S Ruggieri

Bio: S Ruggieri is an academic researcher from University of Florence. The author has contributed to research in topics: Gas chromatography & Saponification. The author has an hindex of 1, co-authored 1 publications receiving 24 citations.

Separation of the Methyl Esters of Fatty Acids by Thin Layer Chromatography

Abstract: IT is well known that the application of long-chain fatty acid samples to gas chromatography needs their previous esterification to methyl esters. Speaking generally, there are two ways to obtain such esters: (1) The esterification of the fatty acids with diazomethane, or methanol–hydrochloric acid, after saponification of the lipids and removal of the non-saponifiable compounds. (2) The direct transfer of the fatty acids in ester combination from glycerol to methanol1.

Recent advances of chromatography and mass spectrometry in lipidomics.

Abstract: Lipidomics, as a novel branch of metabolomics, which is aimed at comprehensive analysis of lipids and their biological roles with respect to health and diseases, has attracted increased attention from biological and analytical scientists. As a result of the complexity and diversity of lipids, accurate identification and efficient separation are required for lipidomics analysis. Mass spectrometry (MS) and chromatography have been extensively developed in the past few decades and hold a distinguished position in qualification and separation science. They are powerful and indispensable tools for lipidomics. Herein, we present the recent advancement of MS, chromatography, and their hyphenation technologies in lipidomics.

Use of Modified ‘Bentone-34’ for the Gas Chromatographic Separation of Aromatic Hydrocarbons

Abstract: The use of ‘Bentone-34’ (dimethyldioctadecylammonium bentonite) for the separation of aromatic hydrocarbons has been described previously1–3, but the chromatograms published have either exhibited considerable tailing2 or, where symmetrical peaks have been shown, these have been obtained using small sample volumes (about 0.02 µl.) and ionization detectors3,4.

Characterization of complex lipid mixtures in contaminant exposed JEG-3 cells using liquid chromatography and high-resolution mass spectrometry.

Abstract: The aim of this study was to develop a method based on ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) for lipid profiling in human placental choriocarcinoma (JEG-3) cells. Lipids were solid-liquid extracted from JEG-3 cells using a solution of chloroform/methanol (2:1, v/v) in a simple procedure requiring minimal sample alteration. Simultaneous separation of complex lipid mixtures in their major classes was achieved with a reversed-phase (C8) UHPLC column and a mobile phase containing methanol with 1 mM ammonium formate and 0.2 % formic acid (A)/water with 2 mM ammonium formate and 0.2 % formic acid (B). Lipids were characterized using time-of-flight (TOF) and Orbitrap under full scan and positive electrospray ionization mode with both analyzers. A total of 178 species of lipids, including 37 phosphatidylcholines (PC), 32 plasmalogen PC, 9 lyso PC, 4 lyso plasmalogen PC, 30 triacylglycerols, 22 diacylglycerols, 7 cholesterol esters, 25 phosphatidylethanolamines, and 12 sphingomyelins, were identified using TOF and Orbitrap. The identification of all lipid classes was based on exact mass characterization with an error < 5 ppm. The developed methodology was applied to study lipid alterations in human placental cells against the exposure to perfluorinated chemicals (PFCs) and tributyltin (TBT).