with polarity equivalent to that of 2-mono-glyceride was isolated by TLC and converted to methyl ester. Percentages of individual acids constituting that methyl esters were found to be:palmitic, 11.8; stearic, 4.1; oleic, 12.8; linoleic, 3.7; arachidic, 8.3; eicosenoic, 10.2; behenic, 6.5; docosenoic, 4.2; and two unidentified acids 22.2 and 16.2. This non-glyceridic component of the S. mukorossi seed oil is a cyanolipid, 1-cyano-2-hydroxymethyl prop-1-ene-3-ol. The structure was confirmed by I. R., N.M.R. and Mass spectral-analysis. Chemische Untersuchungen des Saatols Sapindus mukorossi Zwei Lipidfraktionen ‚A’ und ‚B’ wurden durch praparative DC aus dem Saatol Sapindus mukorossi isoliert. Fraktion ‚A’ (70.4%, Rf-Wert 0.76) ist ein normales Triglycerid. Seine Fettsaure-Zusammensetzung wurde durch GC bestimmt. Fraktion ‚B’ (29.6%, Rf-Wert 0.51) zeigt die Gegenwart von stickstoffhaltigen Bestandteilen. Es entwickelt bei Kontakt mit Alkali oder Alkyloxidlosung eine rotlich braune Farbe. Die prozentualen Anteile in der Fraktion ‚A’ betrugen: Palmitinsaure 5.5%, Stearinsaure 3.2%, Olsaure 64.6%, Linolsaure 2.9%, Arachidonsaure 3.1%, Eicosensaure 20.1%, andere Sauren 0.6%, Fraktion ‚A’ ist aus jeweils 0.1,3.6,29.9 und 66.4% dreifachgesattigten, einfachungesattigt-zweifachgesattigten, zweifachungesattigt-einfachgesattigten und dreifachungesattigten Glyceriden zusammengesetzt. Durch GC-Analyse wurde der prozentuale Anteil der einzelnen Sauren, aus denen Fraktion ‚B’ besteht, wie folgt ermittelt: Palmitinsaure 3.8%, Stearinsaure 1.5%, Olsaure 33.6%, Linolsaure 2.9%, Arachidonsaure 11.1%, Eicosensaure 30.2%, Behensaure 2.8%, Docosensaure 1.4% und zwei unidentifizierte Sauren 7.3% und 5.4%, Fraktion ‚B’ reagiert auf Hydrolyse durch Pankreaslipase. Das Produkt mit einer gleichen Polaritat wie 2-Mono-glycerid wurde durch DC isoliert und zum Methylester umgewandelt. Die prozentualen Anteile der diesen Methylestern zugrundeliegenden Sauren betrugen: Palmitinsaure 11.8%, Stearinsaure 4.1%, Olsaure 12.8%, Linolsaure 3.7%, Arachidonsaure 8.3%, Eicosensaure 10.2%, Behensaure 6.5%, Docosensaure 4.2% und zwei unidentifizierte Sauren 22.2% und 16.2% Dieser nichtglyceridische Bestandteil von S. mukorossi Saatol ist ein Cyanolipid, 1-Cyano-2-hydroxymethylprop-1-en-3-ol. Die Struktur wurde durch IR, NMR und Massenspektroskopie bestatigt.

Study on Phytoconstituents and Antimicrobial Potential of Sapindus mukorossi Fruit Extract

Abstract: Sapindus mukorossiis well known for its folk medicinal values. The fruit is valued for the saponins (10.1%) present in the pericarp which constitutes up to 56.5% of the drupe known for inhibiting tumour cell growth. Recently many of the pharmacological actions of this plant have been explored which include the antimicrobial molluscicidal and insecticidal actions.Thefruitsareofconsiderableimportancefor their medicinal value for treatingseveraldiseaseslikeexcessivesalivation,pimples,epilepsy,chlorosis,migranes, eczemaand psoriasis etc.The powdered seeds are employed in the treatment ofdentalcaries, arthritis,commoncold, constipation and nausea. Keeping in view the medicinal importance of the fruit, it was thought worthwhile to carry out systematic study on phytochemical investigation and antimicrobial potential of fruit extract of Sapindus mukorossi.Fruit were dried, powdered and extracted with differentsolventsystemswithincreasingpolarity. The extracts were screened for the antibacterialandantifungalactivity.Predominance of antibacterial activity was observed at dose level of 50μg/ml of petroleum ether extract. Maximum zone of inhibition (19mm)&(18mm)wasobservedagainstSalmonellatyphiiand Shigelladysenteriarespectively as compared to standard drug Ampicillin20μg/ml.Sinceantibacterialactivity was observed in 50μg/ml concentration of petroleum ether extract, it was chosen forexploringitsantifungalpotentialalso.Maximum zone of inhibition was observed against Aspergillus sulfurous as compared to standard drug ketoconazole 20μg/ml. From the above study, it could be concluded that Sapindus mukorossipetroleum ether fruit extracts(50μg/ml) have good antimicrobial potential and can be explored further to isolate active principlesfrom the same.

Isolation and Characterization of Herbal Surfactant from Selected Medicinal Plant

Abstract: The shade dried fruits of Sapindus mukorossiwas extracted with pet ether, chloroform and ethyl acetatesuccessively by soxhlation method, water by maceration method at room temperature and Preliminary phytochemicals screening of extracts of fruits of Sapindusmukorossiwith various solvents.Then we isolate the compound with high purity by flash chromatography technique. Mobile phase Benzene: ethyl acetate: water (3:4:3) and we got 11 phytoconstituents separated in different test tube and characterized fraction with parameter UV, IR, and Surfactant properties. The above findings lead to the conclusion that the saponin was sussefully isolated in fraction as surfactant of S. mukorossi fruits.